DRAM1_HUMAN
ID DRAM1_HUMAN Reviewed; 238 AA.
AC Q8N682; B7Z4T0; Q7L3E3; Q9NUN1;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA damage-regulated autophagy modulator protein 1;
DE AltName: Full=Damage-regulated autophagy modulator;
GN Name=DRAM1; Synonyms=DRAM;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 78-238 (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Liver, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=16839881; DOI=10.1016/j.cell.2006.05.034;
RA Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P., Harrison P.R.,
RA Gasco M., Garrone O., Crook T., Ryan K.M.;
RT "DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.";
RL Cell 126:121-134(2006).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=17304243; DOI=10.1038/sj.cdd.4402108;
RA Crighton D., O'prey J., Bell H.S., Ryan K.M.;
RT "p73 regulates DRAM-independent autophagy that does not contribute to
RT programmed cell death.";
RL Cell Death Differ. 14:1071-1079(2007).
CC -!- FUNCTION: Lysosomal modulator of autophagy that plays a central role in
CC p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated
CC autophagy. {ECO:0000269|PubMed:16839881, ECO:0000269|PubMed:17304243}.
CC -!- INTERACTION:
CC Q8N682; Q9H246: C1orf21; NbExp=3; IntAct=EBI-10305400, EBI-10305393;
CC Q8N682; Q6UWJ8-2: CD164L2; NbExp=3; IntAct=EBI-10305400, EBI-13362802;
CC Q8N682; P58418: CLRN1; NbExp=3; IntAct=EBI-10305400, EBI-17274839;
CC Q8N682; P34910-2: EVI2B; NbExp=3; IntAct=EBI-10305400, EBI-17640610;
CC Q8N682; O15529: GPR42; NbExp=3; IntAct=EBI-10305400, EBI-18076404;
CC Q8N682; Q9HCJ2: LRRC4C; NbExp=3; IntAct=EBI-10305400, EBI-3925442;
CC Q8N682; Q9GZW8: MS4A7; NbExp=3; IntAct=EBI-10305400, EBI-721391;
CC Q8N682; Q9NPL8: TIMMDC1; NbExp=3; IntAct=EBI-10305400, EBI-6268651;
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:16839881};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16839881}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N682-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N682-2; Sequence=VSP_056980;
CC -!- INDUCTION: By p53/TP53 and p73/TP73. Directly activated by p53/TP53.
CC Significantly down-regulated in tumor cell lines by methylation-
CC dependent transcriptional silencing. {ECO:0000269|PubMed:16839881,
CC ECO:0000269|PubMed:17304243}.
CC -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA92091.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/DRAMID44093ch12q23.html";
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DR EMBL; AK002121; BAA92091.1; ALT_INIT; mRNA.
DR EMBL; AK297785; BAH12666.1; -; mRNA.
DR EMBL; AC063950; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC079907; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084398; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC013773; AAH13773.4; -; mRNA.
DR EMBL; BC018435; AAH18435.1; -; mRNA.
DR CCDS; CCDS41823.1; -. [Q8N682-1]
DR RefSeq; NP_060840.2; NM_018370.2. [Q8N682-1]
DR AlphaFoldDB; Q8N682; -.
DR SMR; Q8N682; -.
DR BioGRID; 120613; 11.
DR IntAct; Q8N682; 10.
DR STRING; 9606.ENSP00000258534; -.
DR TCDB; 8.A.113.1.8; the tentonin or tmem150 (tmem150) family.
DR iPTMnet; Q8N682; -.
DR PhosphoSitePlus; Q8N682; -.
DR BioMuta; DRAM1; -.
DR DMDM; 74729109; -.
DR MassIVE; Q8N682; -.
DR PaxDb; Q8N682; -.
DR PeptideAtlas; Q8N682; -.
DR PRIDE; Q8N682; -.
DR Antibodypedia; 9122; 295 antibodies from 30 providers.
DR DNASU; 55332; -.
DR Ensembl; ENST00000258534.13; ENSP00000258534.8; ENSG00000136048.14. [Q8N682-1]
DR Ensembl; ENST00000544152.5; ENSP00000445827.1; ENSG00000136048.14. [Q8N682-2]
DR GeneID; 55332; -.
DR KEGG; hsa:55332; -.
DR MANE-Select; ENST00000258534.13; ENSP00000258534.8; NM_018370.3; NP_060840.2.
DR UCSC; uc001tix.4; human. [Q8N682-1]
DR CTD; 55332; -.
DR DisGeNET; 55332; -.
DR GeneCards; DRAM1; -.
DR HGNC; HGNC:25645; DRAM1.
DR HPA; ENSG00000136048; Tissue enhanced (lung).
DR MIM; 610776; gene.
DR neXtProt; NX_Q8N682; -.
DR OpenTargets; ENSG00000136048; -.
DR PharmGKB; PA165512564; -.
DR VEuPathDB; HostDB:ENSG00000136048; -.
DR eggNOG; KOG4320; Eukaryota.
DR GeneTree; ENSGT01030000234578; -.
DR HOGENOM; CLU_059992_2_2_1; -.
DR InParanoid; Q8N682; -.
DR OMA; WYFHVVS; -.
DR PhylomeDB; Q8N682; -.
DR TreeFam; TF314508; -.
DR PathwayCommons; Q8N682; -.
DR SignaLink; Q8N682; -.
DR BioGRID-ORCS; 55332; 15 hits in 1074 CRISPR screens.
DR ChiTaRS; DRAM1; human.
DR GenomeRNAi; 55332; -.
DR Pharos; Q8N682; Tbio.
DR PRO; PR:Q8N682; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q8N682; protein.
DR Bgee; ENSG00000136048; Expressed in lower lobe of lung and 149 other tissues.
DR ExpressionAtlas; Q8N682; baseline and differential.
DR Genevisible; Q8N682; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; IDA:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0010506; P:regulation of autophagy; IDA:UniProtKB.
DR InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR Pfam; PF10277; Frag1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Autophagy; Lysosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..238
FT /note="DNA damage-regulated autophagy modulator protein 1"
FT /id="PRO_0000287436"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 113..222
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056980"
SQ SEQUENCE 238 AA; 26253 MW; 511875677737F6C0 CRC64;
MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP PESGIFGFMI
NFSAFLGAAT MYTRYKIVQK QNQTCYFSTP VFNLVSLVLG LVGCFGMGIV ANFQELAVPV
VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ WNSLSTCHIR MVISAVSCAA VIPMIVCASL
ISITKLEWNP REKDYVYHVV SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINGDI
