ID   DPPA1_PSEAB             Reviewed;         537 AA.
AC   A0A0H2ZGV2;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 24.
DE   RecName: Full=Di/tripeptide-binding protein 1 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=dppA1 {ECO:0000303|PubMed:25338022};
GN   OrderedLocusNames=PA14_58350 {ECO:0000312|EMBL:ABJ13764.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=25338022; DOI=10.1371/journal.pone.0111311;
RA   Pletzer D., Lafon C., Braun Y., Koehler T., Page M.G., Mourez M.,
RA   Weingart H.;
RT   "High-throughput screening of dipeptide utilization mediated by the ABC
RT   transporter DppBCDF and its substrate-binding proteins DppA1-A5 in
RT   Pseudomonas aeruginosa.";
RL   PLoS ONE 9:e111311-e111311(2014).
CC   -!- FUNCTION: Part of the ABC transporter DppABCDF involved in the uptake
CC       of various di/tripeptides (PubMed:25338022). Prefers dipeptides with
CC       acidic residues at the C-terminal end. Involved in the uptake of
CC       phaseolotoxin, a toxic tripeptide inhibiting the enzyme ornithine
CC       carbamoyltransferase (PubMed:25338022). {ECO:0000269|PubMed:25338022}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (DppD and
CC       DppF), two transmembrane proteins (DppB and DppC) and a solute-binding
CC       protein (DppA1) (PubMed:25338022). Five orthologous SBPs (DppA1-A5) are
CC       present in P.aeruginosa, which increases the substrate specificity of
CC       the DppBCDF transporter (PubMed:25338022).
CC       {ECO:0000269|PubMed:25338022}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CP000438; ABJ13764.1; -; Genomic_DNA.
DR   RefSeq; WP_003116231.1; NZ_CP034244.1.
DR   AlphaFoldDB; A0A0H2ZGV2; -.
DR   SMR; A0A0H2ZGV2; -.
DR   EnsemblBacteria; ABJ13764; ABJ13764; PA14_58350.
DR   KEGG; pau:PA14_58350; -.
DR   HOGENOM; CLU_017028_7_0_6; -.
DR   OMA; YIAYNVM; -.
DR   BioCyc; PAER208963:G1G74-4914-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR   GO; GO:0015833; P:peptide transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR030678; Peptide/Ni-bd.
DR   InterPro; IPR039424; SBP_5.
DR   InterPro; IPR023765; SBP_5_CS.
DR   InterPro; IPR000914; SBP_5_dom.
DR   PANTHER; PTHR30290; PTHR30290; 1.
DR   Pfam; PF00496; SBP_bac_5; 1.
DR   PIRSF; PIRSF002741; MppA; 1.
DR   PROSITE; PS01040; SBP_BACTERIAL_5; 1.
PE   1: Evidence at protein level;
KW   Peptide transport; Protein transport; Signal; Transport.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..537
FT                   /note="Di/tripeptide-binding protein 1"
FT                   /id="PRO_0000452188"
SQ   SEQUENCE   537 AA;  60109 MW;  9EDDF6944A57730D CRC64;
     MRRNAVIRSA IMPSLLGAAL VAAVPQAFAS NLIFCSEGSP AGFDPAQYTT GTDFDAAAET
     VFNRLTQFER GGTKVLPGLA ESWDVSDDGK TYTFHLRKGV KFHSTDYFKP TREFNADDVL
     FTFERMLDKD HPFRKAYPTE FPYFTDMGLD KNIARIEKLD EHTVKFTLNE VDAAFIQNLA
     MPFPSIQSAE YAAQLLKQGK ASDINQKPIG TGPFVFSRYQ KDAQIRFKGN KDYWKPDEVK
     VDNLIFAINT DASVRAQKLK AGECQITLNP RPADLDALKK DPNLNLPSQA GFNLGYIAYN
     VTHKPFDKLE VRQALDMAVN KQAIIDAVYQ GAGQLASNGM PPTQWSYDET IKDAPYDPAK
     ARELLKKAGV AEGTEITLWA MPVQRPYNPN AKLMAEMLQN DWAKIGIKAK IVTYEWGEYI
     KRAKGGEHDA MLIGWSGDNG DPDNWLGTLY GCDAVDGNNF SKWCDAGYDK LVKDAKRTTD
     QGKRTELYKQ AQHILKEQVP ITPIAHSTVY QPMRKTVHDF RISPFGLNSF YEVSVGK