ID   DPRA_HAEIN              Reviewed;         373 AA.
AC   P43862;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=DNA-processing protein A;
DE   AltName: Full=Protein Smf;
GN   Name=dprA {ECO:0000303|PubMed:7768823}; Synonyms=smf;
GN   OrderedLocusNames=HI_0985;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7768823; DOI=10.1128/jb.177.11.3235-3240.1995;
RA   Karudapuram S., Zhao X., Barcak G.J.;
RT   "DNA sequence and characterization of Haemophilus influenzae dprA+, a gene
RT   required for chromosomal but not plasmid DNA transformation.";
RL   J. Bacteriol. 177:3235-3240(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=16978360; DOI=10.1111/j.1574-6968.2006.00423.x;
RA   Smeets L.C., Becker S.C., Barcak G.J., Vandenbroucke-Grauls C.M.,
RA   Bitter W., Goosen N.;
RT   "Functional characterization of the competence protein DprA/Smf in
RT   Escherichia coli.";
RL   FEMS Microbiol. Lett. 263:223-228(2006).
CC   -!- FUNCTION: Essential for efficient chromosomal DNA transformation but
CC       not required for plasmid transformation (PubMed:7768823). Its
CC       disruption can be partially complemented by the smf gene of E.coli
CC       (PubMed:16978360). {ECO:0000269|PubMed:16978360,
CC       ECO:0000269|PubMed:7768823}.
CC   -!- DISRUPTION PHENOTYPE: 2,000-fold decrease in transformation efficiency
CC       (PubMed:7768823, PubMed:16978360). Cells take up DNA but are defective
CC       in a later stage of chromosomal DNA transformation (PubMed:7768823).
CC       {ECO:0000269|PubMed:16978360}.
CC   -!- SIMILARITY: Belongs to the DprA/Smf family. {ECO:0000305}.
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DR   EMBL; U18657; AAA70111.1; -; Genomic_DNA.
DR   EMBL; L42023; AAC22646.1; -; Genomic_DNA.
DR   PIR; D64106; D64106.
DR   RefSeq; NP_439148.1; NC_000907.1.
DR   RefSeq; WP_005693325.1; NC_000907.1.
DR   AlphaFoldDB; P43862; -.
DR   SMR; P43862; -.
DR   STRING; 71421.HI_0985; -.
DR   EnsemblBacteria; AAC22646; AAC22646; HI_0985.
DR   KEGG; hin:HI_0985; -.
DR   PATRIC; fig|71421.8.peg.1028; -.
DR   eggNOG; COG0758; Bacteria.
DR   HOGENOM; CLU_029601_0_3_6; -.
DR   OMA; SEMPFGW; -.
DR   PhylomeDB; P43862; -.
DR   BioCyc; HINF71421:G1GJ1-1027-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0009294; P:DNA-mediated transformation; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003488; DprA.
DR   InterPro; IPR041614; DprA_WH.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR43022; PTHR43022; 1.
DR   Pfam; PF02481; DNA_processg_A; 1.
DR   Pfam; PF17782; DprA_WH; 1.
DR   TIGRFAMs; TIGR00732; dprA; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..373
FT                   /note="DNA-processing protein A"
FT                   /id="PRO_0000209158"
SQ   SEQUENCE   373 AA;  41640 MW;  26E0335372EFE61F CRC64;
     MNDITYTLLR LMQVPKLGGV GIDKILSNIT LNELLNYDDV AFRQMGWGAI QIRRWFKPEA
     KFIEPALVWS QKEGNHLVNY FSPFYPFLLK QTASFPPLLF VKGNLTALSQ RQMAMVGSRY
     CTTYGEYWAK HFATELSLAG FTITSGLALG IDGHCHQAVV NIQGQTIAVL GSGLEQIYPS
     KHQRLSAQII ENNGALVSEF LPNQAPIAAN FPRRNRIISG LSVGTLVVEA TEKSGSLITA
     RYALEQNREV FAVPGNIQNK SSQGCHRLIK QGAMLVENAK DILETLYQHS IHSQTEIDFD
     QIAVPNYTPP PDPRRLVEAP SHPKLYSRIG YTPVSIDDLA EEFNLSVDVL LVQLLDLELQ
     DLIISENGLY KRV