DRC3_MOUSE
ID DRC3_MOUSE Reviewed; 523 AA.
AC Q9D5E4;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Dynein regulatory complex subunit 3;
DE AltName: Full=Leucine-rich repeat-containing protein 48;
GN Name=Drc3; Synonyms=Lrrc48;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH TCTE1.
RX PubMed=28630322; DOI=10.1073/pnas.1621279114;
RA Castaneda J.M., Hua R., Miyata H., Oji A., Guo Y., Cheng Y., Zhou T.,
RA Guo X., Cui Y., Shen B., Wang Z., Hu Z., Zhou Z., Sha J.,
RA Prunskaite-Hyyrylainen R., Yu Z., Ramirez-Solis R., Ikawa M., Matzuk M.M.,
RA Liu M.;
RT "TCTE1 is a conserved component of the dynein regulatory complex and is
RT required for motility and metabolism in mouse spermatozoa.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E5370-E5378(2017).
RN [6]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH DRC7.
RX PubMed=31961863; DOI=10.1371/journal.pgen.1008585;
RA Morohoshi A., Miyata H., Shimada K., Nozawa K., Matsumura T., Yanase R.,
RA Shiba K., Inaba K., Ikawa M.;
RT "Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required
RT for sperm flagellum formation and male fertility in mice.";
RL PLoS Genet. 16:e1008585-e1008585(2020).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes. {ECO:0000250|UniProtKB:A8IVX2}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC).
CC Interacts with TCTE1/DRC5 (PubMed:28630322). Interacts with DRC7
CC (PubMed:31961863). {ECO:0000269|PubMed:28630322,
CC ECO:0000269|PubMed:31961863}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q9H069}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q9H069}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000250|UniProtKB:A8IVX2}. Cell projection, cilium,
CC flagellum {ECO:0000269|PubMed:31961863}.
CC -!- SIMILARITY: Belongs to the DRC3 family. {ECO:0000305}.
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DR EMBL; AK015430; BAB29842.1; -; mRNA.
DR EMBL; AL596090; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC018412; AAH18412.1; -; mRNA.
DR CCDS; CCDS36172.1; -.
DR RefSeq; NP_083320.1; NM_029044.2.
DR AlphaFoldDB; Q9D5E4; -.
DR SMR; Q9D5E4; -.
DR BioGRID; 216923; 1.
DR STRING; 10090.ENSMUSP00000104363; -.
DR PhosphoSitePlus; Q9D5E4; -.
DR MaxQB; Q9D5E4; -.
DR PaxDb; Q9D5E4; -.
DR PRIDE; Q9D5E4; -.
DR ProteomicsDB; 279574; -.
DR Antibodypedia; 25546; 30 antibodies from 10 providers.
DR DNASU; 74665; -.
DR Ensembl; ENSMUST00000108723; ENSMUSP00000104363; ENSMUSG00000056598.
DR GeneID; 74665; -.
DR KEGG; mmu:74665; -.
DR UCSC; uc007jfu.1; mouse.
DR CTD; 83450; -.
DR MGI; MGI:1921915; Drc3.
DR VEuPathDB; HostDB:ENSMUSG00000056598; -.
DR eggNOG; KOG0531; Eukaryota.
DR GeneTree; ENSGT00940000159298; -.
DR InParanoid; Q9D5E4; -.
DR OMA; NSFMEMM; -.
DR OrthoDB; 568015at2759; -.
DR PhylomeDB; Q9D5E4; -.
DR TreeFam; TF323974; -.
DR BioGRID-ORCS; 74665; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Drc3; mouse.
DR PRO; PR:Q9D5E4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D5E4; protein.
DR Bgee; ENSMUSG00000056598; Expressed in spermatocyte and 140 other tissues.
DR ExpressionAtlas; Q9D5E4; baseline and differential.
DR Genevisible; Q9D5E4; MM.
DR GO; GO:0005930; C:axoneme; ISO:MGI.
DR GO; GO:0005929; C:cilium; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR SMART; SM00369; LRR_TYP; 3.
DR PROSITE; PS51450; LRR; 5.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..523
FT /note="Dynein regulatory complex subunit 3"
FT /id="PRO_0000227776"
FT REPEAT 44..65
FT /note="LRR 1"
FT REPEAT 66..87
FT /note="LRR 2"
FT REPEAT 88..109
FT /note="LRR 3"
FT REPEAT 110..131
FT /note="LRR 4"
FT REPEAT 132..153
FT /note="LRR 5"
FT DOMAIN 166..204
FT /note="LRRCT"
FT COILED 204..242
FT /evidence="ECO:0000255"
FT COILED 333..393
FT /evidence="ECO:0000255"
SQ SEQUENCE 523 AA; 60827 MW; 1705D0EF302D30A8 CRC64;
MSRPYDSMEP KVMDDDMLKA AVGEQGPQEE AGQLAKQEGI LFKDVLSLQL DFQNILRIDN
LWQFENLKKL QLNNNIIERI EGLTNLIHLV WLDLSFNNIE AIEGLDTLVN LEDLSLSNNR
ISKVDSLDAL VKLQVLSLGN NQISNMMNII YLRRFPCLRT LSLAGNPVSE AEEYKMFIYA
YLSDLVYLDF RRVDEQMREM AKMKHQYSID ELKHREAQLQ MKLEEEQAKQ EKLEEHKMAF
VEHLNGPFLF DSMYSEDVEG NKLSYLPGVR ELLEAYKDKF VIICLNIFEY GLNQQEKRKV
ELDTFNECIQ EAILENQDQG KLKVAKFEEK HLLNLNAIRE ETDLSNIEKK LTECTESIGE
LFNTLMILEM QLVEQLEETI NIFERNITDL VGLFIENVQS LIAQCRDLEN HHHEKLLEIA
INTLEKILKG EMDEDLPDDV RALFVDKDTI VNAVGASHDI HLLKIDNRED ELVTGINSWC
AHLVDKIHKD EIMRNRKRVK EINQFVDHMQ SELDNLECGD IID
