ID   DRC8_MOUSE              Reviewed;         164 AA.
AC   Q9CQ46; Q9CS07;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Dynein regulatory complex protein 8 {ECO:0000250|UniProtKB:A8J3A0};
DE   AltName: Full=EF-hand calcium-binding domain-containing protein 2;
GN   Name=Efcab2; Synonyms=Drc8 {ECO:0000250|UniProtKB:A8J3A0};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Liver;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC), a
CC       key regulator of ciliary/flagellar motility which maintains the
CC       alignment and integrity of the distal axoneme and regulates microtubule
CC       sliding in motile axonemes. {ECO:0000250|UniProtKB:A8J3A0}.
CC   -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC).
CC       {ECO:0000250|UniProtKB:A8J3A0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC       {ECO:0000250|UniProtKB:A8J3A0}.
CC   -!- SIMILARITY: Belongs to the DRC8 family. {ECO:0000305}.
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DR   EMBL; AK010880; BAB27243.1; -; mRNA.
DR   EMBL; AK015157; BAB29727.1; -; mRNA.
DR   EMBL; AK018894; BAB31477.1; -; mRNA.
DR   EMBL; AK019663; BAB31827.1; -; mRNA.
DR   EMBL; AK019802; BAB31856.1; -; mRNA.
DR   EMBL; BC025062; AAH25062.1; -; mRNA.
DR   CCDS; CCDS15557.1; -.
DR   RefSeq; NP_080902.1; NM_026626.2.
DR   AlphaFoldDB; Q9CQ46; -.
DR   SMR; Q9CQ46; -.
DR   STRING; 10090.ENSMUSP00000027775; -.
DR   iPTMnet; Q9CQ46; -.
DR   PhosphoSitePlus; Q9CQ46; -.
DR   EPD; Q9CQ46; -.
DR   PaxDb; Q9CQ46; -.
DR   PRIDE; Q9CQ46; -.
DR   ProteomicsDB; 277727; -.
DR   Antibodypedia; 34718; 27 antibodies from 11 providers.
DR   DNASU; 68226; -.
DR   Ensembl; ENSMUST00000027775; ENSMUSP00000027775; ENSMUSG00000026495.
DR   GeneID; 68226; -.
DR   KEGG; mmu:68226; -.
DR   UCSC; uc007dve.1; mouse.
DR   CTD; 84288; -.
DR   MGI; MGI:1915476; Efcab2.
DR   VEuPathDB; HostDB:ENSMUSG00000026495; -.
DR   eggNOG; KOG0027; Eukaryota.
DR   GeneTree; ENSGT00940000160590; -.
DR   HOGENOM; CLU_061288_19_2_1; -.
DR   InParanoid; Q9CQ46; -.
DR   OMA; RDYISMM; -.
DR   OrthoDB; 1096220at2759; -.
DR   PhylomeDB; Q9CQ46; -.
DR   TreeFam; TF324069; -.
DR   BioGRID-ORCS; 68226; 2 hits in 72 CRISPR screens.
DR   ChiTaRS; Efcab2; mouse.
DR   PRO; PR:Q9CQ46; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; Q9CQ46; protein.
DR   Bgee; ENSMUSG00000026495; Expressed in seminiferous tubule of testis and 200 other tissues.
DR   Genevisible; Q9CQ46; MM.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR   GO; GO:0097228; C:sperm principal piece; IDA:MGI.
DR   GO; GO:0005509; F:calcium ion binding; IDA:MGI.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13499; EF-hand_7; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW   Reference proteome; Repeat.
FT   CHAIN           1..164
FT                   /note="Dynein regulatory complex protein 8"
FT                   /id="PRO_0000253545"
FT   DOMAIN          16..51
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          94..129
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   164 AA;  18929 MW;  C8AF058D07F53E1B CRC64;
     MAEERDAEGT EALIAELHKK IKDAFEVFDH ESNNTVDVRE IGTIIRSLGC CPTEGELHDF
     IAEIEEEEPT GYIRFEKFIP VMTRALVERR YRPAAEDILL RAFEVLDPAK RGFLTKDELV
     KYMTEEGEPF SQEEMEEMLS AAIDPESNTI NYRDYITMMV VDEN