DRAM1_MOUSE
ID DRAM1_MOUSE Reviewed; 238 AA.
AC Q9DC58; Q78J26;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=DNA damage-regulated autophagy modulator protein 1;
DE AltName: Full=Damage-regulated autophagy modulator;
GN Name=Dram1; Synonyms=Dram;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Lysosomal modulator of autophagy that plays a central role in
CC p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated
CC autophagy (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9DC58-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DC58-2; Sequence=VSP_025461;
CC -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK004552; BAB23366.2; -; mRNA.
DR EMBL; BC021433; AAH21433.1; -; mRNA.
DR CCDS; CCDS24109.1; -. [Q9DC58-1]
DR RefSeq; NP_082154.2; NM_027878.2. [Q9DC58-1]
DR AlphaFoldDB; Q9DC58; -.
DR SMR; Q9DC58; -.
DR IntAct; Q9DC58; 1.
DR MINT; Q9DC58; -.
DR STRING; 10090.ENSMUSP00000020249; -.
DR PhosphoSitePlus; Q9DC58; -.
DR PaxDb; Q9DC58; -.
DR PRIDE; Q9DC58; -.
DR ProteomicsDB; 279571; -. [Q9DC58-1]
DR ProteomicsDB; 279572; -. [Q9DC58-2]
DR Antibodypedia; 9122; 295 antibodies from 30 providers.
DR DNASU; 71712; -.
DR Ensembl; ENSMUST00000020249; ENSMUSP00000020249; ENSMUSG00000020057. [Q9DC58-1]
DR GeneID; 71712; -.
DR KEGG; mmu:71712; -.
DR UCSC; uc007grj.1; mouse. [Q9DC58-1]
DR CTD; 55332; -.
DR MGI; MGI:1918962; Dram1.
DR VEuPathDB; HostDB:ENSMUSG00000020057; -.
DR eggNOG; KOG4320; Eukaryota.
DR GeneTree; ENSGT01030000234578; -.
DR HOGENOM; CLU_059992_2_2_1; -.
DR InParanoid; Q9DC58; -.
DR OMA; WYFHVVS; -.
DR OrthoDB; 1199230at2759; -.
DR PhylomeDB; Q9DC58; -.
DR TreeFam; TF314508; -.
DR BioGRID-ORCS; 71712; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Dram1; mouse.
DR PRO; PR:Q9DC58; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q9DC58; protein.
DR Bgee; ENSMUSG00000020057; Expressed in left lung lobe and 139 other tissues.
DR ExpressionAtlas; Q9DC58; baseline and differential.
DR Genevisible; Q9DC58; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0010506; P:regulation of autophagy; ISO:MGI.
DR InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR Pfam; PF10277; Frag1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Apoptosis; Autophagy; Lysosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..238
FT /note="DNA damage-regulated autophagy modulator protein 1"
FT /id="PRO_0000287437"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..106
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025461"
SQ SEQUENCE 238 AA; 26201 MW; AC89F1301B0A0048 CRC64;
MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP PESGIFGFMI
NFSAFLGAAT MYTRYKIVEK QNETCYFSTP VFNLVSLALG LVGCIGMGIV ANFQELAVPV
VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ WNSLTTCHVR MAISAVSCAA VVPMIACASL
ISITKLEWNP KEKDYIYHVV SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINDDF
