ID   DPOM_ASCIM              Reviewed;        1202 AA.
AC   P22374;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Probable DNA polymerase;
DE            EC=2.7.7.7;
OS   Ascobolus immersus.
OG   Mitochondrion.
OG   Plasmid pAI2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC   Pezizales; Ascobolaceae; Ascobolus.
OX   NCBI_TaxID=5191;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2/I;
RX   PubMed=2573821; DOI=10.1007/bf00332419;
RA   Kempken F., Meinhardt F., Esser K.;
RT   "In organello replication and viral affinity of linear, extrachromosomal
RT   DNA of the ascomycete Ascobolus immersus.";
RL   Mol. Gen. Genet. 218:523-530(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; X15982; CAA34106.1; -; Genomic_DNA.
DR   PIR; S05362; S05362.
DR   AlphaFoldDB; P22374; -.
DR   PRIDE; P22374; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR   InterPro; IPR015833; DNA-dir_DNA_pol_B_mt_lin_plsmd.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF03175; DNA_pol_B_2; 1.
DR   PIRSF; PIRSF006517; DPol_mt_plasmid; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Mitochondrion;
KW   Nucleotidyltransferase; Plasmid; Transferase.
FT   CHAIN           1..1202
FT                   /note="Probable DNA polymerase"
FT                   /id="PRO_0000046551"
SQ   SEQUENCE   1202 AA;  138280 MW;  51D41FCEBDBF2CDE CRC64;
     QFSIKVMQSS YVGLRSTVQF LQLSQIIWIK SRIAKSENDY INSMKKFLSH KKQNNRKFHT
     RSMSNGLGSL NLQKRPTPSP TVLNNKFLPT RNQYHSEDIN NNILFDIIIK ETDKGLKKCV
     LIGLNLTTNK IFNMEYIDLN TSNRKFKIFV ANNLINIKNN GINIDNLYIP KRINSKTLTD
     LITLSFPNIK VQWYLKSEIT NLFLKSDYTN FIKEINERKT LGKSILSKLI IYYNDSSPHC
     NIGKSSRKVT AISHTKRDIH TSTENNHKKD LFGWHRFNSS NYYDCIDSGA LTIEINKLFE
     YYKDNFTQPF FVVNAKIKFP TGNVRSIGFG NVTTLTDKET LIKTLAIFLE REDIHTVMSY
     DEGDIDESKF PKGSLSFDFK PLKTIEGTKY ANYTFPIKKD IVVKDINKKI NFNGLDLPKT
     MDLSKWPNLK LNKDKTSGEI RMTIKNKNNQ SYDIIGHMII NDGENVITFN RAVDNSIIKI
     FTVTDSMGNT NDPNLFKRIV EEKGNQTVYV YENNETVCVV QDKKFGFISK IAKNKTVNLK
     SISTLDLETR MDTNNRLIPI CMSYYNNKKL NTFLFKDDWQ EEMLAAFKTL LKSTNHGKKF
     YVHNLAHFDS VFILDTLSKL GKIDIIMRDD KIMKLKITFK IPGKNTEYSI SFLDSLLMLP
     NSLDNLSKAF NIENKKSVFP LKFTNGAVTP FNYIGAVPGY EYFYNTPNKK FTKDDYKKYC
     KDFNNNWDFN KELKNYCEID CLALHDILTL FAKMIHNEFS VDITRYVSLS SITFAIFRTN
     FLPENKIPNI TCTKLHYILK QAYTGGYCDV FKPEGKNIHS YDINSLYPSA MAKFDMPTGT
     PLHILGDPYK FTKDPFGFVF AEVTAPDLKV PIIQTRVQTS GGVRTVAPIG TFKGWYLLDE
     LINAKKYGYT FKISEAYLFE RLNIFKEYIH RLYTIKSSYT PDDPMYFIAK LLMNSLYGRF
     GMDPITIKYS VLTPEESEEK LKNSSCIEAT TLPSGNVLFK ENKPLGEFSN LNTSVPISAA
     IAAYSRMIMS EYLIKYADNL YAVDTDGIKV DTEIDKKYVS DKELGLMKHE YTFKEAVFVA
     PKVYGGLFDK PYKNKVEIVK VKGLKEPIQY SDLKDVYIKS LLKLFIILNF LRQLALSTIV
     IKEQPYSLRV SSFKRVLVFN ESGKIISTLP LKLENDKIVS NPHLIVNSEG NPSAFKLSLI
     KI