DPPA2_MOUSE
ID DPPA2_MOUSE Reviewed; 301 AA.
AC Q9CWH0;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Developmental pluripotency-associated protein 2;
DE AltName: Full=Embryonic stem cell-associated transcript 15-2 protein;
DE Short=ECAT15-2;
DE AltName: Full=Pluripotent embryonic stem cell-related gene 1 protein;
GN Name=Dppa2; Synonyms=Phsecrg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12620990; DOI=10.1242/dev.00366;
RA Bortvin A., Eggan K., Skaletsky H., Akutsu H., Berry D.L., Yanagimachi R.,
RA Page D.C., Jaenisch R.;
RT "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from
RT somatic nuclei.";
RL Development 130:1673-1680(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15583978;
RA Du J., Lin G., Nie Z.Y., Lu G.X.;
RT "Molecular cloning and characterization analysis of HPESCRG1, a novel gene
RT expressed specifically in human embryonic stem cell.";
RL Zhonghua Yi Xue Yi Chuan Xue Za Zhi 21:542-547(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Blastocyst, Egg, and Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP FUNCTION, INTERACTION WITH DPPA4, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21896782; DOI=10.1128/mcb.05701-11;
RA Nakamura T., Nakagawa M., Ichisaka T., Shiota A., Yamanaka S.;
RT "Essential roles of ECAT15-2/Dppa2 in functional lung development.";
RL Mol. Cell. Biol. 31:4366-4378(2011).
CC -!- FUNCTION: Binds to target gene promoters, including NKX2-5 and SYCE1,
CC but not GATA4, and may be involved in the maintenance of the active
CC epigenetic status of these genes. {ECO:0000269|PubMed:21896782}.
CC -!- SUBUNIT: Interacts with DPPA4. {ECO:0000269|PubMed:21896782}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21896782}.
CC -!- TISSUE SPECIFICITY: Not detected in adult tissues.
CC {ECO:0000269|PubMed:12620990}.
CC -!- DEVELOPMENTAL STAGE: Expressed in 4-cell embryo, morula and blastocyst.
CC At 7.5 dpc, detected in the epiblast. At 10.0 dpc, expressed in the
CC primordial germ cells. Expression decreases at 12.0 dpc in the
CC embryonic gonads. At 14.5 dpc, expression is restricted to testis. 6
CC days after birth, still detected in primitive type A spermatogonia.
CC Expressed in undifferentiated embryonic stem cells, but expression
CC rapidly decreases upon differentiation. {ECO:0000269|PubMed:12620990,
CC ECO:0000269|PubMed:21896782}.
CC -!- DISRUPTION PHENOTYPE: Mutant animals show survival rates similar to
CC wild-type until 16.5 dpc. Then mortality increases and no animals
CC survive beyond weaning age. At 18.5 dpc, the lungs of mutants mice show
CC a thicker mesenchyme and smaller alveolar space than those of wild-type
CC animals. {ECO:0000269|PubMed:21896782}.
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DR EMBL; AF490346; AAO84504.1; -; mRNA.
DR EMBL; AY561246; AAS68013.1; -; mRNA.
DR EMBL; AK010743; BAB27153.1; -; mRNA.
DR EMBL; AK145760; BAE26632.1; -; mRNA.
DR EMBL; AK145645; BAE26562.1; -; mRNA.
DR EMBL; AK163375; BAE37324.1; -; mRNA.
DR EMBL; AK167211; BAE39338.1; -; mRNA.
DR CCDS; CCDS37350.1; -.
DR RefSeq; NP_082891.1; NM_028615.1.
DR AlphaFoldDB; Q9CWH0; -.
DR SMR; Q9CWH0; -.
DR STRING; 10090.ENSMUSP00000110183; -.
DR iPTMnet; Q9CWH0; -.
DR PhosphoSitePlus; Q9CWH0; -.
DR MaxQB; Q9CWH0; -.
DR PaxDb; Q9CWH0; -.
DR PRIDE; Q9CWH0; -.
DR ProteomicsDB; 277492; -.
DR Antibodypedia; 32413; 309 antibodies from 31 providers.
DR DNASU; 73703; -.
DR Ensembl; ENSMUST00000097175; ENSMUSP00000110183; ENSMUSG00000072419.
DR Ensembl; ENSMUST00000232448; ENSMUSP00000156143; ENSMUSG00000072419.
DR GeneID; 73703; -.
DR KEGG; mmu:73703; -.
DR UCSC; uc007zjn.1; mouse.
DR CTD; 151871; -.
DR MGI; MGI:2157523; Dppa2.
DR VEuPathDB; HostDB:ENSMUSG00000072419; -.
DR eggNOG; ENOG502RVK6; Eukaryota.
DR GeneTree; ENSGT00390000004871; -.
DR HOGENOM; CLU_080062_1_0_1; -.
DR InParanoid; Q9CWH0; -.
DR OMA; FCPKHSC; -.
DR OrthoDB; 1295613at2759; -.
DR PhylomeDB; Q9CWH0; -.
DR TreeFam; TF338129; -.
DR BioGRID-ORCS; 73703; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Dppa2; mouse.
DR PRO; PR:Q9CWH0; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CWH0; protein.
DR Bgee; ENSMUSG00000072419; Expressed in cleaving embryo and 20 other tissues.
DR ExpressionAtlas; Q9CWH0; baseline and differential.
DR Genevisible; Q9CWH0; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0060484; P:lung-associated mesenchyme development; IMP:MGI.
DR GO; GO:2000648; P:positive regulation of stem cell proliferation; IMP:MGI.
DR GO; GO:0031060; P:regulation of histone methylation; IMP:MGI.
DR GO; GO:0019827; P:stem cell population maintenance; IMP:MGI.
DR GO; GO:0048731; P:system development; IBA:GO_Central.
DR InterPro; IPR039835; Dppa2.
DR InterPro; IPR039590; Dppa2/4.
DR InterPro; IPR025891; Dppa2/4_C_dom.
DR InterPro; IPR025892; Dppa2/4_central_dom.
DR PANTHER; PTHR16073; PTHR16073; 1.
DR PANTHER; PTHR16073:SF10; PTHR16073:SF10; 1.
DR Pfam; PF14047; DCR; 1.
DR Pfam; PF14049; Dppa2_A; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..301
FT /note="Developmental pluripotency-associated protein 2"
FT /id="PRO_0000239264"
FT DOMAIN 85..119
FT /note="SAP"
FT REGION 31..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..55
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 301 AA; 34116 MW; 3DAC9C0EE94984E8 CRC64;
MSYFGLETFN ENQSEENLDE ESVILTLVPF KEEEEPNTDY ATQSNVSSST LDHTPPARSL
VRHAGIKHPT RTIPSTCPPP SLPPIRDVSR NTLREWCRYH NLSTDGKKVE VYLRLRRHSY
SKQECYIPNT SREARMKQGP KKSKIVFRGI GPPSGCQRKK EESGVLEILT SPKESTFAAW
ARIAMRAAQS MSKNRCPLPS NVEAFLPQAT GSRWCVVHGR QLPADKKGWV RLQFLAGQTW
VPDTPQRMNF LFLLPACIIP EPGVEDNLLC PECVHSNKKI LRNFKIRSRA KKNALPPNMP
P
