DRC7_DROME
ID DRC7_DROME Reviewed; 897 AA.
AC Q4V516;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Coiled-coil domain-containing protein lobo;
DE AltName: Full=Lost boys protein;
GN Name=lobo; ORFNames=CG13662;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=17534367; DOI=10.1038/ng2049;
RA Chintapalli V.R., Wang J., Dow J.A.;
RT "Using FlyAtlas to identify better Drosophila melanogaster models of human
RT disease.";
RL Nat. Genet. 39:715-720(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=21289096; DOI=10.1091/mbc.e10-04-0331;
RA Yang Y., Cochran D.A., Gargano M.D., King I., Samhat N.K., Burger B.P.,
RA Sabourin K.R., Hou Y., Awata J., Parry D.A., Marshall W.F., Witman G.B.,
RA Lu X.;
RT "Regulation of flagellar motility by the conserved flagellar protein
RT CG34110/Ccdc135/FAP50.";
RL Mol. Biol. Cell 22:976-987(2011).
CC -!- FUNCTION: Key component of the nexin-dynein regulatory complex (N-DRC),
CC essential for N-DRC integrity. Involved in the regulation of flagellar
CC motility (By similarity). Involved in sperm motility. Required for the
CC sperm to enter in the coiled storage seminal receptacle (SR) tubule
CC (PubMed:21289096). {ECO:0000250|UniProtKB:A8JAM0,
CC ECO:0000269|PubMed:21289096}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:21289096}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:A8JAM0}. Note=Detected as regular puncta along
CC the flagellum. {ECO:0000269|PubMed:21289096}.
CC -!- TISSUE SPECIFICITY: Testis-specific (at protein level).
CC {ECO:0000269|PubMed:17534367, ECO:0000269|PubMed:21289096}.
CC -!- SIMILARITY: Belongs to the DRC7 family. {ECO:0000305}.
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DR EMBL; AE014297; ABI31207.1; -; Genomic_DNA.
DR EMBL; BT022813; AAY55229.1; -; mRNA.
DR EMBL; BT022840; AAY55256.1; -; mRNA.
DR EMBL; BT022876; AAY55292.1; -; mRNA.
DR RefSeq; NP_001036757.1; NM_001043292.2.
DR AlphaFoldDB; Q4V516; -.
DR SMR; Q4V516; -.
DR BioGRID; 607093; 18.
DR STRING; 7227.FBpp0110094; -.
DR PaxDb; Q4V516; -.
DR PRIDE; Q4V516; -.
DR DNASU; 4379864; -.
DR EnsemblMetazoa; FBtr0110797; FBpp0110094; FBgn0083946.
DR GeneID; 4379864; -.
DR KEGG; dme:Dmel_CG34110; -.
DR UCSC; CG34110-RC; d. melanogaster.
DR CTD; 4379864; -.
DR FlyBase; FBgn0083946; lobo.
DR VEuPathDB; VectorBase:FBgn0083946; -.
DR eggNOG; ENOG502QRNZ; Eukaryota.
DR HOGENOM; CLU_016052_0_0_1; -.
DR InParanoid; Q4V516; -.
DR OMA; SETMFRI; -.
DR OrthoDB; 535554at2759; -.
DR PhylomeDB; Q4V516; -.
DR BioGRID-ORCS; 4379864; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 4379864; -.
DR PRO; PR:Q4V516; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0083946; Expressed in testis and 10 other tissues.
DR Genevisible; Q4V516; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IDA:FlyBase.
DR GO; GO:0048870; P:cell motility; IBA:GO_Central.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:FlyBase.
DR GO; GO:0046693; P:sperm storage; IMP:FlyBase.
DR InterPro; IPR033551; DRC7/lobo.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR002931; Transglutaminase-like.
DR PANTHER; PTHR35249; PTHR35249; 1.
DR Pfam; PF01841; Transglut_core; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome.
FT CHAIN 1..897
FT /note="Coiled-coil domain-containing protein lobo"
FT /id="PRO_0000415787"
FT REGION 27..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 269..306
FT /evidence="ECO:0000255"
FT COILED 801..858
FT /evidence="ECO:0000255"
SQ SEQUENCE 897 AA; 105242 MW; D1BDDBF959EA8DCD CRC64;
MPKVLFQKYK KVVSNIDPSR YSAKFKEIDE QRRSQGSESD FADEMEGEFE AEMEEMGSSE
FSLSEGEPSL NIGKIDLSFP ETVEEFENSP QCYPPSYYTL SPKERLLLLY AENFRKQLVL
SYPKRRAMVL ALPNECKVQK FVCTTIRPTA FIYTQLISSV EEIAKFVADF IQYEPLQDPI
NLPNRLISPE TLLRKRKGNS YEMATLLVSM LIGAGHPALV VSGVAREETI LNDQLAVPYP
YPIVEVEVEE VKPKVEKPGQ KYKLRGLPDL KSHLEEDMAE VHRQKEAEEK RIQDEIIRKQ
MEDLELLAVD RYHYRRSHAW VVIINNAPWS VKPKTTYTDV NGDVVEAPPT AIFIEPSTGF
ICETGCKQYI LVDSIWNEYN YYVCKQKHQR VSELRWDLRD TKDWEHMLPG EPPEMRIYKM
ASDENVADEE RDISEEKHLD CIGSWVERLH IGLADFEQRF PSSEKKIQYK GAIHERFSPY
SQRDGKVMQL TIFNNDECTI PKVRYEYYEN RSDLMRHVKY TYATDQFEEI FNKGRNDSLK
SIEYFSDPSQ QRKVHFFSAS RIDSLELMVV EPGSLILHYK DRSDKCWYKE FEFTPAGNVL
KKVTEKFLKA SANDVCSNDI ATRTFLFQQN KIVLKFHYTF GALTATVVEF TKPPRPDYGK
ELIYDEKLTK IYKANPLDPT RTNLELYRLL CEQLQYEDHL RKNFEKIFED INNVFDLRKS
EKAEPKLKFS IFDPLRNGAA RAIRMRQFEE EEELKKEIAS KPADFLAPYL VPYKHQEELT
PEQSQNAYNA CLNDLKSRFV SLLNNLQRHY EDLTSESKSL NRFLNKFENQ FNNYDYKRLV
QQSKDLELNK RMVQQRLTLT HEESQKKYEV VKNSLLKDPR LNFKKDDLAR RASETSK
