ID   DPOM_NEUIN              Reviewed;         969 AA.
AC   P33538;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Probable DNA polymerase;
DE            EC=2.7.7.7;
OS   Neurospora intermedia.
OG   Mitochondrion.
OG   Plasmid kalilo.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=5142;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1934129; DOI=10.1007/bf00326237;
RA   Chan B.S.S., Court D.A., Vierula P.J., Bertrand H.;
RT   "The kalilo linear senescence-inducing plasmid of Neurospora is an
RT   invertron and encodes DNA and RNA polymerases.";
RL   Curr. Genet. 20:225-237(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: The name 'Kalilo' originates from Hawai and means 'fatal
CC       disease' (because of the senescence induced by the plasmid).
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; X52106; CAA36327.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S17909; S17909.
DR   AlphaFoldDB; P33538; -.
DR   PRIDE; P33538; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR   InterPro; IPR015833; DNA-dir_DNA_pol_B_mt_lin_plsmd.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF03175; DNA_pol_B_2; 1.
DR   PIRSF; PIRSF006517; DPol_mt_plasmid; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Mitochondrion;
KW   Nucleotidyltransferase; Plasmid; Transferase.
FT   CHAIN           1..969
FT                   /note="Probable DNA polymerase"
FT                   /id="PRO_0000046555"
SQ   SEQUENCE   969 AA;  112830 MW;  6048B993814F668F CRC64;
     MSKFSFFNYR INMRNSHATL GSLPCFIKFN YLSSSSHSKF YSTSTDSPMG HIVSASKSSW
     YNYYSHKNYD PLTRESFHDE LRGFISLYKK QFKEEKFFFM AKIKFNNNDI RSISTVQIGS
     TDPLEVLRLL EAITSTYMHT HTGISEAVSE PFEYELFSLG DGLPKGNIIF TFKPTSNPSI
     KTKYEHKSNI KRNKNINLSK KNPLNKFKYN GYTIPNTMDL SQWPNIHFIN DGKNAVSLNN
     IIKSGVDNMT LSFFITINKK YNEITVLLNN TPIFKIKDEK IMSEDDLSSF KRTITENEQD
     KVYVFENGEM VFFSENVKTS FIKKITRQDL INFENPKIIT LDLETRSVPI HPIKEGKDGK
     EGKVDSIMFP ILMSVYNGKF VKSFLFSQSA WETEMMNAFK SIMLRKYDGY KVYTHNFSYF
     DGIFIIDILS RLGEVKPFMR NGKILKLTFN FTLPNSKRKY TLYFMDSLLI LPDSLDKLSN
     FFNNKVKKLF FPHSFLDDNT IPINYVGKCP DYKYFPKAYT EDFTIEQYQE YANKFKNNNW
     DLKKELIKYC EIDTIALYQV LVSFQRKIYE KFMIDCTKYP TIPSLAFAIF RKKYLVEDMI
     PNIKSKLHNI IKLSYFGGIC ELYKPFGVNI KSYDVNSLYP FAMKYFKMPS GIPKYVKGTL
     QNIVRFTDSI CEVPFGFYNV KVKTPLNLDK PFLPTRLNTP AGTRTAFPLG QWEGWYFSEE
     ILNAMKHGYE FEFIEGYLFE ESSMFDEYID LLYNIKKNSP KESPWYYISK LLMNSLYGRF
     GLNPEGEEIF ITSEEEGDAI IATKEYVTIT PLSSGNVLIS AKLPEEAFGD MNISVPISSA
     IAAYSRIHMS HFLTKYSNNI YYIDTDGIKV DIDLDKDEVD SKELGKMKYE YVFEEYTSLG
     PKVYGGLLYD KKGKLIELVK LRGYSSKLPY NKLKEGLVKD HTLELTQKKW KRKLSESTVY
     LKNTHLLFL