ID   DPPA2_PSEAB             Reviewed;         532 AA.
AC   A0A0H2ZGW2;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 23.
DE   RecName: Full=Di/tripeptide-binding protein 2 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=dppA2 {ECO:0000303|PubMed:25338022};
GN   OrderedLocusNames=PA14_58360 {ECO:0000312|EMBL:ABJ13765.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=25338022; DOI=10.1371/journal.pone.0111311;
RA   Pletzer D., Lafon C., Braun Y., Koehler T., Page M.G., Mourez M.,
RA   Weingart H.;
RT   "High-throughput screening of dipeptide utilization mediated by the ABC
RT   transporter DppBCDF and its substrate-binding proteins DppA1-A5 in
RT   Pseudomonas aeruginosa.";
RL   PLoS ONE 9:e111311-e111311(2014).
CC   -!- FUNCTION: Part of the ABC transporter DppABCDF involved in the uptake
CC       of various di/tripeptides (PubMed:25338022). Shows high flexibility on
CC       substrate recognition. Efficiently uses tripeptides (PubMed:25338022).
CC       {ECO:0000269|PubMed:25338022}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (DppD and
CC       DppF), two transmembrane proteins (DppB and DppC) and a solute-binding
CC       protein (DppA2) (PubMed:25338022). Five orthologous SBPs (DppA1-A5) are
CC       present in P.aeruginosa, which increases the substrate specificity of
CC       the DppBCDF transporter (PubMed:25338022).
CC       {ECO:0000269|PubMed:25338022}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CP000438; ABJ13765.1; -; Genomic_DNA.
DR   RefSeq; WP_003141332.1; NZ_CP034244.1.
DR   AlphaFoldDB; A0A0H2ZGW2; -.
DR   SMR; A0A0H2ZGW2; -.
DR   EnsemblBacteria; ABJ13765; ABJ13765; PA14_58360.
DR   KEGG; pau:PA14_58360; -.
DR   HOGENOM; CLU_017028_7_0_6; -.
DR   OMA; YIPLTYQ; -.
DR   BioCyc; PAER208963:G1G74-4915-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:UniProt.
DR   GO; GO:0015833; P:peptide transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR030678; Peptide/Ni-bd.
DR   InterPro; IPR039424; SBP_5.
DR   InterPro; IPR000914; SBP_5_dom.
DR   PANTHER; PTHR30290; PTHR30290; 1.
DR   Pfam; PF00496; SBP_bac_5; 1.
DR   PIRSF; PIRSF002741; MppA; 1.
PE   1: Evidence at protein level;
KW   Peptide transport; Protein transport; Signal; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..532
FT                   /note="Di/tripeptide-binding protein 2"
FT                   /id="PRO_0000452189"
SQ   SEQUENCE   532 AA;  59467 MW;  FABCCAAD682331FB CRC64;
     MRPRSALRYS LLLLAFAASA AIQAQPKTLA VCTEAAPEGF DPARYTSGYT FDASAHPLYN
     ALAAFAPGSA TVIPALAESW DVSADGLVYT FRLRQGVKFH STDYFKPSRE FNADDVLFSF
     QRMLDPQHPA HDLSPSGYPY ADAMQLRDII ERIEKIDEHQ VRFVLKHPEA PFLADLAMPF
     GSILSAEYAG QLIARGKGDE LNSKPIGTGP FVFTRYRKDA QVRYAANPDY WKGKPAIDHL
     VLAITLDPNV RVQRLRRNEC QIALTPKPED VAALRQDPQL TVLEEAAMIT SHAAINTRHE
     PFDDPRVRRA IAMGFNKSSY LKIVFGDQAR PAIGPYPPML LGYDDSIRDW PYDPERAKAL
     LKEAGVAPDT PLNLYISTGS GPGGNPARVA QLIQSDLAAI GIRVNIHQFE WGEMVKRTKA
     GEHDMMLYSW IGDNGDPDNF LTHNLGCASV ESGENRARWC DKGFDEAIRK ARMSNDESQR
     VALYKEAQRI FHEQMPWLPL AHPLMFDAQR KNVSGYRMSP MSARDFSRVK LD