DPPA3_MOUSE
ID DPPA3_MOUSE Reviewed; 150 AA.
AC Q8QZY3; F6S6F5;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Developmental pluripotency-associated protein 3;
DE AltName: Full=Compaction-associated protein 1;
DE AltName: Full=Primordial germ cell protein 7;
GN Name=Dppa3; Synonyms=Cap1p, Crg1, Pgc7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=129/SvEv;
RX PubMed=12124616; DOI=10.1038/nature00927;
RA Saitou M., Barton S.C., Surani M.A.;
RT "A molecular programme for the specification of germ cell fate in mice.";
RL Nature 418:293-300(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=11900980; DOI=10.1016/s0925-4773(02)00002-3;
RA Sato M., Kimura T., Kurokawa K., Fujita Y., Abe K., Masuhara M.,
RA Yasunaga T., Ryo A., Yamamoto M., Nakano T.;
RT "Identification of PGC7, a new gene expressed specifically in
RT preimplantation embryos and germ cells.";
RL Mech. Dev. 113:91-94(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=12620990; DOI=10.1242/dev.00366;
RA Bortvin A., Eggan K., Skaletsky H., Akutsu H., Berry D.L., Yanagimachi R.,
RA Page D.C., Jaenisch R.;
RT "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from
RT somatic nuclei.";
RL Development 130:1673-1680(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=KM;
RA Li W., Lu G.;
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Oocyte;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=14654002; DOI=10.1016/j.cub.2003.11.026;
RA Payer B., Saitou M., Barton S.C., Thresher R., Dixon J.P., Zahn D.,
RA Colledge W.H., Carlton M.B., Nakano T., Surani M.A.;
RT "Stella is a maternal effect gene required for normal early development in
RT mice.";
RL Curr. Biol. 13:2110-2117(2003).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=14684992; DOI=10.1159/000074346;
RA Bowles J., Teasdale R.P., James K., Koopman P.;
RT "Dppa3 is a marker of pluripotency and has a human homologue that is
RT expressed in germ cell tumours.";
RL Cytogenet. Genome Res. 101:261-265(2003).
RN [10]
RP DISRUPTION PHENOTYPE.
RX PubMed=15018652; DOI=10.1186/1471-213x-4-2;
RA Bortvin A., Goodheart M., Liao M., Page D.C.;
RT "Dppa3 / Pgc7 / stella is a maternal factor and is not required for germ
RT cell specification in mice.";
RL BMC Dev. Biol. 4:2-2(2004).
RN [11]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP LEU-44 AND LEU-46.
RX PubMed=17143267; DOI=10.1038/ncb1519;
RA Nakamura T., Arai Y., Umehara H., Masuhara M., Kimura T., Taniguchi H.,
RA Sekimoto T., Ikawa M., Yoneda Y., Okabe M., Tanaka S., Shiota K.,
RA Nakano T.;
RT "PGC7/Stella protects against DNA demethylation in early embryogenesis.";
RL Nat. Cell Biol. 9:64-71(2007).
RN [12]
RP FUNCTION.
RX PubMed=21407207; DOI=10.1038/ncomms1240;
RA Wossidlo M., Nakamura T., Lepikhov K., Marques C.J., Zakhartchenko V.,
RA Boiani M., Arand J., Nakano T., Reik W., Walter J.;
RT "5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic
RT reprogramming.";
RL Nat. Commun. 2:241-241(2011).
RN [13]
RP FUNCTION.
RX PubMed=22034526; DOI=10.1095/biolreprod.111.095018;
RA Liu Y.J., Nakamura T., Nakano T.;
RT "Essential role of DPPA3 for chromatin condensation in mouse
RT oocytogenesis.";
RL Biol. Reprod. 86:40-40(2012).
RN [14]
RP FUNCTION, H3K9ME2 METHYLATED HISTONE-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=22722204; DOI=10.1038/nature11093;
RA Nakamura T., Liu Y.J., Nakashima H., Umehara H., Inoue K., Matoba S.,
RA Tachibana M., Ogura A., Shinkai Y., Nakano T.;
RT "PGC7 binds histone H3K9me2 to protect against conversion of 5mC to 5hmC in
RT early embryos.";
RL Nature 486:415-419(2012).
CC -!- FUNCTION: Primordial germ cell (PGCs)-specific protein involved in
CC epigenetic chromatin reprogramming in the zygote following
CC fertilization. In zygotes, DNA demethylation occurs selectively in the
CC paternal pronucleus before the first cell division, while the adjacent
CC maternal pronucleus and certain paternally-imprinted loci are protected
CC from this process. Participates in protection of DNA methylation in the
CC maternal pronucleus by preventing conversion of 5mC to 5hmC:
CC specifically recognizes and binds histone H3 dimethylated at 'Lys-9'
CC (H3K9me2) on maternal genome, and protects maternal genome from TET3-
CC mediated conversion to 5hmC and subsequent DNA demethylation. Does not
CC bind paternal chromatin, which is mainly packed into protamine and does
CC not contain much H3K9me2 mark. Also protects imprinted loci that are
CC marked with H3K9me2 in mature sperm from DNA demethylation in early
CC embryogenesis. May be important for the totipotent/pluripotent states
CC continuing through preimplantation development. Also involved in
CC chromatin condensation in oocytogenesis. {ECO:0000269|PubMed:12124616,
CC ECO:0000269|PubMed:12620990, ECO:0000269|PubMed:14654002,
CC ECO:0000269|PubMed:17143267, ECO:0000269|PubMed:21407207,
CC ECO:0000269|PubMed:22034526, ECO:0000269|PubMed:22722204}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localized in the
CC cytoplasm at the primary oocyte stage and in oocytes within mono-
CC laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in
CC bi-laminar and Graafian follicles and during the 2-cell and morula
CC stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly
CC localizes in the female pronucleus, localization to the male pronucleus
CC in much weaker.
CC -!- TISSUE SPECIFICITY: Expressed in the immature oocytes and in newborn
CC ovaries. Subsequently detected in maturing oocytes and in
CC preimplantation embryos. Expressed in pluripotent embryonic but not in
CC differentiated somatic cells. Expressed in blastocysts, epiblasts,
CC primordial germ cells, embryonic gonads and primitive spermatogonia. No
CC expression is detected in adult testes. {ECO:0000269|PubMed:11900980,
CC ECO:0000269|PubMed:12124616, ECO:0000269|PubMed:12620990}.
CC -!- DEVELOPMENTAL STAGE: Detected at 3.5 dpc (at protein level). Activated
CC during the process of germ cell specification at 7 dpc.25, specifically
CC in the founder population of lineage-restricted primordial germ cells
CC (PGCs). Thereafter, expressed in the germ line until about 15.5 dpc in
CC male and 13.5 dpc in female gonads. Expressed during blastocyst, morula
CC and 4-cell embryo stages. {ECO:0000269|PubMed:11900980,
CC ECO:0000269|PubMed:12620990, ECO:0000269|PubMed:14654002,
CC ECO:0000269|PubMed:14684992}.
CC -!- DOMAIN: Mediates binding to H3K9me2 via N-terminal region, while
CC ability to exclude TET3 from the maternal pronucleus requires the C-
CC terminal part. {ECO:0000269|PubMed:22722204}.
CC -!- DISRUPTION PHENOTYPE: Null mutation result in apparently normal
CC offspring. No effect on early gonadal PGCs or gross abnormalities in
CC the development of gametes. However, females display severely reduced
CC fertility despite ovulation of normal numbers of oocytes. Null mutation
CC resulted in preimplantation development failure. Embryos rarely reached
CC the blastocyst stage. {ECO:0000269|PubMed:14654002,
CC ECO:0000269|PubMed:15018652, ECO:0000269|PubMed:17143267}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY082485; AAM03317.1; -; mRNA.
DR EMBL; AB072734; BAB86304.1; -; mRNA.
DR EMBL; AF490347; AAO84505.1; -; mRNA.
DR EMBL; AY134859; AAN12283.1; -; mRNA.
DR EMBL; AK135769; BAE22650.1; -; mRNA.
DR EMBL; AK136263; BAE22904.1; -; mRNA.
DR EMBL; AC158651; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC099433; AAH99433.1; -; mRNA.
DR EMBL; BC100331; AAI00332.1; -; mRNA.
DR EMBL; BC107340; AAI07341.1; -; mRNA.
DR EMBL; BC107341; AAI07342.1; -; mRNA.
DR CCDS; CCDS20500.1; -.
DR RefSeq; NP_631964.1; NM_139218.1.
DR AlphaFoldDB; Q8QZY3; -.
DR BioGRID; 216201; 308.
DR DIP; DIP-59897N; -.
DR STRING; 10090.ENSMUSP00000062832; -.
DR iPTMnet; Q8QZY3; -.
DR PhosphoSitePlus; Q8QZY3; -.
DR PaxDb; Q8QZY3; -.
DR PRIDE; Q8QZY3; -.
DR ProteomicsDB; 277399; -.
DR Antibodypedia; 22960; 172 antibodies from 31 providers.
DR DNASU; 73708; -.
DR Ensembl; ENSMUST00000049644; ENSMUSP00000062832; ENSMUSG00000046323.
DR GeneID; 73708; -.
DR KEGG; mmu:73708; -.
DR UCSC; uc009dpn.1; mouse.
DR CTD; 359787; -.
DR MGI; MGI:1920958; Dppa3.
DR VEuPathDB; HostDB:ENSMUSG00000046323; -.
DR GeneTree; ENSGT00800000124303; -.
DR HOGENOM; CLU_107188_1_0_1; -.
DR InParanoid; Q8QZY3; -.
DR OMA; GICCKAT; -.
DR OrthoDB; 1539071at2759; -.
DR PhylomeDB; Q8QZY3; -.
DR TreeFam; TF338511; -.
DR BioGRID-ORCS; 73708; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Dppa3; mouse.
DR PRO; PR:Q8QZY3; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8QZY3; protein.
DR Bgee; ENSMUSG00000046323; Expressed in morula and 60 other tissues.
DR ExpressionAtlas; Q8QZY3; baseline and differential.
DR Genevisible; Q8QZY3; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0001939; C:female pronucleus; IDA:UniProtKB.
DR GO; GO:0001940; C:male pronucleus; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0035064; F:methylated histone binding; IMP:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0040016; P:embryonic cleavage; IMP:MGI.
DR GO; GO:1901536; P:negative regulation of DNA demethylation; IMP:UniProtKB.
DR GO; GO:0044726; P:protection of DNA demethylation of female pronucleus; IMP:UniProtKB.
DR GO; GO:2000653; P:regulation of genetic imprinting; IMP:UniProtKB.
DR InterPro; IPR029096; Dppa3.
DR PANTHER; PTHR31577; PTHR31577; 1.
DR Pfam; PF15549; PGC7_Stella; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Cytoplasm; Developmental protein; Nucleus;
KW Reference proteome.
FT CHAIN 1..150
FT /note="Developmental pluripotency-associated protein 3"
FT /id="PRO_0000239266"
FT REGION 1..75
FT /note="Required for H3K9me2-binding"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..150
FT /note="Required to exclude TET3 from the maternal
FT pronucleus"
FT COMPBIAS 1..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 44
FT /note="L->A: Abolishes localization to the nucleus and
FT ability to prevent DNA demethylation; when associated with
FT A-46."
FT /evidence="ECO:0000269|PubMed:17143267"
FT MUTAGEN 46
FT /note="L->A: Abolishes localization to the nucleus and
FT ability to prevent DNA demethylation; when associated with
FT A-44."
FT /evidence="ECO:0000269|PubMed:17143267"
SQ SEQUENCE 150 AA; 17670 MW; 88B59E272F8FAD42 CRC64;
MEEPSEKVDP MKDPETPQKK DEEDALDDTD VLQPETLVKV MKKLTLNPGV KRSARRRSLR
NRIAAVPVEN KSEKIRREVQ SAFPKRRVRT LLSVLKDPIA KMRRLVRIEQ RQKRLEGNEF
ERDSEPFRCL CTFCHYQRWD PSENAKIGKN
