DRC8_CHLRE
ID DRC8_CHLRE Reviewed; 166 AA.
AC A8J3A0;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=Dynein regulatory complex protein 8 {ECO:0000303|PubMed:23427265, ECO:0000303|PubMed:25411337};
DE AltName: Full=Flagellar associated protein 200;
GN Name=DRC8 {ECO:0000303|PubMed:23427265, ECO:0000303|PubMed:25411337};
GN Synonyms=FAP200;
GN ORFNames=CHLRE_16g676645v5 {ECO:0000305}, CHLREDRAFT_174407;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=23427265; DOI=10.1091/mbc.e12-11-0801;
RA Bower R., Tritschler D., Vanderwaal K., Perrone C.A., Mueller J., Fox L.,
RA Sale W.S., Porter M.E.;
RT "The N-DRC forms a conserved biochemical complex that maintains outer
RT doublet alignment and limits microtubule sliding in motile axonemes.";
RL Mol. Biol. Cell 24:1134-1152(2013).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=25411337; DOI=10.1091/mbc.e14-09-1367;
RA Oda T., Yanagisawa H., Kikkawa M.;
RT "Detailed structural and biochemical characterization of the nexin-dynein
RT regulatory complex.";
RL Mol. Biol. Cell 26:294-304(2015).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC), a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes (PubMed:23427265, PubMed:25411337).
CC {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC),
CC composed of DRC1, DRC2, DRC3, DRC4, DRC5, DRC6, DRC7, DRC8, DRC9, DRC10
CC and DRC11 (PubMed:23427265, PubMed:25411337).
CC {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:23427265, ECO:0000269|PubMed:25411337}.
CC -!- SIMILARITY: Belongs to the DRC8 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS496135; EDP01226.1; -; Genomic_DNA.
DR EMBL; CM008977; PNW72406.1; -; Genomic_DNA.
DR RefSeq; XP_001695889.1; XM_001695837.1.
DR AlphaFoldDB; A8J3A0; -.
DR SMR; A8J3A0; -.
DR STRING; 3055.EDP01226; -.
DR PaxDb; A8J3A0; -.
DR PRIDE; A8J3A0; -.
DR EnsemblPlants; PNW72406; PNW72406; CHLRE_16g676645v5.
DR GeneID; 5721352; -.
DR Gramene; PNW72406; PNW72406; CHLRE_16g676645v5.
DR KEGG; cre:CHLRE_16g676645v5; -.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_19_2_1; -.
DR InParanoid; A8J3A0; -.
DR OMA; WYEDYAQ; -.
DR OrthoDB; 1096220at2759; -.
DR Proteomes; UP000006906; Chromosome 16.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome.
FT CHAIN 1..166
FT /note="Dynein regulatory complex protein 8"
FT /id="PRO_0000444018"
FT DOMAIN 95..130
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 166 AA; 18647 MW; 0FBC2B508F1A26DA CRC64;
MSKLNDKHER VRARVREAFI LFEHKEGSRL VDFKDVPTAV RACGVNPTAV QMTHILDQLA
ALNAETDATG YVGLENFELV VCNFLIQQEA SLFRDDYHTL LRAFRAFDPD GRGFIDAESF
KSLLTGKGEA LSEDELAKMM TVAADGEGKI WYEDYAQRLA NDGRTI
