DRB5_ARATH
ID DRB5_ARATH Reviewed; 393 AA.
AC Q8GY79; Q9FLM2;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Double-stranded RNA-binding protein 5;
DE AltName: Full=dsRNA-binding protein 5;
DE Short=AtDRB5;
GN Name=DRB5; OrderedLocusNames=At5g41070; ORFNames=MEE6.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH DCL1; DCL3; DRB1; DRB4 AND DRB5.
RX PubMed=15821876; DOI=10.1007/s11103-004-6853-5;
RA Hiraguri A., Itoh R., Kondo N., Nomura Y., Aizawa D., Murai Y., Koiwa H.,
RA Seki M., Shinozaki K., Fukuhara T.;
RT "Specific interactions between Dicer-like proteins and HYL1/DRB-family
RT dsRNA-binding proteins in Arabidopsis thaliana.";
RL Plant Mol. Biol. 57:173-188(2005).
RN [6]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18625233; DOI=10.1016/j.febslet.2008.07.004;
RA Curtin S.J., Watson J.M., Smith N.A., Eamens A.L., Blanchard C.L.,
RA Waterhouse P.M.;
RT "The roles of plant dsRNA-binding proteins in RNAi-like pathways.";
RL FEBS Lett. 582:2753-2760(2008).
CC -!- FUNCTION: Binds double-stranded RNA. May be involved in RNA-mediated
CC silencing. {ECO:0000269|PubMed:15821876}.
CC -!- SUBUNIT: Heterodimer with DRB1, DRB2 or DRB4. Interacts with DCL1 and
CC DCL3. {ECO:0000269|PubMed:15821876}.
CC -!- INTERACTION:
CC Q8GY79; Q17TI5: BRX; NbExp=3; IntAct=EBI-632672, EBI-4426649;
CC Q8GY79; O04492: DRB1; NbExp=7; IntAct=EBI-632672, EBI-632620;
CC -!- TISSUE SPECIFICITY: Expressed in the shoot apical meristem (SAM).
CC {ECO:0000269|PubMed:18625233}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:18625233}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09709.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010072; BAB09709.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94633.1; -; Genomic_DNA.
DR EMBL; AK117808; BAC42450.1; -; mRNA.
DR EMBL; BT009653; AAP75803.1; -; mRNA.
DR RefSeq; NP_198923.2; NM_123472.3.
DR AlphaFoldDB; Q8GY79; -.
DR SMR; Q8GY79; -.
DR BioGRID; 19360; 10.
DR IntAct; Q8GY79; 9.
DR STRING; 3702.AT5G41070.1; -.
DR PaxDb; Q8GY79; -.
DR PRIDE; Q8GY79; -.
DR ProteomicsDB; 242285; -.
DR EnsemblPlants; AT5G41070.1; AT5G41070.1; AT5G41070.
DR GeneID; 834109; -.
DR Gramene; AT5G41070.1; AT5G41070.1; AT5G41070.
DR KEGG; ath:AT5G41070; -.
DR Araport; AT5G41070; -.
DR TAIR; locus:2163016; AT5G41070.
DR eggNOG; ENOG502QTBA; Eukaryota.
DR HOGENOM; CLU_038996_0_0_1; -.
DR InParanoid; Q8GY79; -.
DR OMA; RSGSCHF; -.
DR OrthoDB; 1031431at2759; -.
DR PhylomeDB; Q8GY79; -.
DR PRO; PR:Q8GY79; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8GY79; baseline and differential.
DR Genevisible; Q8GY79; AT.
DR GO; GO:0003725; F:double-stranded RNA binding; IDA:TAIR.
DR CDD; cd19907; DSRM_AtDRB-like_rpt1; 1.
DR CDD; cd19908; DSRM_AtDRB-like_rpt2; 1.
DR InterPro; IPR044450; AtDRB-like_DSRM_1.
DR InterPro; IPR044451; AtDRB-like_DSRM_2.
DR InterPro; IPR014720; dsRBD_dom.
DR Pfam; PF00035; dsrm; 2.
DR SMART; SM00358; DSRM; 2.
DR PROSITE; PS50137; DS_RBD; 2.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..393
FT /note="Double-stranded RNA-binding protein 5"
FT /id="PRO_0000404656"
FT DOMAIN 1..70
FT /note="DRBM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT DOMAIN 87..155
FT /note="DRBM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT REGION 220..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 335..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..238
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 393 AA; 43635 MW; 027AAD0C05CEE948 CRC64;
MYKNQLQELA QRSCFNLPSY TCIREGPDHA PRFKASVNFN GEIFESPTYC STLRQAEHAA
AEVSLNVLSS RVPSKSLTAK ILDETGIYKN LLQETAHRAG LDLPMYTSVR SGSCHFPGFS
CTVELAGMTF TGESAKTKKQ AEKNAAIAAW SSLKKMSSLD SQDEEKEQEA VARVLSRFKP
KEVRRRETTN QWRRRTSQQD SNKDLLIERL RWINLLTNQA SSSSSTSTPN QHKNSSFISL
IPPPPPPKSS KILPFIQQYK DRSSQEAKTE TATEMINSKA KVNETSTRLS KQMPFSDMNR
YNFVGGCSVN PYSLAPAVQM RSVIPVFAAP PPKPNPNLNP SSLSSSVNEF TSSNNSCSVL
NTPGLGGQEK KNLTREMIKL GSESRILDQT HDS
