DRC5_CHLRE
ID DRC5_CHLRE Reviewed; 390 AA.
AC A8HMZ4;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Dynein regulatory complex subunit 5 {ECO:0000303|PubMed:23427265, ECO:0000303|PubMed:25411337};
DE AltName: Full=Flagellar-associated protein 155;
GN Name=DRC5 {ECO:0000303|PubMed:23427265, ECO:0000303|PubMed:25411337};
GN Synonyms=FAP155; ORFNames=CHLREDRAFT_190077;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION
RP WITH DRC1; DRC2; DRC3; DRC4; DRC7 AND DRC11, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=23427265; DOI=10.1091/mbc.e12-11-0801;
RA Bower R., Tritschler D., Vanderwaal K., Perrone C.A., Mueller J., Fox L.,
RA Sale W.S., Porter M.E.;
RT "The N-DRC forms a conserved biochemical complex that maintains outer
RT doublet alignment and limits microtubule sliding in motile axonemes.";
RL Mol. Biol. Cell 24:1134-1152(2013).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=25411337; DOI=10.1091/mbc.e14-09-1367;
RA Oda T., Yanagisawa H., Kikkawa M.;
RT "Detailed structural and biochemical characterization of the nexin-dynein
RT regulatory complex.";
RL Mol. Biol. Cell 26:294-304(2015).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes (PubMed:23427265, PubMed:25411337). May play
CC a role in the assembly of N-DRC (PubMed:23427265).
CC {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC),
CC composed of at least DRC1, DRC2, DRC3, DRC4, DRC5, DRC6, DRC7, DRC8,
CC DRC9, DRC10 and DRC11 (PubMed:23427265, PubMed:25411337). Interacts
CC with DRC1, DRC2, DRC3, DRC4, DRC7 and DRC11 (PubMed:25411337).
CC {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:A6H639}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC Note=Detected along the length of the sperm flagellum.
CC {ECO:0000250|UniProtKB:A6H639}.
CC -!- DISRUPTION PHENOTYPE: Defects in DRC5 give the sup-pf4 phenotype
CC characterized by disruption of the assembly of several other N-DRC
CC subunits. {ECO:0000269|PubMed:23427265}.
CC -!- SIMILARITY: Belongs to the DRC5 family. {ECO:0000305}.
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DR EMBL; DS496108; EDP09462.1; -; Genomic_DNA.
DR RefSeq; XP_001689724.1; XM_001689672.1.
DR AlphaFoldDB; A8HMZ4; -.
DR SMR; A8HMZ4; -.
DR STRING; 3055.EDP09462; -.
DR PaxDb; A8HMZ4; -.
DR GeneID; 5715659; -.
DR KEGG; cre:CHLRE_01g045350v5; -.
DR eggNOG; KOG0619; Eukaryota.
DR eggNOG; KOG4308; Eukaryota.
DR HOGENOM; CLU_029623_1_2_1; -.
DR InParanoid; A8HMZ4; -.
DR OrthoDB; 519026at2759; -.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13516; LRR_6; 4.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Leucine-rich repeat; Repeat.
FT CHAIN 1..390
FT /note="Dynein regulatory complex subunit 5"
FT /id="PRO_0000444015"
FT REPEAT 182..205
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 210..233
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 238..261
FT /note="LRR 3"
FT /evidence="ECO:0000255"
SQ SEQUENCE 390 AA; 43503 MW; AAC13B6429265BB3 CRC64;
MAAPNLITPL RDICVKVVAA NFEGCPTFGP LPDKYVKRII DILPLDLPLE LVGSLIADED
YWRRRSQARW KNCEVAAHGY SWKQLFFERN LMEFLEQYDP AVTDLSSLKR LLTYSRRFVQ
TVHIRQLPSH LDLQILFECM VNTPSSLALS YNLKEVGMDY DRSLFGMKLS DCRALAKALE
HTETLTHLDL SNNSLDDDKV RMLASGLVEN LSITHLNLSH NKIADRGVRA LAKLLDGHSV
ISLLELHDNQ IHTEGAESLA RAFKSNQCLL SVNLRLNRMG DEGCKAVVES VRGSPTLQRL
NISANAAGPG TAAAVVALLR LNNTLTELDV SCNQFGEDAC GNVRRALEQN GSVRLMDVRM
TGINPDDEMA IAENLRARQE RVDKARVLGK
