DRAXI_MOUSE
ID DRAXI_MOUSE Reviewed; 343 AA.
AC Q6PAL1; A2A7G4; B2KFL9; Q8C3D8;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Draxin {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Dorsal inhibitory axon guidance protein {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Dorsal repulsive axon guidance protein {ECO:0000255|HAMAP-Rule:MF_03060};
DE AltName: Full=Neucrin;
DE Flags: Precursor;
GN Name=Draxin;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH LRP6, AND TISSUE
RP SPECIFICITY.
RX PubMed=19857465; DOI=10.1016/j.bbrc.2009.10.113;
RA Miyake A., Takahashi Y., Miwa H., Shimada A., Konishi M., Itoh N.;
RT "Neucrin is a novel neural-specific secreted antagonist to canonical Wnt
RT signaling.";
RL Biochem. Biophys. Res. Commun. 390:1051-1055(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19150847; DOI=10.1126/science.1165187;
RA Islam S.M., Shinmyo Y., Okafuji T., Su Y., Naser I.B., Ahmed G., Zhang S.,
RA Chen S., Ohta K., Kiyonari H., Abe T., Tanaka S., Nishinakamura R.,
RA Terashima T., Kitamura T., Tanaka H.;
RT "Draxin, a repulsive guidance protein for spinal cord and forebrain
RT commissures.";
RL Science 323:388-393(2009).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23206892; DOI=10.1016/j.ydbio.2012.11.029;
RA Hossain M., Ahmed G., Naser I.B., Shinmyo Y., Ito A., Riyadh M.A.,
RA Felemban A., Song X., Ohta K., Tanaka H.;
RT "The combinatorial guidance activities of draxin and Tsukushi are essential
RT for forebrain commissure formation.";
RL Dev. Biol. 374:58-70(2013).
CC -!- FUNCTION: Chemorepulsive axon guidance protein required for the
CC development of spinal cord and forebrain commissures. Acts as a
CC chemorepulsive guidance protein for commissural axons during
CC development. Able to inhibit or repel neurite outgrowth from dorsal
CC spinal cord. Inhibits the stabilization of cytosolic beta-catenin
CC (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist
CC of Wnt signaling pathway. {ECO:0000255|HAMAP-Rule:MF_03060,
CC ECO:0000269|PubMed:19857465, ECO:0000269|PubMed:23206892}.
CC -!- SUBUNIT: Interacts with LRP6. {ECO:0000255|HAMAP-Rule:MF_03060,
CC ECO:0000269|PubMed:19857465}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_03060}.
CC -!- TISSUE SPECIFICITY: In embryonic brain, strong expression in the
CC olfactory bulb, anterior olfactory nucleus, neocortex, piriform cortex,
CC glial wedge, midline zipper glia, indusium griseum and the area
CC surrounding the anterior commissure (AC) but not on AC axons (at
CC protein level) (PubMed:23206892). Predominantly expressed in developing
CC neural tissues. Expressed in many brain regions, including the
CC olfactory bulb, cortex, midbrain, cerebellum and pontine nuclei in
CC neonates. Detected in the dorsal spinal cord and commissural axons. In
CC the forebrain commissures, it is expressed in the regions that surround
CC the corpus callosum, hippocampal commissure, and anterior commissure,
CC such as the midline glial cells, indusium griseum glia, and glial
CC wedge. {ECO:0000269|PubMed:19150847, ECO:0000269|PubMed:19857465,
CC ECO:0000269|PubMed:23206892}.
CC -!- DISRUPTION PHENOTYPE: Mice are viable and fertile (PubMed:19150847).
CC They however show a defasciculation of spinal cord commissural axons
CC and absence of all forebrain commissures (PubMed:19150847,
CC PubMed:23206892). Double knockout of Tsku and Draxi results in a higher
CC frequency of anterior commissure defects than single knockout of either
CC Tsku or Draxi (PubMed:23206892). {ECO:0000269|PubMed:19150847,
CC ECO:0000269|PubMed:23206892}.
CC -!- SIMILARITY: Belongs to the draxin family. {ECO:0000255|HAMAP-
CC Rule:MF_03060}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB301918; BAG80560.1; -; mRNA.
DR EMBL; AK086198; BAC39628.1; -; mRNA.
DR EMBL; AL606929; CAM14900.1; -; Genomic_DNA.
DR EMBL; CU207376; CAQ51921.1; -; Genomic_DNA.
DR EMBL; BC060239; AAH60239.2; -; mRNA.
DR CCDS; CCDS18931.1; -.
DR RefSeq; NP_081702.2; NM_027426.3.
DR AlphaFoldDB; Q6PAL1; -.
DR SMR; Q6PAL1; -.
DR BioGRID; 214047; 1.
DR STRING; 10090.ENSMUSP00000030862; -.
DR GlyGen; Q6PAL1; 1 site.
DR PhosphoSitePlus; Q6PAL1; -.
DR PaxDb; Q6PAL1; -.
DR PRIDE; Q6PAL1; -.
DR ProteomicsDB; 279573; -.
DR Antibodypedia; 2659; 144 antibodies from 25 providers.
DR DNASU; 70433; -.
DR Ensembl; ENSMUST00000030862; ENSMUSP00000030862; ENSMUSG00000029005.
DR GeneID; 70433; -.
DR KEGG; mmu:70433; -.
DR UCSC; uc008vua.2; mouse.
DR CTD; 374946; -.
DR MGI; MGI:1917683; Draxin.
DR VEuPathDB; HostDB:ENSMUSG00000029005; -.
DR eggNOG; ENOG502QUE0; Eukaryota.
DR GeneTree; ENSGT00390000013828; -.
DR HOGENOM; CLU_063473_0_0_1; -.
DR InParanoid; Q6PAL1; -.
DR OMA; NGEQGSF; -.
DR OrthoDB; 860902at2759; -.
DR PhylomeDB; Q6PAL1; -.
DR TreeFam; TF333255; -.
DR BioGRID-ORCS; 70433; 5 hits in 75 CRISPR screens.
DR ChiTaRS; Draxin; mouse.
DR PRO; PR:Q6PAL1; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q6PAL1; protein.
DR Bgee; ENSMUSG00000029005; Expressed in basal plate medulla oblongata and 72 other tissues.
DR Genevisible; Q6PAL1; MM.
DR GO; GO:0005576; C:extracellular region; IDA:MGI.
DR GO; GO:0021960; P:anterior commissure morphogenesis; IMP:UniProtKB.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0021528; P:commissural neuron differentiation in spinal cord; IMP:UniProtKB.
DR GO; GO:0021516; P:dorsal spinal cord development; IMP:UniProtKB.
DR GO; GO:0030900; P:forebrain development; IMP:UniProtKB.
DR GO; GO:0110088; P:hippocampal neuron apoptotic process; IMP:MGI.
DR GO; GO:0030517; P:negative regulation of axon extension; IDA:MGI.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IDA:UniProtKB.
DR GO; GO:0110091; P:negative regulation of hippocampal neuron apoptotic process; IMP:MGI.
DR GO; GO:0010977; P:negative regulation of neuron projection development; IMP:UniProtKB.
DR GO; GO:0051402; P:neuron apoptotic process; IMP:MGI.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR HAMAP; MF_03060; Draxin; 1.
DR InterPro; IPR029094; Draxin.
DR PANTHER; PTHR28610; PTHR28610; 1.
DR Pfam; PF15550; Draxin; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Reference proteome; Secreted; Signal;
KW Wnt signaling pathway.
FT SIGNAL 1..25
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03060"
FT CHAIN 26..343
FT /note="Draxin"
FT /id="PRO_0000273244"
FT REGION 52..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03060"
FT CONFLICT 82
FT /note="T -> S (in Ref. 2; BAG80560 and 4; CAQ51921)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="A -> G (in Ref. 2; BAG80560 and 4; CAQ51921)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="T -> N (in Ref. 3; BAC39628)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="T -> A (in Ref. 2; BAG80560 and 4; CAQ51921)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 38363 MW; 50C71A3654664B93 CRC64;
MAGCPVLRVP TLFLILLLFP ELHTAGTLAS GSSARNLPET HSHLPSSALW VSQASHHGRR
GLGKKDRGPG RPSRAQEGAV VTATKQASQM TLGQPPAGLL QNKELLLGLT LPYPEKEARS
PAWERVKKRG REHKRRRDRL RLHRGRAAIR GPSSLMKKVE PSEDRMLEGT MEESSTSLAP
TMFFLTMTDG ATPTTEESRI LPVTSLRPQT QPRSDGEVMP TLDMALFDWT DYEDLKPEVW
PSAKKKEKHW SHFTSDGNET SPAEGDPCDH HQDCLPGTCC DLREHLCTPH NRGLNNKCFD
DCMCMEGLRC YAKFHRNRRV TRRKGRCVEP ETANGDQGSF INI
