DRAM2_MOUSE
ID DRAM2_MOUSE Reviewed; 267 AA.
AC Q9CR48; Q9D520;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=DNA damage-regulated autophagy modulator protein 2;
DE AltName: Full=Transmembrane protein 77;
GN Name=Dram2; Synonyms=Tmem77;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Embryo, Mammary gland, Spleen, Testis, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=25983245; DOI=10.1016/j.ajhg.2015.04.006;
RG UK Inherited Retinal Disease Consortium;
RA El-Asrag M.E., Sergouniotis P.I., McKibbin M., Plagnol V., Sheridan E.,
RA Waseem N., Abdelhamed Z., McKeefry D., Van Schil K., Poulter J.A.,
RA Johnson C.A., Carr I.M., Leroy B.P., De Baere E., Inglehearn C.F.,
RA Webster A.R., Toomes C., Ali M.;
RT "Biallelic mutations in the autophagy regulator DRAM2 cause retinal
RT dystrophy with early macular involvement.";
RL Am. J. Hum. Genet. 96:948-954(2015).
CC -!- FUNCTION: Plays a role in the initiation of autophagy. In the retina,
CC might be involved in the process of photoreceptor cells renewal and
CC recycling to preserve visual function. Induces apoptotic cell death
CC when coexpressed with DRAM1. {ECO:0000250|UniProtKB:Q6UX65}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q6UX65};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:Q6UX65}.
CC Photoreceptor inner segment {ECO:0000269|PubMed:25983245}. Apical cell
CC membrane {ECO:0000269|PubMed:25983245}. Note=Localized to photoreceptor
CC inner segments and to the apical surface of retinal pigment epithelial
CC cells. {ECO:0000269|PubMed:25983245}.
CC -!- TISSUE SPECIFICITY: Expressed in the retina.
CC {ECO:0000269|PubMed:25983245}.
CC -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
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DR EMBL; AK009940; BAB26598.1; -; mRNA.
DR EMBL; AK012044; BAB27990.1; -; mRNA.
DR EMBL; AK015888; BAB30020.1; -; mRNA.
DR EMBL; AK052824; BAC35162.1; -; mRNA.
DR EMBL; AK172455; BAE43014.1; -; mRNA.
DR CCDS; CCDS17725.1; -.
DR RefSeq; NP_001273915.1; NM_001286986.1.
DR RefSeq; NP_001273916.1; NM_001286987.1.
DR RefSeq; NP_080289.1; NM_026013.3.
DR AlphaFoldDB; Q9CR48; -.
DR STRING; 10090.ENSMUSP00000063510; -.
DR iPTMnet; Q9CR48; -.
DR PhosphoSitePlus; Q9CR48; -.
DR jPOST; Q9CR48; -.
DR MaxQB; Q9CR48; -.
DR PaxDb; Q9CR48; -.
DR PeptideAtlas; Q9CR48; -.
DR PRIDE; Q9CR48; -.
DR ProteomicsDB; 279484; -.
DR Antibodypedia; 20104; 78 antibodies from 19 providers.
DR DNASU; 67171; -.
DR Ensembl; ENSMUST00000067630; ENSMUSP00000063510; ENSMUSG00000027900.
DR Ensembl; ENSMUST00000121034; ENSMUSP00000112680; ENSMUSG00000027900.
DR GeneID; 67171; -.
DR KEGG; mmu:67171; -.
DR UCSC; uc008qwd.2; mouse.
DR CTD; 128338; -.
DR MGI; MGI:1914421; Dram2.
DR VEuPathDB; HostDB:ENSMUSG00000027900; -.
DR eggNOG; KOG4320; Eukaryota.
DR GeneTree; ENSGT01030000234578; -.
DR HOGENOM; CLU_059992_2_2_1; -.
DR InParanoid; Q9CR48; -.
DR OMA; IYMLVQT; -.
DR OrthoDB; 1199230at2759; -.
DR PhylomeDB; Q9CR48; -.
DR TreeFam; TF314508; -.
DR BioGRID-ORCS; 67171; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Dram2; mouse.
DR PRO; PR:Q9CR48; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9CR48; protein.
DR Bgee; ENSMUSG00000027900; Expressed in intercostal muscle and 256 other tissues.
DR ExpressionAtlas; Q9CR48; baseline and differential.
DR Genevisible; Q9CR48; MM.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0001917; C:photoreceptor inner segment; IDA:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0045494; P:photoreceptor cell maintenance; IMP:UniProtKB.
DR GO; GO:0010506; P:regulation of autophagy; ISO:MGI.
DR GO; GO:0007601; P:visual perception; ISS:UniProtKB.
DR InterPro; IPR032990; DRAM2.
DR InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR PANTHER; PTHR21324:SF10; PTHR21324:SF10; 1.
DR Pfam; PF10277; Frag1; 1.
PE 2: Evidence at transcript level;
KW Apoptosis; Autophagy; Cell membrane; Lysosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..267
FT /note="DNA damage-regulated autophagy modulator protein 2"
FT /id="PRO_0000254103"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 253
FT /note="P -> L (in Ref. 1; BAB30020)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 267 AA; 30228 MW; 228214D5AFF36783 CRC64;
MWWFQQGLSF LPSALVIWTF ATFIFSYITA ITLHHVDPAL PYISDTGTIP PERCLFGVML
NIAAVLGIAT MYVRYKQVHA LNPEENLIIK LNKAGLVLGI LSCLGLSLVA NFQKSTLFIV
HVCGAVLAFS MGSFYMFVQT ILSYQMQPKI HSKQVFWVRL LLVIWCGVSA LSMMTCSSIL
YSSDFGPDVV QKLHWNPEDK GYVLHLVTTA AEWSMSFSFF GFFLTYIRDF QKITLRVEAN
LHGLTLYDTV PCPVNNERTP LLSRDFQ
