ID   DRC3_CHLRE              Reviewed;         524 AA.
AC   A8IVX2;
DT   20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Dynein regulatory complex subunit 3;
DE   AltName: Full=Flagellar-associated protein 134;
GN   Name=DRC3; Synonyms=FAP134; ORFNames=CHLREDRAFT_172483;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=17932292; DOI=10.1126/science.1143609;
RA   Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA   Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA   Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA   Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA   Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA   Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA   Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA   Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA   Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA   Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA   Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA   Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA   Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA   Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA   Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA   Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA   Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA   Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA   Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA   Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA   Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT   "The Chlamydomonas genome reveals the evolution of key animal and plant
RT   functions.";
RL   Science 318:245-250(2007).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   IDENTIFICATION IN THE N-DRC COMPLEX, AND DISRUPTION PHENOTYPE.
RX   PubMed=26063732; DOI=10.1091/mbc.e15-01-0018;
RA   Awata J., Song K., Lin J., King S.M., Sanderson M.J., Nicastro D.,
RA   Witman G.B.;
RT   "DRC3 connects the N-DRC to dynein g to regulate flagellar waveform.";
RL   Mol. Biol. Cell 26:2788-2800(2015).
RN   [3]
RP   FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INTERACTION WITH DRC5, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=23427265; DOI=10.1091/mbc.e12-11-0801;
RA   Bower R., Tritschler D., Vanderwaal K., Perrone C.A., Mueller J., Fox L.,
RA   Sale W.S., Porter M.E.;
RT   "The N-DRC forms a conserved biochemical complex that maintains outer
RT   doublet alignment and limits microtubule sliding in motile axonemes.";
RL   Mol. Biol. Cell 24:1134-1152(2013).
RN   [4]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=25411337; DOI=10.1091/mbc.e14-09-1367;
RA   Oda T., Yanagisawa H., Kikkawa M.;
RT   "Detailed structural and biochemical characterization of the nexin-dynein
RT   regulatory complex.";
RL   Mol. Biol. Cell 26:294-304(2015).
CC   -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a
CC       key regulator of ciliary/flagellar motility which maintains the
CC       alignment and integrity of the distal axoneme and regulates microtubule
CC       sliding in motile axonemes (PubMed:23427265, PubMed:25411337). Required
CC       for normal flagellar waveform (PubMed:26063732).
CC       {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337,
CC       ECO:0000269|PubMed:26063732}.
CC   -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC),
CC       composed of at least DRC1, DRC2, DRC3, DRC4, DRC5, DRC6, DRC7, DRC8,
CC       DRC9, DRC10 and DRC11 (PubMed:25411337, PubMed:23427265). Found in a
CC       complex with DRC3, DRC4, DRC7 and DRC11 (PubMed:26063732).
CC       {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337,
CC       ECO:0000269|PubMed:26063732}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC       {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC   -!- DISRUPTION PHENOTYPE: Defects in DRC3 give the dc3 phenotype
CC       characterized by reduced swimming speed, increased flagellar beat
CC       frequency and abnormal flagellar waveform.
CC   -!- SIMILARITY: Belongs to the DRC3 family. {ECO:0000305}.
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DR   EMBL; DS496125; EDP03601.1; -; Genomic_DNA.
DR   RefSeq; XP_001693032.1; XM_001692980.1.
DR   AlphaFoldDB; A8IVX2; -.
DR   SMR; A8IVX2; -.
DR   STRING; 3055.EDP03601; -.
DR   PaxDb; A8IVX2; -.
DR   PRIDE; A8IVX2; -.
DR   EnsemblPlants; PNW75559; PNW75559; CHLRE_12g531500v5.
DR   GeneID; 5718572; -.
DR   Gramene; PNW75559; PNW75559; CHLRE_12g531500v5.
DR   KEGG; cre:CHLRE_12g531500v5; -.
DR   eggNOG; KOG0531; Eukaryota.
DR   HOGENOM; CLU_026827_0_0_1; -.
DR   InParanoid; A8IVX2; -.
DR   OMA; NSFMEMM; -.
DR   OrthoDB; 568015at2759; -.
DR   GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR   GO; GO:0060285; P:cilium-dependent cell motility; IMP:GeneDB.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   SMART; SM00369; LRR_TYP; 3.
DR   PROSITE; PS51450; LRR; 5.
PE   1: Evidence at protein level;
KW   Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW   Leucine-rich repeat; Repeat.
FT   CHAIN           1..524
FT                   /note="Dynein regulatory complex subunit 3"
FT                   /id="PRO_0000435304"
FT   REPEAT          66..90
FT                   /note="LRR 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          92..110
FT                   /note="LRR 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          111..132
FT                   /note="LRR 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          133..155
FT                   /note="LRR 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          157..183
FT                   /note="LRR 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          250..275
FT                   /note="LRR 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          419..442
FT                   /note="LRR 7"
FT                   /evidence="ECO:0000255"
FT   COILED          198..247
FT                   /evidence="ECO:0000255"
FT   COILED          313..372
FT                   /evidence="ECO:0000255"
FT   COILED          450..514
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   524 AA;  60409 MW;  370669A342D6D463 CRC64;
     MPSLERLIAE VEPNVITESL TRECIQIQGG EPDTAANKKR TMPFRDVECL AFSFKNLACV
     DNLRGLDTLT KLQLDNNQIT KIENLAHLTN LTWLDLSFNK ITAISGLETL TKLVDLSLFN
     NQIAKIENLD TLVNLNVLSL GNNQLSQLDN VMYLRQFKQL RLVNLAGNPI CKSHDYRSYV
     LSHIKDLIYL DYRRVNPADV QAAREQHQDE MIELQEREEQ QSQEEKLNAE RESHEKLMKQ
     ANLEGVETLI DDMVKEDLEW PRLSQVPSLL DPWNEIRDKF NTYTDEFKVA ILEQHNKKKA
     EYEEWLGVVR SYLDEKDAEA RKLIVEYEKA KKRTARVVVD QPLMAESQID NLKVKLMALK
     DQLMAIEMEA VEVLDGLVQE FDRAYSELAE INKGQYNGYF TQVRDLQNSF FNQLTSVAMT
     VFEKYNQENS DIESLPEEAR TLLQDKDSLM NALQASHDAH MGKIDSLEDR LVSNELRSAN
     DLTSSNATWA TKRNRDRISE IINYLERNVL ELEELAGEEE GGEM