DRC4_DROME
ID DRC4_DROME Reviewed; 479 AA.
AC Q8MT08; Q8T9R5; Q9W4R6;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Dynein regulatory complex subunit 4;
DE AltName: Full=Growth arrest-specific protein 8 {ECO:0000303|PubMed:11751847};
GN Name=Gas8 {ECO:0000303|PubMed:11751847}; ORFNames=CG14271;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11751847; DOI=10.1074/jbc.m106941200;
RA Yeh S.-D., Chen Y.-J., Chang A.C.Y., Ray R., She B.-R., Lee W.-S.,
RA Chiang H.-S., Cohen S.N., Lin-Chao S.;
RT "Isolation and properties of Gas8, a growth arrest-specific gene regulated
RT during male gametogenesis to produce a protein associated with the sperm
RT motility apparatus.";
RL J. Biol. Chem. 277:6311-6317(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Cytoskeletal linker which probably functions in axonemal and
CC non-axonemal dynein regulation. May play a role in the spermatozoa
CC motility (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm,
CC cytoskeleton, flagellum basal body {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DRC4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL79828.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF468957; AAL79828.1; ALT_INIT; mRNA.
DR EMBL; AE014298; AAF45877.3; -; Genomic_DNA.
DR EMBL; AY118453; AAM49822.1; -; mRNA.
DR RefSeq; NP_001284840.1; NM_001297911.1.
DR RefSeq; NP_001284841.1; NM_001297912.1.
DR RefSeq; NP_570065.2; NM_130709.2.
DR AlphaFoldDB; Q8MT08; -.
DR SMR; Q8MT08; -.
DR BioGRID; 57846; 2.
DR DIP; DIP-20314N; -.
DR IntAct; Q8MT08; 5.
DR STRING; 7227.FBpp0070564; -.
DR PaxDb; Q8MT08; -.
DR DNASU; 31321; -.
DR EnsemblMetazoa; FBtr0070589; FBpp0070564; FBgn0029667.
DR EnsemblMetazoa; FBtr0344969; FBpp0311223; FBgn0029667.
DR GeneID; 31321; -.
DR KEGG; dme:Dmel_CG14271; -.
DR CTD; 2622; -.
DR FlyBase; FBgn0029667; Gas8.
DR VEuPathDB; VectorBase:FBgn0029667; -.
DR eggNOG; ENOG502QQDA; Eukaryota.
DR HOGENOM; CLU_045343_0_0_1; -.
DR InParanoid; Q8MT08; -.
DR OMA; SWEHEVL; -.
DR OrthoDB; 1257437at2759; -.
DR PhylomeDB; Q8MT08; -.
DR BioGRID-ORCS; 31321; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31321; -.
DR PRO; PR:Q8MT08; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029667; Expressed in testis and 9 other tissues.
DR ExpressionAtlas; Q8MT08; baseline and differential.
DR Genevisible; Q8MT08; DM.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR InterPro; IPR039308; GAS8.
DR InterPro; IPR025593; GAS8_dom.
DR PANTHER; PTHR31543; PTHR31543; 1.
DR Pfam; PF13851; GAS; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Microtubule; Reference proteome.
FT CHAIN 1..479
FT /note="Dynein regulatory complex subunit 4"
FT /id="PRO_0000220380"
FT COILED 28..93
FT /evidence="ECO:0000255"
FT COILED 117..170
FT /evidence="ECO:0000255"
FT COILED 210..347
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 57168 MW; 4C4EC1E487A0AFEE CRC64;
MPPKGKKGKK GKKLPVLIDG VDTSAMTRDQ LEAFALRLKA EMDREREERN YFQLERDKIR
TFWEITRQQL DETRYELQQK DKEIEATQDL ADIDTKHVMQ QMKHLQFENH NRLGEVRAEA
MTQLKLAQEH HVLQENELQR DKRQLRRMLR ERMEMSEMQL RQMEAHFNEK LLEQRITFER
ERKDNEMLHE EKMIEQKAKL DLFYGTQMFE VEERKNQQIK DLQDHHDLAF NDMKNYYNDI
TLNNLALIGS MKEQLEHLRK QAERSDRIAA DTAAENRRLK EPLEHANIQL NEYRRKLEFY
ERDKQQLSRL KTRNTRLEKK VKGLTWEAET LILRNDSLVA EREGLKERFN DVIVELQQKT
GLKNVLLERK IAALMREDEK RSIVLHETIA TCAPNFAEKL TSLDERVGNI IDEKNKIILD
LRYEVTKARK AHDDLLETYE CKLKQYGVPT DELGFKPIRN RDQQQLYVCG PAGIITENK
