DRC7_MOUSE
ID DRC7_MOUSE Reviewed; 876 AA.
AC Q6V3W6; G3X9B0; Q148T2;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 4.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Dynein regulatory complex subunit 7;
DE AltName: Full=Coiled-coil domain-containing protein 135;
DE AltName: Full=Coiled-coil domain-containing protein lobo homolog;
DE AltName: Full=Spermatogenesis-related gene in late stages of spermatogenesis cells protein;
GN Name=Drc7; Synonyms=Ccdc135, Gm770, Srgl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=15156272; DOI=10.1093/abbs/36.5.315;
RA Guo R., Ma P.P., Ma J., Ge Y.H., Xue S.P., Han D.S.;
RT "Cloning and characterization of a novel gene SRG-L expressed in late
RT stages of mouse spermatogenic cells.";
RL Acta Biochim. Biophys. Sin. 36:315-322(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ; TISSUE=Testis;
RX PubMed=16804880; DOI=10.1002/mrd.20485;
RA Ma Q., Wang H., Guo R., Wang H., Ge Y., Ma J., Xue S., Han D.;
RT "Molecular cloning and characterization of SRG-L, a novel mouse gene
RT developmentally expressed in spermatogenic cells.";
RL Mol. Reprod. Dev. 73:1075-1083(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=17971504; DOI=10.1152/physiolgenomics.00128.2007;
RA McClintock T.S., Glasser C.E., Bose S.C., Bergman D.A.;
RT "Tissue expression patterns identify mouse cilia genes.";
RL Physiol. Genomics 32:198-206(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [8]
RP INTERACTION WITH TCTE1.
RX PubMed=28630322; DOI=10.1073/pnas.1621279114;
RA Castaneda J.M., Hua R., Miyata H., Oji A., Guo Y., Cheng Y., Zhou T.,
RA Guo X., Cui Y., Shen B., Wang Z., Hu Z., Zhou Z., Sha J.,
RA Prunskaite-Hyyrylainen R., Yu Z., Ramirez-Solis R., Ikawa M., Matzuk M.M.,
RA Liu M.;
RT "TCTE1 is a conserved component of the dynein regulatory complex and is
RT required for motility and metabolism in mouse spermatozoa.";
RL Proc. Natl. Acad. Sci. U.S.A. 114:E5370-E5378(2017).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND INTERACTION WITH
RP TTCE1; DRC3 AND GAS8.
RX PubMed=31961863; DOI=10.1371/journal.pgen.1008585;
RA Morohoshi A., Miyata H., Shimada K., Nozawa K., Matsumura T., Yanase R.,
RA Shiba K., Inaba K., Ikawa M.;
RT "Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required
RT for sperm flagellum formation and male fertility in mice.";
RL PLoS Genet. 16:e1008585-e1008585(2020).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC) a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes (By similarity). Essential for male
CC fertility, sperm head morphogenesis and sperm flagellum formation
CC (PubMed:31961863). Not required for ciliogenesis in the brain and
CC trachea (PubMed:31961863). {ECO:0000250|UniProtKB:A8JAM0,
CC ECO:0000269|PubMed:31961863}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC) (By
CC similarity). Interacts with TCTE1/DRC5 (PubMed:28630322,
CC PubMed:31961863). Interacts with DRC3 and GAS8/DRC4 (PubMed:31961863).
CC {ECO:0000250|UniProtKB:A8JAM0, ECO:0000269|PubMed:28630322,
CC ECO:0000269|PubMed:31961863}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:A8JAM0}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:A8JAM0}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000250|UniProtKB:A8JAM0}. Note=Associated with the outer
CC doublet microtubules (OD). {ECO:0000250|UniProtKB:A8JAM0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=SRG-L;
CC IsoId=Q6V3W6-1; Sequence=Displayed;
CC Name=2; Synonyms=SRG-S;
CC IsoId=Q6V3W6-2; Sequence=VSP_023440, VSP_023441;
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Expressed in diplotene and pachytene
CC spermytocytes, and in round and elongating spermatids (at protein
CC level). Strongly expressed in spleen and testis, faintly expressed in
CC kidney, ovary and thymus. {ECO:0000269|PubMed:15156272,
CC ECO:0000269|PubMed:16804880}.
CC -!- TISSUE SPECIFICITY: Abundantly expressed in the testis and is weakly
CC expressed in the brain, thymus, lung and ovary (PubMed:31961863).
CC Expressed in ciliated cells (PubMed:17971504).
CC {ECO:0000269|PubMed:17971504, ECO:0000269|PubMed:31961863}.
CC -!- DISRUPTION PHENOTYPE: Male mice were infertile due to their short
CC immotile spermatozoa (PubMed:31961863). Axoneme formation and manchette
CC removal are impaired in spermatids (PubMed:31961863). No morphological
CC abnormalities seen in multicilia of ependymal and tracheal epithelia
CC and cilia motility is not impaired in ependymal cilia
CC (PubMed:31961863). {ECO:0000269|PubMed:31961863}.
CC -!- SIMILARITY: Belongs to the DRC7 family. {ECO:0000305}.
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DR EMBL; AY352586; AAQ55489.2; -; mRNA.
DR EMBL; AC103935; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466525; EDL11157.1; -; Genomic_DNA.
DR EMBL; BC117982; AAI17983.1; -; mRNA.
DR EMBL; BC117983; AAI17984.1; -; mRNA.
DR CCDS; CCDS40444.1; -. [Q6V3W6-1]
DR RefSeq; NP_001036180.2; NM_001042715.3. [Q6V3W6-1]
DR RefSeq; XP_006531195.1; XM_006531132.2. [Q6V3W6-1]
DR AlphaFoldDB; Q6V3W6; -.
DR SMR; Q6V3W6; -.
DR STRING; 10090.ENSMUSP00000053972; -.
DR iPTMnet; Q6V3W6; -.
DR PhosphoSitePlus; Q6V3W6; -.
DR MaxQB; Q6V3W6; -.
DR PaxDb; Q6V3W6; -.
DR PRIDE; Q6V3W6; -.
DR ProteomicsDB; 279575; -. [Q6V3W6-1]
DR ProteomicsDB; 279576; -. [Q6V3W6-2]
DR Antibodypedia; 49050; 82 antibodies from 13 providers.
DR DNASU; 330830; -.
DR Ensembl; ENSMUST00000058479; ENSMUSP00000053972; ENSMUSG00000031786. [Q6V3W6-1]
DR GeneID; 330830; -.
DR KEGG; mmu:330830; -.
DR UCSC; uc009mxr.1; mouse. [Q6V3W6-2]
DR UCSC; uc009mxs.1; mouse. [Q6V3W6-1]
DR CTD; 84229; -.
DR MGI; MGI:2685616; Drc7.
DR VEuPathDB; HostDB:ENSMUSG00000031786; -.
DR eggNOG; ENOG502QRNZ; Eukaryota.
DR GeneTree; ENSGT00390000004913; -.
DR HOGENOM; CLU_016052_0_0_1; -.
DR InParanoid; Q6V3W6; -.
DR OMA; SETMFRI; -.
DR OrthoDB; 535554at2759; -.
DR PhylomeDB; Q6V3W6; -.
DR TreeFam; TF323665; -.
DR BioGRID-ORCS; 330830; 4 hits in 72 CRISPR screens.
DR PRO; PR:Q6V3W6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q6V3W6; protein.
DR Bgee; ENSMUSG00000031786; Expressed in vasculature of organ and 32 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0048870; P:cell motility; IBA:GO_Central.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR033551; DRC7/lobo.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR PANTHER; PTHR35249; PTHR35249; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW Cytoskeleton; Differentiation; Flagellum; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..876
FT /note="Dynein regulatory complex subunit 7"
FT /id="PRO_0000279436"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 385..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1..33
FT /evidence="ECO:0000255"
FT COILED 258..295
FT /evidence="ECO:0000255"
FT COILED 679..710
FT /evidence="ECO:0000255"
FT COILED 784..809
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..407
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 288..295
FT /note="EAEKAKPD -> VGLHDTAL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023440"
FT VAR_SEQ 296..876
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023441"
FT CONFLICT 9
FT /note="Missing (in Ref. 2; AAQ55489)"
FT /evidence="ECO:0000305"
FT CONFLICT 603
FT /note="D -> G (in Ref. 2; AAQ55489)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 876 AA; 103269 MW; DD009EA45F68AEF0 CRC64;
MEVLREKVEE EEEAEREEAA ERAERTEKLE RVTKSAEVSR EGTILSQDEL RDLEGKLMAI
EIPTQADHSV ISQAPVDVTK LPPSYTTNSL KEEHLLLVAD NFSRQYSHLC PDRVPLFLHP
LNECNVPKFV STTLRPTLMP YPELYNWDSC AQFVSDFLTM VPLVDPLKPP THLYSSTTVL
KCQKGNCFDF STLLCSMLIG SGYDAYCVNG YGSLDLCLMD LTREVCPLTV KAKEIVKKKE
KTVPKKYSIK PPRDLTSKFE QEQEEKRIQE IKDLEQRRLK EEEDRILEAE KAKPDPLHGL
RVHSWVLVLA GKREVPESFF IDPLTARSYS TKDEHFLGIE SLWNHKNYWI NMQDCWNCCK
DLIFDLGDPV RWEYMLLGTD KPHLSLTEED EEGLDDDDDD VEDLGKEEED KSFDMPSSWV
SQIEITPEEF ETRCPSGKKV IQYKKAQLEK WSPYLNNNGL VCRLTTYEDQ QCTKVLEIKE
WYQNREDMLE LKHINKTTGL HVDYFKPGHP QALCVHSYKS MLPEMDRVME FYKKIRVDGL
VKREETPMTM TEYYEGRSDF LAYRHVNFGP RVKKLSQSSV ESNPRPMVKI TERFSRNPEK
PADEDVAERL FLIVEERIQL RYHCRDDYIT ASKREFLRRM EVDSKGNKII MTPEMCISYE
VEPMEHTKKL LYQYETMNQL KNEEKLSRHQ AWESELEVLE ILKLREEEEE AHTLTISIYD
TKRNEKSKEY REAMERVLHE EHLRQVEAQL DYLAPFLAQL PPGEKLTRWQ AVRLKDECLS
DFKQRLIDKA NLIQARFEKE TQELQKKQQW YQENQVTLTP EDENLYLSYC SQAMFRIRIL
EQRLNRHKEL APLKYLALEE KLYKDPRLID FVKVFV
