ID   DRAM2_RAT               Reviewed;         267 AA.
AC   Q5BK09;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=DNA damage-regulated autophagy modulator protein 2;
DE   AltName: Full=Transmembrane protein 77;
GN   Name=Dram2; Synonyms=Tmem77;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Plays a role in the initiation of autophagy. In the retina,
CC       might be involved in the process of photoreceptor cells renewal and
CC       recycling to preserve visual function. Induces apoptotic cell death
CC       when coexpressed with DRAM1. {ECO:0000250|UniProtKB:Q6UX65}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q6UX65};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:Q6UX65}.
CC       Photoreceptor inner segment {ECO:0000250|UniProtKB:Q9CR48}. Apical cell
CC       membrane {ECO:0000250|UniProtKB:Q9CR48}. Note=Localized to
CC       photoreceptor inner segments and to the apical surface of retinal
CC       pigment epithelial cells. {ECO:0000250|UniProtKB:Q9CR48}.
CC   -!- SIMILARITY: Belongs to the DRAM/TMEM150 family. {ECO:0000305}.
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DR   EMBL; BC091251; AAH91251.1; -; mRNA.
DR   RefSeq; NP_001020189.1; NM_001025018.1.
DR   RefSeq; XP_006233182.1; XM_006233120.3.
DR   RefSeq; XP_006233183.1; XM_006233121.2.
DR   RefSeq; XP_006233184.1; XM_006233122.3.
DR   AlphaFoldDB; Q5BK09; -.
DR   STRING; 10116.ENSRNOP00000023903; -.
DR   PaxDb; Q5BK09; -.
DR   Ensembl; ENSRNOT00000023903; ENSRNOP00000023903; ENSRNOG00000017744.
DR   GeneID; 362011; -.
DR   KEGG; rno:362011; -.
DR   UCSC; RGD:1564099; rat.
DR   CTD; 128338; -.
DR   RGD; 1564099; Dram2.
DR   eggNOG; KOG4320; Eukaryota.
DR   GeneTree; ENSGT01030000234578; -.
DR   HOGENOM; CLU_059992_2_2_1; -.
DR   InParanoid; Q5BK09; -.
DR   OMA; IYMLVQT; -.
DR   OrthoDB; 1199230at2759; -.
DR   PhylomeDB; Q5BK09; -.
DR   TreeFam; TF314508; -.
DR   PRO; PR:Q5BK09; -.
DR   Proteomes; UP000002494; Chromosome 2.
DR   Bgee; ENSRNOG00000017744; Expressed in liver and 19 other tissues.
DR   Genevisible; Q5BK09; RN.
DR   GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0001917; C:photoreceptor inner segment; ISS:UniProtKB.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0045494; P:photoreceptor cell maintenance; ISS:UniProtKB.
DR   GO; GO:0010506; P:regulation of autophagy; ISO:RGD.
DR   GO; GO:0007601; P:visual perception; ISS:UniProtKB.
DR   InterPro; IPR032990; DRAM2.
DR   InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR   PANTHER; PTHR21324:SF10; PTHR21324:SF10; 1.
DR   Pfam; PF10277; Frag1; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Autophagy; Cell membrane; Lysosome; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..267
FT                   /note="DNA damage-regulated autophagy modulator protein 2"
FT                   /id="PRO_0000254104"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   267 AA;  30173 MW;  5B8D99C7A8B018E1 CRC64;
     MWWFQQGLSF LPSALVIWTF ATFIFSYITA ITLHHVDPAL PYISDTGTMP PERCLFGVML
     NIAAVLGIAT IYVRYKQVHA LNPEENLIIK LNKAGLVLGI LSCLGLSLVA NFQKSALFIV
     HVCGAVLAFS MGSFYMFVQT ILSYQMQPKI HSKQVFWVRL LLVIWCGVSA LSMMTCSSIL
     YSSDFGADIV QKLHWNPEDK GYVLHIVTTA AEWSMSFSFF GFFLTYIRDF QKITLRVEAN
     LHGLTLYDTV PCPVTNERTP LLSRDFQ