DPP3_DICDI
ID DPP3_DICDI Reviewed; 691 AA.
AC Q557H1; Q8T1P0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Dipeptidyl peptidase 3;
DE EC=3.4.14.4;
DE AltName: Full=Dipeptidyl aminopeptidase III;
DE AltName: Full=Dipeptidyl arylamidase III;
DE AltName: Full=Dipeptidyl peptidase III;
DE Short=DPP III;
GN Name=dpp3-1; ORFNames=DDB_G0273471;
GN and
GN Name=dpp3-2; ORFNames=DDB_G0273563;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal dipeptide from a peptide comprising
CC four or more residues, with broad specificity. Also acts on
CC dipeptidyl 2-naphthylamides.; EC=3.4.14.4;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:Q9NY33};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:Q9NY33};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase M49 family. {ECO:0000305}.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC AX4. These strains contain a duplication of a segment of 750 kb of
CC chromosome 2 compared to the corresponding sequence in strain AX2.
CC {ECO:0000305}.
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DR EMBL; AAFI02000010; EAL70690.1; -; Genomic_DNA.
DR EMBL; AAFI02000010; EAL70736.1; -; Genomic_DNA.
DR RefSeq; XP_644587.1; XM_639495.1.
DR RefSeq; XP_644664.1; XM_639572.1.
DR AlphaFoldDB; Q557H1; -.
DR SMR; Q557H1; -.
DR STRING; 44689.DDB0266801; -.
DR MEROPS; M49.A02; -.
DR PaxDb; Q557H1; -.
DR PRIDE; Q557H1; -.
DR EnsemblProtists; EAL70690; EAL70690; DDB_G0273563.
DR EnsemblProtists; EAL70736; EAL70736; DDB_G0273471.
DR GeneID; 8618951; -.
DR GeneID; 8619026; -.
DR KEGG; ddi:DDB_G0273471; -.
DR KEGG; ddi:DDB_G0273563; -.
DR dictyBase; DDB_G0273471; dpp3-1.
DR dictyBase; DDB_G0273563; dpp3-2.
DR eggNOG; KOG3675; Eukaryota.
DR HOGENOM; CLU_011977_1_0_1; -.
DR InParanoid; Q557H1; -.
DR OMA; HCQARFA; -.
DR PhylomeDB; Q557H1; -.
DR PRO; PR:Q557H1; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; ISS:dictyBase.
DR GO; GO:0008239; F:dipeptidyl-peptidase activity; ISS:dictyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; ISS:dictyBase.
DR InterPro; IPR005317; Dipeptidyl-peptase3.
DR InterPro; IPR039461; Peptidase_M49.
DR PANTHER; PTHR23422; PTHR23422; 1.
DR Pfam; PF03571; Peptidase_M49; 1.
DR PIRSF; PIRSF007828; Dipeptidyl-peptidase_III; 1.
PE 3: Inferred from homology;
KW Aminopeptidase; Cytoplasm; Hydrolase; Metal-binding; Metalloprotease;
KW Protease; Reference proteome; Zinc.
FT CHAIN 1..691
FT /note="Dipeptidyl peptidase 3"
FT /id="PRO_0000332967"
FT ACT_SITE 432
FT /evidence="ECO:0000250|UniProtKB:O55096"
FT BINDING 431
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9NY33"
FT BINDING 436
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9NY33"
FT BINDING 492
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250|UniProtKB:Q9NY33"
SQ SEQUENCE 691 AA; 78274 MW; 94C2097F73312E82 CRC64;
MSVPSSVIEN HLVPKEIPVY RLNARESFNL LTEKEQLYAH HISVACWWGS KICLGQTSIE
SGPIFNLFQN LFSIQNLKST VVPNIVSEEE YSDLLSYAAT FYGNMGNYLS FGDSKFIPRI
SKEKLQLIIN KVNDNKVNEY WGKCSELMYS LDKQVRELGI DGNGISTYYS PNITKVEIEK
VQKFMDSKSI SPYNTRLFKV SENNYNLLIA SASTSTPTVS HQFDGYTINI VYGDWNKNLT
KVVDNLKLAL PYAANENQTN MLKKYIDSFY SGSIDDHKDS QRWWIKDISP AVETNIGFIE
SYRDPYGVRG EWEGFVSMVN KEMSLKFGKL TDNATTFLSK LPWDKSFEKE KFNKPDFTSL
EVLTFATTGI PAGINLSNYD DIRQTEGFKN VSLGNVIAAR KDEYVTFIQE SDQKLFNELS
TEAFELQVGI HELYGHGSGK LFTTDANGNV NFKVGEVINP LTNKPIDPKT EVYKFGETYD
SVFKSLGSPM EECRAECCGI YLSPDEKILE LFGFTDPKKA EDVYYVNWLI MARAGVCALE
FYSPPSEGAP GKWRQAHMQA RYCILTTFLR SGIVTLDKTA DDVIVKLDKS KIRGIGVKAV
GDFLNRLMVY KATANIDASI KLFDEYTHVN EEFLAIRDIV LAKKKPRKVF VQAHTYLNSN
GKVCLQDFDD STQGMIDSMI TRFGKDDSDM L
