ID   DPPA3_RAT               Reviewed;         158 AA.
AC   Q6IMK0;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Developmental pluripotency-associated protein 3;
GN   Name=Dppa3; Synonyms=Stella;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=14654002; DOI=10.1016/j.cub.2003.11.026;
RA   Payer B., Saitou M., Barton S.C., Thresher R., Dixon J.P., Zahn D.,
RA   Colledge W.H., Carlton M.B., Nakano T., Surani M.A.;
RT   "Stella is a maternal effect gene required for normal early development in
RT   mice.";
RL   Curr. Biol. 13:2110-2117(2003).
CC   -!- FUNCTION: Primordial germ cell (PGCs)-specific protein involved in
CC       epigenetic chromatin reprogramming in the zygote following
CC       fertilization. In zygotes, DNA demethylation occurs selectively in the
CC       paternal pronucleus before the first cell division, while the adjacent
CC       maternal pronucleus and certain paternally-imprinted loci are protected
CC       from this process. Participates in protection of DNA methylation in the
CC       maternal pronucleus by preventing conversion of 5mC to 5hmC:
CC       specifically recognizes and binds histone H3 dimethylated at 'Lys-9'
CC       (H3K9me2) on maternal genome, and protects maternal genome from TET3-
CC       mediated conversion to 5hmC and subsequent DNA demethylation. Does not
CC       bind paternal chromatin, which is mainly packed into protamine and does
CC       not contain much H3K9me2 mark. Also protects imprinted loci that are
CC       marked with H3K9me2 in mature sperm from DNA demethylation in early
CC       embryogenesis. May be important for the totipotent/pluripotent states
CC       continuing through preimplantation development. Also involved in
CC       chromatin condensation in oocytogenesis (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Note=Mainly localizes in the female pronucleus, localization to the
CC       male pronucleus in much weaker. {ECO:0000250}.
CC   -!- DOMAIN: Mediates binding to H3K9me2 via N-terminal region, while
CC       ability to exclude TET3 from the maternal pronucleus requires the C-
CC       terminal part. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BK001414; DAA01452.1; -; mRNA.
DR   RefSeq; NP_001041329.1; NM_001047864.1.
DR   AlphaFoldDB; Q6IMK0; -.
DR   STRING; 10116.ENSRNOP00000036033; -.
DR   PaxDb; Q6IMK0; -.
DR   PRIDE; Q6IMK0; -.
DR   Ensembl; ENSRNOT00000031353; ENSRNOP00000036033; ENSRNOG00000022950.
DR   Ensembl; ENSRNOT00000049192; ENSRNOP00000090420; ENSRNOG00000031344.
DR   GeneID; 297576; -.
DR   KEGG; rno:297576; -.
DR   UCSC; RGD:1310932; rat.
DR   CTD; 359787; -.
DR   RGD; 1310932; Dppa3.
DR   GeneTree; ENSGT00800000124303; -.
DR   HOGENOM; CLU_107188_1_0_1; -.
DR   InParanoid; Q6IMK0; -.
DR   OMA; GICCKAT; -.
DR   OrthoDB; 1539071at2759; -.
DR   PhylomeDB; Q6IMK0; -.
DR   TreeFam; TF338511; -.
DR   PRO; PR:Q6IMK0; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Bgee; ENSRNOG00000022950; Expressed in ovary and 1 other tissue.
DR   Genevisible; Q6IMK0; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0001939; C:female pronucleus; ISS:UniProtKB.
DR   GO; GO:0001940; C:male pronucleus; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0040016; P:embryonic cleavage; ISO:RGD.
DR   GO; GO:1901536; P:negative regulation of DNA demethylation; ISS:UniProtKB.
DR   GO; GO:0044726; P:protection of DNA demethylation of female pronucleus; ISS:UniProtKB.
DR   GO; GO:2000653; P:regulation of genetic imprinting; ISS:UniProtKB.
DR   InterPro; IPR029096; Dppa3.
DR   PANTHER; PTHR31577; PTHR31577; 1.
DR   Pfam; PF15549; PGC7_Stella; 1.
PE   2: Evidence at transcript level;
KW   Chromatin regulator; Cytoplasm; Developmental protein; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..158
FT                   /note="Developmental pluripotency-associated protein 3"
FT                   /id="PRO_0000239267"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          54..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   158 AA;  18834 MW;  0D7928CE627AF761 CRC64;
     MDEPSEKVDP VVNPETQMYD GSQREDEGDS PDDSEILQPE TLVKVMKKLT LNPSAKPTKY
     HRRQRVRLQV KSQPVENRSE RIMREVQSAF PRRRVRTLLS VLKDPIARMR RFVRIEQRQR
     QLEGNERRDE PFRCLCTFCH YQRWDPSENA KIGQNQKN