ID   DPOM_PODAS              Reviewed;        1197 AA.
AC   Q01529;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Probable DNA polymerase;
DE            EC=2.7.7.7;
OS   Podospora anserina (Pleurage anserina).
OG   Mitochondrion.
OG   Plasmid pAL2-1.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Podosporaceae; Podospora.
OX   NCBI_TaxID=2587412;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AL2;
RX   PubMed=1473181; DOI=10.1007/bf00326415;
RA   Hermanns J., Osiewacz H.D.;
RT   "The linear mitochondrial plasmid pAL2-1 of a long-lived Podospora anserina
RT   mutant is an invertron encoding a DNA and RNA polymerase.";
RL   Curr. Genet. 22:491-500(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- MISCELLANEOUS: This DNA polymerase requires a protein as a primer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; X60707; CAA43117.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q01529; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR004868; DNA-dir_DNA_pol_B_mt/vir.
DR   InterPro; IPR015833; DNA-dir_DNA_pol_B_mt_lin_plsmd.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF03175; DNA_pol_B_2; 1.
DR   PIRSF; PIRSF006517; DPol_mt_plasmid; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Mitochondrion;
KW   Nucleotidyltransferase; Plasmid; Transferase.
FT   CHAIN           1..1197
FT                   /note="Probable DNA polymerase"
FT                   /id="PRO_0000046556"
SQ   SEQUENCE   1197 AA;  139335 MW;  5EA8C9D9719BB431 CRC64;
     MKKNNTILYY GCAAQARYAS SNHGLLYSPS QAHLFNFSLA RTYLNFSNGF RAFSSERKRQ
     NSWKLSVRSI YMDSVNFTKH DFINALLKNF KQGNVYTVLV KVEYLDSSGN RAWCMLNVQK
     GIIYRNKSDF IELVKEFFDE VYIGYIEYMS RYHAQEVLSI QIMYILNNDS YRQYSLKNIN
     KEKLDKNLVN VKQTKLDFSE SLLPLTTNEK FYGKRLTPIL DYKKQFVTSL YINGINFIKL
     VNKKSKDLNQ EFINFDSKTR FYRYKLNEIE YIITVSNISK TETVKTIYLM TGFKFKDNIL
     DKELTTKIFS RQIGNTTIEF DGADIINKEI KLKLPIIRLN YKPFTDLNSR IGTFDLETFR
     DYNSNSAVYA LGFSTLSMSK TDKKTSMYYL TKDGNTSHEI IIKCINEMLS SDYRDHIYFT
     HNLGGYDIIF ILHALKLENK IILENKLKGI NTIVEDDKKI KVKKKKPISD VNKKSQNKDH
     YEISTILRDD RILKCVIKVK TPSGYNKITF IDSYNILPDK LDNLAKSFGT EIQKGLFPYE
     FVKSNTLNYV GITPSIEYYK INNEVISQEL YNELIVPQWD LRKQTLHYLE RDLLSLLEII
     NTYNHYVYKR YNVQLTESLT IARLALNIYL KRYLGDNLIP VVLNNSLFTS IKAAYYGGVA
     EVYRPYGKNL RYYDVNSLYP FVAKNTMPGH ECKYIESKKG LKLSELFGFF YCKVTTNNQY
     LGLLPVHNQG LIMPNGQWYG WYFSEELKFA EVNGYNIEVI KGYQFNKIDN LFSSYVDDLY
     KIKANSEGSE KLITKFLLNS LLGRFGMSIF KLKTDIVSVE KAKKLAVTNY INSVKAISDT
     DVLISYNKEI SRKLVEEHGL NYIEILNSNS KLDLEKNNSF KDVAVSISAA VTAYARIFMA
     QTKLDILKNG GNLYYTDTDS IVTDIDLPDN LVGSELGQFK LEFKLKEGFF ISAKTYCLIL
     EKEYIKKNKN KDTVIKAKGV FKTSLDVEKF KSLYFNKQDV EAIKSNNKTN YLEGYVNIES
     GIVKLKHDAY TKRSKIYDNN GLWIDTKPLN YQVSNMLEDT NSKPLNSPNN NDLCALIKYN
     KPCFDLIKYN KSITDLILYL PTNKAKFINY AQLESMINEA IHESDMRVAN HIKMLIYSLT
     GILYHGKDGT MYIYSPNSKK IYIKYKKNNK WVTFWNIKYH NGYVYIKNKN KFYLYKG