DRC5_MOUSE
ID DRC5_MOUSE Reviewed; 498 AA.
AC A6H639; Q62294;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Dynein regulatory complex subunit 5 {ECO:0000303|PubMed:28630322};
DE AltName: Full=T-complex-associated testis-expressed protein 1;
DE Short=Tcte-1;
GN Name=Tcte1; Synonyms=D17sil1, Drc5 {ECO:0000303|PubMed:28630322};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=2568335; DOI=10.1007/bf02421467;
RA Sarvetnick N., Tsai J.-Y., Fox H., Pilder S.H., Silver L.M.;
RT "A mouse chromosome 17 gene encodes a testes-specific transcript with
RT unusual properties.";
RL Immunogenetics 30:34-41(1989).
RN [2]
RP ERRATUM OF PUBMED:2568335.
RA Sarvetnick N., Tsai J.-Y., Fox H., Pilder S.H., Silver L.;
RL Immunogenetics 31:283-284(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND INTERACTION WITH FBXL13; DRC3 AND DRC7.
RX PubMed=28630322; DOI=10.1073/pnas.1621279114;
RA Castaneda J.M., Hua R., Miyata H., Oji A., Guo Y., Cheng Y., Zhou T.,
RA Guo X., Cui Y., Shen B., Wang Z., Hu Z., Zhou Z., Sha J.,
RA Prunskaite-Hyyrylainen R., Yu Z., Ramirez-Solis R., Ikawa M., Matzuk M.M.,
RA Liu M.;
RT "TCTE1 is a conserved component of the dynein regulatory complex and is
RT required for motility and metabolism in mouse spermatozoa.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E5370-E5378(2017).
RN [6]
RP INTERACTION WITH DRC7.
RX PubMed=31961863; DOI=10.1371/journal.pgen.1008585;
RA Morohoshi A., Miyata H., Shimada K., Nozawa K., Matsumura T., Yanase R.,
RA Shiba K., Inaba K., Ikawa M.;
RT "Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required
RT for sperm flagellum formation and male fertility in mice.";
RL PLoS Genet. 16:e1008585-e1008585(2020).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes. May play a role in the assembly of N-DRC
CC (By similarity). Required for sperm motility (PubMed:28630322).
CC {ECO:0000250|UniProtKB:A8HMZ4, ECO:0000269|PubMed:28630322}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC)
CC Interacts with FBXL13/DRC6, DRC3 and DRC7 (PubMed:28630322,
CC PubMed:31961863). {ECO:0000269|PubMed:28630322,
CC ECO:0000269|PubMed:31961863}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|Ref.2}. Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000250|UniProtKB:A8HMZ4}. Note=Detected along the length of the
CC sperm flagellum. {ECO:0000269|Ref.2}.
CC -!- TISSUE SPECIFICITY: Testis-specific (at protein level).
CC {ECO:0000269|PubMed:2568335, ECO:0000269|Ref.2}.
CC -!- DISRUPTION PHENOTYPE: Male mice are sterile due to asthenozoospermia
CC (sperm with diminished progressive motility) and the diminished
CC motility is due to aberrant flagellar function, with sperm tails
CC contracting toward one side and generating a circular path for sperm.
CC {ECO:0000269|PubMed:28630322}.
CC -!- SIMILARITY: Belongs to the DRC5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA40406.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M28821; AAA40406.1; ALT_FRAME; mRNA.
DR EMBL; BC145741; AAI45742.1; -; mRNA.
DR CCDS; CCDS37629.1; -.
DR PIR; A45841; A45841.
DR PIR; A45878; A45878.
DR RefSeq; NP_038716.2; NM_013688.2.
DR AlphaFoldDB; A6H639; -.
DR SMR; A6H639; -.
DR STRING; 10090.ENSMUSP00000109175; -.
DR iPTMnet; A6H639; -.
DR PhosphoSitePlus; A6H639; -.
DR MaxQB; A6H639; -.
DR PaxDb; A6H639; -.
DR PRIDE; A6H639; -.
DR ProteomicsDB; 263098; -.
DR Antibodypedia; 30669; 80 antibodies from 19 providers.
DR Ensembl; ENSMUST00000113547; ENSMUSP00000109175; ENSMUSG00000023949.
DR Ensembl; ENSMUST00000233588; ENSMUSP00000156920; ENSMUSG00000023949.
DR GeneID; 21645; -.
DR KEGG; mmu:21645; -.
DR UCSC; uc008cqt.1; mouse.
DR CTD; 202500; -.
DR MGI; MGI:98640; Tcte1.
DR VEuPathDB; HostDB:ENSMUSG00000023949; -.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000159341; -.
DR HOGENOM; CLU_029623_1_0_1; -.
DR InParanoid; A6H639; -.
DR OMA; WINIYME; -.
DR OrthoDB; 519026at2759; -.
DR PhylomeDB; A6H639; -.
DR TreeFam; TF325870; -.
DR BioGRID-ORCS; 21645; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Tcte1; mouse.
DR PRO; PR:A6H639; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; A6H639; protein.
DR Bgee; ENSMUSG00000023949; Expressed in spermatid and 76 other tissues.
DR Genevisible; A6H639; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13516; LRR_6; 4.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..498
FT /note="Dynein regulatory complex subunit 5"
FT /id="PRO_0000326527"
FT REPEAT 276..299
FT /note="LRR 1"
FT REPEAT 306..327
FT /note="LRR 2"
FT REPEAT 333..353
FT /note="LRR 3"
FT REPEAT 361..382
FT /note="LRR 4"
FT REPEAT 389..409
FT /note="LRR 5"
FT REPEAT 417..438
FT /note="LRR 6"
FT REGION 27..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 30
FT /note="S -> N (in Ref. 1; AAA40406)"
FT /evidence="ECO:0000305"
FT CONFLICT 498
FT /note="V -> A (in Ref. 1; AAA40406)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 498 AA; 55518 MW; 91D12BF57E46C874 CRC64;
MQETPSVPSN SSSHSQSVLT IQRQVSALGS SSTGPTSLKT SSTPTPGQLK TKVPNVRRMR
RIISEDAEWS LAIVPLLTEL CIQHIVKNFQ NNPILKQLPL EHQKKVLSNL PPELPLTVTA
NLIDDENYWH RCCIKRWSVC HVSRHGGSWK RMFFERHLEN LLKLFIPGTT DPNVILDLLP
LCRNYVRRIH VDQFLPPVRM PTPLQGEEQS DSGSEGEGSE PEKDHYQLQT LVGGLKHLEE
LDLVYGVKDC GMNFEWNLFL FTYRDCYSLA ATIKACHTLK IFKLTRSKVD DDKARILIRS
LLDHPALEEL DLSHNLIGDR GARAAAKLLS HSRLRVLNLA NNQLQAPGAQ SLAHALAHNT
NLVFLNLRLN CIEDEGGQAI AHALETNKCL SVLHLGGNKL SEPTATLLSQ MLTVNTTLVS
LNLSCNHIGQ DGGKQLLEGI SDNKTILEFD LRLSDVSQES EYLIGQVLHA NREAARQRTL
NPGHFSSPTN NCTENSVV
