DPPA4_MOUSE
ID DPPA4_MOUSE Reviewed; 296 AA.
AC Q8CCG4; Q6P228; Q810Y6;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Developmental pluripotency-associated protein 4;
DE AltName: Full=Embryonic stem cell-associated transcript 15-1 protein;
DE Short=ECAT15-1;
GN Name=Dppa4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=12620990; DOI=10.1242/dev.00366;
RA Bortvin A., Eggan K., Skaletsky H., Akutsu H., Berry D.L., Yanagimachi R.,
RA Page D.C., Jaenisch R.;
RT "Incomplete reactivation of Oct4-related genes in mouse embryos cloned from
RT somatic nuclei.";
RL Development 130:1673-1680(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=B5/EGFP; TISSUE=Trophoblast stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=17855347; DOI=10.1074/jbc.m703245200;
RA Masaki H., Nishida T., Kitajima S., Asahina K., Teraoka H.;
RT "Developmental pluripotency-associated 4 (DPPA4) localized in active
RT chromatin inhibits mouse embryonic stem cell differentiation into a
RT primitive ectoderm lineage.";
RL J. Biol. Chem. 282:33034-33042(2007).
RN [5]
RP FUNCTION, INTERACTION WITH DPPA2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21896782; DOI=10.1128/mcb.05701-11;
RA Nakamura T., Nakagawa M., Ichisaka T., Shiota A., Yamanaka S.;
RT "Essential roles of ECAT15-2/Dppa2 in functional lung development.";
RL Mol. Cell. Biol. 31:4366-4378(2011).
CC -!- FUNCTION: May be involved in the maintenance of active epigenetic
CC status of target genes. May inhibit differentiation of embryonic stem
CC (ES) cells into a primitive ectoderm lineage.
CC {ECO:0000269|PubMed:17855347, ECO:0000269|PubMed:21896782}.
CC -!- SUBUNIT: Interacts with DPPA2 (PubMed:21896782). Interacts with PCGF1
CC (By similarity). {ECO:0000250|UniProtKB:Q7L190,
CC ECO:0000269|PubMed:21896782}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17855347,
CC ECO:0000269|PubMed:21896782}. Note=Associated with transcriptionally
CC active chromatin.
CC -!- TISSUE SPECIFICITY: Expressed in pluripotent embryonic cells, but not
CC in differentiated somatic tissues. {ECO:0000269|PubMed:12620990,
CC ECO:0000269|PubMed:17855347, ECO:0000269|PubMed:21896782}.
CC -!- DEVELOPMENTAL STAGE: Expressed in 4-cell embryo, morula and blastocyst.
CC At 7.5 dpc, detected in the epiblast. At 10.0 dpc, expressed in the
CC primordial germ cells and at 12.0 dpc in the embryonic gonads. At 14.5
CC dpc, expression is restricted to testis. 6 days after birth, still
CC detected in primitive type A spermatogonia. Expressed in
CC undifferentiated embryonic stem cells, but expression decreases upon
CC differentiation (at protein level). {ECO:0000269|PubMed:12620990,
CC ECO:0000269|PubMed:17855347, ECO:0000269|PubMed:21896782}.
CC -!- DISRUPTION PHENOTYPE: The mortality of mutant animals is increased at
CC birth and most die within 3 days. The few surviving neonates show
CC growth retardation, but they catch up with wild-type mice by 20 weeks
CC of age. {ECO:0000269|PubMed:21896782}.
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DR EMBL; AF490348; AAO84506.1; -; mRNA.
DR EMBL; AK033197; BAC28194.1; -; mRNA.
DR EMBL; BC064754; AAH64754.1; -; mRNA.
DR CCDS; CCDS28209.1; -.
DR RefSeq; NP_001018002.1; NM_001018002.1.
DR RefSeq; NP_082886.2; NM_028610.2.
DR AlphaFoldDB; Q8CCG4; -.
DR BioGRID; 216194; 1.
DR STRING; 10090.ENSMUSP00000093749; -.
DR iPTMnet; Q8CCG4; -.
DR PhosphoSitePlus; Q8CCG4; -.
DR MaxQB; Q8CCG4; -.
DR PaxDb; Q8CCG4; -.
DR PeptideAtlas; Q8CCG4; -.
DR PRIDE; Q8CCG4; -.
DR ProteomicsDB; 277604; -.
DR Antibodypedia; 32419; 340 antibodies from 31 providers.
DR DNASU; 73693; -.
DR Ensembl; ENSMUST00000096045; ENSMUSP00000093749; ENSMUSG00000058550.
DR GeneID; 73693; -.
DR KEGG; mmu:73693; -.
DR UCSC; uc007zjl.1; mouse.
DR CTD; 55211; -.
DR MGI; MGI:2157525; Dppa4.
DR VEuPathDB; HostDB:ENSMUSG00000058550; -.
DR eggNOG; ENOG502R0CF; Eukaryota.
DR GeneTree; ENSGT00390000004871; -.
DR HOGENOM; CLU_080062_0_0_1; -.
DR InParanoid; Q8CCG4; -.
DR OMA; NKVLCPK; -.
DR OrthoDB; 1295613at2759; -.
DR PhylomeDB; Q8CCG4; -.
DR TreeFam; TF338129; -.
DR BioGRID-ORCS; 73693; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Dppa4; mouse.
DR PRO; PR:Q8CCG4; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8CCG4; protein.
DR Bgee; ENSMUSG00000058550; Expressed in cleaving embryo and 41 other tissues.
DR ExpressionAtlas; Q8CCG4; baseline and differential.
DR Genevisible; Q8CCG4; MM.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0060484; P:lung-associated mesenchyme development; IMP:MGI.
DR GO; GO:0048731; P:system development; IBA:GO_Central.
DR InterPro; IPR039590; Dppa2/4.
DR InterPro; IPR025891; Dppa2/4_C_dom.
DR InterPro; IPR039836; Dppa4.
DR PANTHER; PTHR16073; PTHR16073; 1.
DR PANTHER; PTHR16073:SF8; PTHR16073:SF8; 1.
DR Pfam; PF14047; DCR; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..296
FT /note="Developmental pluripotency-associated protein 4"
FT /id="PRO_0000239269"
FT REGION 1..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 27
FT /note="S -> G (in Ref. 3; AAH64754)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="K -> R (in Ref. 3; AAH64754)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="S -> R (in Ref. 1; AAO84506)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="M -> L (in Ref. 1; AAO84506)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="D -> Y (in Ref. 1; AAO84506)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="M -> L (in Ref. 1; AAO84506)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="E -> K (in Ref. 2; BAC28194)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 296 AA; 32699 MW; 31CADF37547975B2 CRC64;
METAGDKKWS AEEPKEEVEL QMSSQPSTAP AKAKATGKKQ KKSETDNGCK PKEGKPQDTE
TPGQTRRKVP IPPIPEYLPP VNLIHRDVLR AWCQKKRVSS KGQKLDAYKR LLARAFPEQM
LELRNVPDSA KDARLKTAHK KMKTEPGEES EVTVPLEMVP VPEEQIPALI DPPMLYEEVS
TTVVTTPATE AVLASWARIA SNAKKYEAVP ADASSSSEVK GEMWCVVHGT SLPGNSRGWV
RLQFHAGQAW VPDKKGKAIA LFLLPACTFP PPHLEDNMLC PKCVHKNKIL TKSLEG
