ID   DPOM_CLAPU              Reviewed;        1063 AA.
AC   P22373;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Probable DNA polymerase;
DE            EC=2.7.7.7;
OS   Claviceps purpurea (Ergot fungus) (Sphacelia segetum).
OG   Mitochondrion.
OG   Plasmid pClK1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Claviceps.
OX   NCBI_TaxID=5111;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K;
RX   PubMed=2770691; DOI=10.1007/bf00330952;
RA   Oeser B., Tudzynski P.;
RT   "The linear mitochondrial plasmid pClK1 of the phytopathogenic fungus
RT   Claviceps purpurea may code for a DNA polymerase and an RNA polymerase.";
RL   Mol. Gen. Genet. 217:132-140(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; X15648; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JQ0301; JQ0301.
DR   AlphaFoldDB; P22373; -.
DR   PRIDE; P22373; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR   InterPro; IPR015833; DNA-dir_DNA_pol_B_mt_lin_plsmd.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF03175; DNA_pol_B_2; 1.
DR   PIRSF; PIRSF006517; DPol_mt_plasmid; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Mitochondrion;
KW   Nucleotidyltransferase; Plasmid; Transferase.
FT   CHAIN           1..1063
FT                   /note="Probable DNA polymerase"
FT                   /id="PRO_0000046552"
SQ   SEQUENCE   1063 AA;  122541 MW;  3F5621BF3B6E147C CRC64;
     MLILLLQYNV SNPCLTNLLE NKTGTGVGLN FPLKPARLTL AQQRKRSELK KARLALRGMG
     IGDFNRELRF YSTSAYTKTH WNNKDRINKW MQYDIFVDRD FESFLSHLFS DNRILFEYTY
     ALLVKVRKGN KFYTIGDRQI GVLFVDIHDP FLLEVFDTII DRLAILFEEY PTELEYTCDA
     LLLEFHPVRP EKIKISNLKD AKEKLAPKTF ATGMSVFNLI GGFDLRKVST PINLNKKRYY
     STQVDRLNGF ITDNPRLLEF KQKFNEQLFM ASPKKKLKYE FCDTSVVFIL VKRGREYIVI
     EDVKDGEFHK RIFNKTASLL LHLIDKSNGD GTWTRINGNF NTLYNLENEI IMSTKAIGLR
     AIPKFLYNKK NEEEMSVLAN PNLGTIDLET TTGAQPKCYA IGFYTNLDSK PIKFYIDKNL
     DSCQLVHTCI QEMMRYKYRN VLFYAHNLGK FDAVFLLKEL LRFNLTTEGL NNPYEFPAPL
     TRNSDILKLT IKRTVDGKTR KVTIMDSFAL LPRSLRDLCK DYNIDNPKSY FPYLFSVDSH
     LFYTGATPNI NYYLDIPKAE YLKLVKDDWD FKDETLEYLN LDLISLHQVL VKVNKAINFL
     FDIEFTSCAT VSSLANKIFL SKFYDDKNKA IPLVKDIDLF NDIHEAYYGG RVEVFNPIIM
     ADSTKSYYYY DVNSLYPFAS INDIPGLKCT FYEVVKANVN IHELFGFFYC KIKSPDNLYL
     GLLPKRTETS LIFPGGAWEG WYFSEELKFA VAHGYEVEII KGYNFDRVSN VFNEFVQEVY
     KVKCNPNNVT EKNIAKLILN SLIGRFGMNI NKIKTSLVPS EKHNELLTTR VVKSTTDLGS
     GMFMDIYVPG IDKNICEDFN LDYIEVLNST NTDEKGTMPD NNVSITTAAA VLSYARIHMA
     QIMLFILENN GTLYYTDTDS IVTDLKLPEE MVHQTEIGKL KLEHTITQGY FIADKTYAIV
     NTEGEIIKRA KGVKSSKLTL EDYEKMYNKE VVEATKTSSK RNYKDGFVTI SDSTVKLNPT
     SYTKRSRVIS KDKWIGTEII RLDKLNSKLS THTPKSLSGD KTK