ADEQ_ECOLI
ID ADEQ_ECOLI Reviewed; 444 AA.
AC P31440; P76726; Q2M7X7;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Adenine permease AdeQ;
GN Name=adeQ; Synonyms=yicO; OrderedLocusNames=b3664, JW5636;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND GENE
RP NAME.
RC STRAIN=K12;
RX PubMed=24214977; DOI=10.1074/jbc.m113.523340;
RA Papakostas K., Botou M., Frillingos S.;
RT "Functional identification of the hypoxanthine/guanine transporters YjcD
RT and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-
RT 12.";
RL J. Biol. Chem. 288:36827-36840(2013).
CC -!- FUNCTION: High-affinity transporter for adenine.
CC {ECO:0000269|PubMed:24214977}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6.5 uM for adenine {ECO:0000269|PubMed:24214977};
CC Vmax=11.0 nmol/min/mg enzyme {ECO:0000269|PubMed:24214977};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:24214977}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:24214977}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. Azg-like subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA62016.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L10328; AAA62016.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76687.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77629.1; -; Genomic_DNA.
DR PIR; A65168; A65168.
DR RefSeq; NP_418120.2; NC_000913.3.
DR RefSeq; WP_001403836.1; NZ_SSZK01000043.1.
DR AlphaFoldDB; P31440; -.
DR BioGRID; 4260893; 115.
DR IntAct; P31440; 1.
DR STRING; 511145.b3664; -.
DR TCDB; 2.A.40.7.4; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
DR PaxDb; P31440; -.
DR PRIDE; P31440; -.
DR DNASU; 948174; -.
DR EnsemblBacteria; AAC76687; AAC76687; b3664.
DR EnsemblBacteria; BAE77629; BAE77629; BAE77629.
DR GeneID; 948174; -.
DR KEGG; ecj:JW5636; -.
DR KEGG; eco:b3664; -.
DR PATRIC; fig|511145.12.peg.3786; -.
DR EchoBASE; EB1642; -.
DR eggNOG; COG2252; Bacteria.
DR HOGENOM; CLU_024508_0_1_6; -.
DR InParanoid; P31440; -.
DR OMA; YVDIIDC; -.
DR PhylomeDB; P31440; -.
DR BioCyc; EcoCyc:EG11691-MON; -.
DR BioCyc; MetaCyc:EG11691-MON; -.
DR SABIO-RK; P31440; -.
DR PRO; PR:P31440; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015207; F:adenine transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015853; P:adenine transport; IMP:EcoCyc.
DR InterPro; IPR045018; Azg-like.
DR InterPro; IPR026033; Azg-like_bact_archaea.
DR InterPro; IPR006043; NCS2.
DR PANTHER; PTHR43337; PTHR43337; 1.
DR Pfam; PF00860; Xan_ur_permease; 1.
DR PIRSF; PIRSF005353; PbuG; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..444
FT /note="Adenine permease AdeQ"
FT /id="PRO_0000169621"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..63
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..101
FT /note="Discontinuously helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..113
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..145
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..182
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..251
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..311
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..327
FT /note="Discontinuously helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..357
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..382
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 383..418
FT /note="Discontinuously helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 444 AA; 46934 MW; 555CECFDB53324B6 CRC64;
MNNDNTDYVS NESGTLSRLF KLPQHGTTVR TELIAGMTTF LTMVYIVFVN PQILGAAQMD
PKVVFVTTCL IAGIGSIAMG IFANLPVALA PAMGLNAFFA FVVVGAMGIS WQTGMGAIFW
GAVGLFLLTL FRIRYWMISN IPLSLRIGIT SGIGLFIALM GLKNTGVIVA NKDTLVMIGD
LSSHGVLLGI LGFFIITVLS SRHFHAAVLV SIVVTSCCGL FFGDVHFSGV YSIPPDISGV
IGEVDLSGAL TLELAGIIFS FMLINLFDSS GTLIGVTDKA GLIDGNGKFP NMNKALYVDS
VSSVAGAFIG TSSVTAYIES TSGVAVGGRT GLTAVVVGVM FLLVMFFSPL VAIVPPYATA
GALIFVGVLM TSSLARVNWD DFTESVPAFI TTVMMPFTFS ITEGIALGFM SYCIMKVCTG
RWRDLNLCVV VVAALFALKI ILVD
