DPPA4_HUMAN
ID DPPA4_HUMAN Reviewed; 304 AA.
AC Q7L190; A8K4M7; Q9H9N5; Q9NVI6;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Developmental pluripotency-associated protein 4;
GN Name=DPPA4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-55.
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-55.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-215; SER-221 AND SER-226, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH PCGF1.
RX PubMed=26687479; DOI=10.1038/srep18388;
RA Oliviero G., Munawar N., Watson A., Streubel G., Manning G., Bardwell V.,
RA Bracken A.P., Cagney G.;
RT "The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a
RT pluripotency sub-network that includes DPPA4, a regulator of
RT embryogenesis.";
RL Sci. Rep. 5:18388-18388(2015).
CC -!- FUNCTION: May be involved in the maintenance of active epigenetic
CC status of target genes. May inhibit differentiation of embryonic cells
CC into a primitive ectoderm lineage. {ECO:0000250|UniProtKB:Q8CCG4}.
CC -!- SUBUNIT: Interacts with DPPA2 (By similarity). Interacts with PCGF1
CC (PubMed:26687479). {ECO:0000250|UniProtKB:Q8CCG4,
CC ECO:0000269|PubMed:26687479}.
CC -!- INTERACTION:
CC Q7L190; Q86X95-2: CIR1; NbExp=3; IntAct=EBI-710457, EBI-12034850;
CC Q7L190; P46108: CRK; NbExp=5; IntAct=EBI-710457, EBI-886;
CC Q7L190; Q96JC9: EAF1; NbExp=3; IntAct=EBI-710457, EBI-769261;
CC Q7L190; Q14240: EIF4A2; NbExp=3; IntAct=EBI-710457, EBI-73473;
CC Q7L190; Q14240-2: EIF4A2; NbExp=3; IntAct=EBI-710457, EBI-10232522;
CC Q7L190; Q6PIV2: FOXR1; NbExp=3; IntAct=EBI-710457, EBI-10253815;
CC Q7L190; Q9Y5P6: GMPPB; NbExp=3; IntAct=EBI-710457, EBI-750945;
CC Q7L190; Q13588: GRAP; NbExp=3; IntAct=EBI-710457, EBI-2847510;
CC Q7L190; P62993: GRB2; NbExp=3; IntAct=EBI-710457, EBI-401755;
CC Q7L190; Q969Y2: GTPBP3; NbExp=3; IntAct=EBI-710457, EBI-740290;
CC Q7L190; Q9C086: INO80B; NbExp=3; IntAct=EBI-710457, EBI-715611;
CC Q7L190; Q9H8E8: KAT14; NbExp=3; IntAct=EBI-710457, EBI-750907;
CC Q7L190; O95751: LDOC1; NbExp=7; IntAct=EBI-710457, EBI-740738;
CC Q7L190; P61326: MAGOH; NbExp=3; IntAct=EBI-710457, EBI-299134;
CC Q7L190; Q8IVT2: MISP; NbExp=3; IntAct=EBI-710457, EBI-2555085;
CC Q7L190; Q14511-2: NEDD9; NbExp=3; IntAct=EBI-710457, EBI-11746523;
CC Q7L190; Q96KN7: RPGRIP1; NbExp=3; IntAct=EBI-710457, EBI-1050213;
CC Q7L190; Q6FGM0: SH3GL1; NbExp=5; IntAct=EBI-710457, EBI-10173690;
CC Q7L190; Q99961: SH3GL1; NbExp=9; IntAct=EBI-710457, EBI-697911;
CC Q7L190; Q96RF0: SNX18; NbExp=3; IntAct=EBI-710457, EBI-298169;
CC Q7L190; Q8WV41: SNX33; NbExp=5; IntAct=EBI-710457, EBI-2481535;
CC Q7L190; Q5MJ10: SPANXN2; NbExp=3; IntAct=EBI-710457, EBI-12023934;
CC Q7L190; Q9UKI8: TLK1; NbExp=3; IntAct=EBI-710457, EBI-740492;
CC Q7L190; Q15025: TNIP1; NbExp=3; IntAct=EBI-710457, EBI-357849;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CCG4}.
CC Note=Associated with transcriptionally active chromatin.
CC {ECO:0000250|UniProtKB:Q8CCG4}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA91765.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB14188.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK001575; BAA91765.1; ALT_INIT; mRNA.
DR EMBL; AK022698; BAB14188.1; ALT_FRAME; mRNA.
DR EMBL; AK290992; BAF83681.1; -; mRNA.
DR EMBL; AC076971; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC032846; AAH32846.2; -; mRNA.
DR CCDS; CCDS33814.1; -.
DR RefSeq; NP_060659.3; NM_018189.3.
DR AlphaFoldDB; Q7L190; -.
DR BioGRID; 120507; 139.
DR IntAct; Q7L190; 53.
DR MINT; Q7L190; -.
DR STRING; 9606.ENSP00000335306; -.
DR iPTMnet; Q7L190; -.
DR PhosphoSitePlus; Q7L190; -.
DR BioMuta; DPPA4; -.
DR DMDM; 296434484; -.
DR EPD; Q7L190; -.
DR jPOST; Q7L190; -.
DR MassIVE; Q7L190; -.
DR PaxDb; Q7L190; -.
DR PeptideAtlas; Q7L190; -.
DR PRIDE; Q7L190; -.
DR ProteomicsDB; 68742; -.
DR Antibodypedia; 32419; 340 antibodies from 31 providers.
DR DNASU; 55211; -.
DR Ensembl; ENST00000335658.7; ENSP00000335306.6; ENSG00000121570.13.
DR GeneID; 55211; -.
DR KEGG; hsa:55211; -.
DR MANE-Select; ENST00000335658.7; ENSP00000335306.6; NM_018189.4; NP_060659.3.
DR UCSC; uc003dxq.4; human.
DR CTD; 55211; -.
DR DisGeNET; 55211; -.
DR GeneCards; DPPA4; -.
DR HGNC; HGNC:19200; DPPA4.
DR HPA; ENSG00000121570; Tissue enhanced (bone marrow, lymphoid tissue, testis).
DR MIM; 614125; gene.
DR neXtProt; NX_Q7L190; -.
DR OpenTargets; ENSG00000121570; -.
DR PharmGKB; PA134958838; -.
DR VEuPathDB; HostDB:ENSG00000121570; -.
DR eggNOG; ENOG502R0CF; Eukaryota.
DR GeneTree; ENSGT00390000004871; -.
DR HOGENOM; CLU_080062_0_0_1; -.
DR InParanoid; Q7L190; -.
DR OMA; NKVLCPK; -.
DR OrthoDB; 1295613at2759; -.
DR PhylomeDB; Q7L190; -.
DR TreeFam; TF338129; -.
DR PathwayCommons; Q7L190; -.
DR Reactome; R-HSA-2892247; POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation.
DR SignaLink; Q7L190; -.
DR SIGNOR; Q7L190; -.
DR BioGRID-ORCS; 55211; 8 hits in 1061 CRISPR screens.
DR ChiTaRS; DPPA4; human.
DR GenomeRNAi; 55211; -.
DR Pharos; Q7L190; Tbio.
DR PRO; PR:Q7L190; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q7L190; protein.
DR Bgee; ENSG00000121570; Expressed in buccal mucosa cell and 104 other tissues.
DR ExpressionAtlas; Q7L190; baseline and differential.
DR Genevisible; Q7L190; HS.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0060484; P:lung-associated mesenchyme development; IEA:Ensembl.
DR GO; GO:0048731; P:system development; IBA:GO_Central.
DR InterPro; IPR039590; Dppa2/4.
DR InterPro; IPR025891; Dppa2/4_C_dom.
DR InterPro; IPR025892; Dppa2/4_central_dom.
DR InterPro; IPR039836; Dppa4.
DR PANTHER; PTHR16073; PTHR16073; 1.
DR PANTHER; PTHR16073:SF8; PTHR16073:SF8; 1.
DR Pfam; PF14047; DCR; 1.
DR Pfam; PF14049; Dppa2_A; 2.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..304
FT /note="Developmental pluripotency-associated protein 4"
FT /id="PRO_0000239268"
FT REGION 1..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..75
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 215
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 221
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 226
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT VARIANT 55
FT /note="I -> V (in dbSNP:rs3762648)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_060372"
FT VARIANT 226
FT /note="S -> F (in dbSNP:rs3905734)"
FT /id="VAR_060373"
SQ SEQUENCE 304 AA; 33541 MW; EF7C64D23CF2604F CRC64;
MLRGSASSTS MEKAKGKEWT STEKSREEDQ QASNQPNSIA LPGTSAKRTK EKMSIKGSKV
LCPKKKAEHT DNPRPQKKIP IPPLPSKLPP VNLIHRDILR AWCQQLKLSS KGQKLDAYKR
LCAFAYPNQK DFPSTAKEAK IRKSLQKKLK VEKGETSLQS SETHPPEVAL PPVGEPPALE
NSTALLEGVN TVVVTTSAPE ALLASWARIS ARARTPEAVE SPQEASGVRW CVVHGKSLPA
DTDGWVHLQF HAGQAWVPEK QEGRVSALFL LPASNFPPPH LEDNMLCPKC VHRNKVLIKS
LQWE
