DRAXI_HUMAN
ID DRAXI_HUMAN Reviewed; 349 AA.
AC Q8NBI3; B6EV15; Q5SNX0; Q6UWK8;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Draxin {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Dorsal inhibitory axon guidance protein {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Dorsal repulsive axon guidance protein {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Neucrin;
DE Flags: Precursor;
GN Name=DRAXIN {ECO:0000255|HAMAP-Rule:MF_03060}; Synonyms=C1orf187;
GN ORFNames=PSEC0258, UNQ3119/PRO10268;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT PHE-37.
RX PubMed=19857465; DOI=10.1016/j.bbrc.2009.10.113;
RA Miyake A., Takahashi Y., Miwa H., Shimada A., Konishi M., Itoh N.;
RT "Neucrin is a novel neural-specific secreted antagonist to canonical Wnt
RT signaling.";
RL Biochem. Biophys. Res. Commun. 390:1051-1055(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PHE-37.
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 26-40, AND VARIANT PHE-37.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
CC -!- FUNCTION: Chemorepulsive axon guidance protein required for the
CC development of spinal cord and forebrain commissures. Acts as a
CC chemorepulsive guidance protein for commissural axons during
CC development. Able to inhibit or repel neurite outgrowth from dorsal
CC spinal cord. Inhibits the stabilization of cytosolic beta-catenin
CC (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist
CC of Wnt signaling pathway. {ECO:0000255|HAMAP-Rule:MF_03060}.
CC -!- SUBUNIT: Interacts with LRP6. {ECO:0000255|HAMAP-Rule:MF_03060}.
CC -!- INTERACTION:
CC Q8NBI3; O95631: NTN1; NbExp=3; IntAct=EBI-10827752, EBI-2678626;
CC Q8NBI3; O00634: NTN3; NbExp=2; IntAct=EBI-10827752, EBI-10831998;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the draxin family. {ECO:0000255|HAMAP-
CC Rule:MF_03060}.
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DR EMBL; AB301919; BAG80561.1; -; mRNA.
DR EMBL; AY358750; AAQ89110.1; -; mRNA.
DR EMBL; AK075558; BAC11697.1; -; mRNA.
DR EMBL; AL953897; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL031731; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC111064; AAI11065.1; -; mRNA.
DR CCDS; CCDS135.1; -.
DR RefSeq; NP_940947.3; NM_198545.3.
DR PDB; 6FKQ; X-ray; 3.07 A; B=225-243.
DR PDBsum; 6FKQ; -.
DR AlphaFoldDB; Q8NBI3; -.
DR SASBDB; Q8NBI3; -.
DR SMR; Q8NBI3; -.
DR IntAct; Q8NBI3; 3.
DR STRING; 9606.ENSP00000294485; -.
DR GlyGen; Q8NBI3; 1 site.
DR iPTMnet; Q8NBI3; -.
DR PhosphoSitePlus; Q8NBI3; -.
DR BioMuta; DRAXIN; -.
DR DMDM; 124007126; -.
DR EPD; Q8NBI3; -.
DR MassIVE; Q8NBI3; -.
DR PaxDb; Q8NBI3; -.
DR PeptideAtlas; Q8NBI3; -.
DR PRIDE; Q8NBI3; -.
DR Antibodypedia; 2659; 144 antibodies from 25 providers.
DR DNASU; 374946; -.
DR Ensembl; ENST00000294485.6; ENSP00000294485.5; ENSG00000162490.7.
DR GeneID; 374946; -.
DR KEGG; hsa:374946; -.
DR MANE-Select; ENST00000294485.6; ENSP00000294485.5; NM_198545.4; NP_940947.3.
DR UCSC; uc001ass.4; human.
DR CTD; 374946; -.
DR DisGeNET; 374946; -.
DR GeneCards; DRAXIN; -.
DR HGNC; HGNC:25054; DRAXIN.
DR HPA; ENSG00000162490; Tissue enriched (brain).
DR MIM; 612682; gene.
DR neXtProt; NX_Q8NBI3; -.
DR OpenTargets; ENSG00000162490; -.
DR PharmGKB; PA142672435; -.
DR VEuPathDB; HostDB:ENSG00000162490; -.
DR eggNOG; ENOG502QUE0; Eukaryota.
DR GeneTree; ENSGT00390000013828; -.
DR HOGENOM; CLU_063473_0_0_1; -.
DR InParanoid; Q8NBI3; -.
DR OMA; NGEQGSF; -.
DR OrthoDB; 860902at2759; -.
DR PhylomeDB; Q8NBI3; -.
DR TreeFam; TF333255; -.
DR PathwayCommons; Q8NBI3; -.
DR SignaLink; Q8NBI3; -.
DR BioGRID-ORCS; 374946; 5 hits in 1061 CRISPR screens.
DR GenomeRNAi; 374946; -.
DR Pharos; Q8NBI3; Tbio.
DR PRO; PR:Q8NBI3; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q8NBI3; protein.
DR Bgee; ENSG00000162490; Expressed in cortical plate and 44 other tissues.
DR Genevisible; Q8NBI3; HS.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0021960; P:anterior commissure morphogenesis; IEA:Ensembl.
DR GO; GO:0007411; P:axon guidance; ISS:UniProtKB.
DR GO; GO:0021528; P:commissural neuron differentiation in spinal cord; ISS:UniProtKB.
DR GO; GO:0021516; P:dorsal spinal cord development; ISS:UniProtKB.
DR GO; GO:0030900; P:forebrain development; ISS:UniProtKB.
DR GO; GO:0110088; P:hippocampal neuron apoptotic process; IEA:Ensembl.
DR GO; GO:0030517; P:negative regulation of axon extension; IEA:Ensembl.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; ISS:UniProtKB.
DR GO; GO:0110091; P:negative regulation of hippocampal neuron apoptotic process; IEA:Ensembl.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR HAMAP; MF_03060; Draxin; 1.
DR InterPro; IPR029094; Draxin.
DR PANTHER; PTHR28610; PTHR28610; 1.
DR Pfam; PF15550; Draxin; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; Direct protein sequencing;
KW Glycoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..25
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03060,
FT ECO:0000269|PubMed:15340161"
FT CHAIN 26..349
FT /note="Draxin"
FT /id="PRO_0000273243"
FT REGION 38..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..261
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03060"
FT VARIANT 37
FT /note="L -> F (in dbSNP:rs11121804)"
FT /evidence="ECO:0000269|PubMed:15340161,
FT ECO:0000269|PubMed:16303743, ECO:0000269|PubMed:19857465"
FT /id="VAR_030114"
FT CONFLICT 20..21
FT /note="LE -> Q (in Ref. 1; AAQ89110)"
FT /evidence="ECO:0000305"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:6FKQ"
FT HELIX 239..241
FT /evidence="ECO:0007829|PDB:6FKQ"
SQ SEQUENCE 349 AA; 38650 MW; 8AC00A42A1AFB105 CRC64;
MAGPAIHTAP MLFLVLLLPL ELSLAGALAP GTPARNLPEN HIDLPGPALW TPQASHHRRR
GPGKKEWGPG LPSQAQDGAV VTATRQASRL PEAEGLLPEQ SPAGLLQDKD LLLGLALPYP
EKENRPPGWE RTRKRSREHK RRRDRLRLHQ GRALVRGPSS LMKKAELSEA QVLDAAMEES
STSLAPTMFF LTTFEAAPAT EESLILPVTS LRPQQAQPRS DGEVMPTLDM ALFDWTDYED
LKPDGWPSAK KKEKHRGKLS SDGNETSPAE GEPCDHHQDC LPGTCCDLRE HLCTPHNRGL
NNKCFDDCMC VEGLRCYAKF HRNRRVTRRK GRCVEPETAN GDQGSFINV
