DRC9_DANRE
ID DRC9_DANRE Reviewed; 346 AA.
AC A3KQH2;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Dynein regulatory complex protein 9 {ECO:0000250|UniProtKB:A8HQ54};
DE AltName: Full=IQ domain-containing protein G {ECO:0000303|PubMed:24787902};
GN Name=iqcg {ECO:0000312|ZFIN:ZDB-GENE-050419-109};
GN Synonyms=drc9 {ECO:0000250|UniProtKB:A8HQ54},
GN si:ch211-222m18.3 {ECO:0000312|ZFIN:ZDB-GENE-050419-109};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CALMODULIN AND
RP HSP70, IDENTIFICATION IN A COMPLEX WITH HSP70 AND CAMK4, DOMAIN, DISRUPTION
RP PHENOTYPE, TISSUE SPECIFICITY, AND MUTAGENESIS OF GLN-313.
RX PubMed=24787902; DOI=10.1038/ncomms4811;
RA Chen L.T., Liang W.X., Chen S., Li R.K., Tan J.L., Xu P.F., Luo L.F.,
RA Wang L., Yu S.H., Meng G., Li K.K., Liu T.X., Chen Z., Chen S.J.;
RT "Functional and molecular features of the calmodulin-interacting protein
RT IQCG required for haematopoiesis in zebrafish.";
RL Nat. Commun. 5:3811-3811(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-166.
RX PubMed=18629171; DOI=10.1002/cfg.418;
RA Li Y., Chia J.M., Bartfai R., Christoffels A., Yue G.H., Ding K., Ho M.Y.,
RA Hill J.A., Stupka E., Orban L.;
RT "Comparative analysis of the testis and ovary transcriptomes in zebrafish
RT by combining experimental and computational tools.";
RL Comp. Funct. Genomics 5:403-418(2004).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC), a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes (By similarity). Binds calmodulin when
CC cellular Ca(2+) levels are low and thereby contributes to the
CC regulation of calcium and calmodulin-dependent protein kinase IV
CC (camk4) activity; contributes to the regulation of camk4 signaling
CC cascades. Plays a role in the regulation of definitive hematopoiesis
CC via its effects on camk4 (PubMed:24787902).
CC {ECO:0000250|UniProtKB:A8HQ54, ECO:0000269|PubMed:24787902}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC) (By
CC similarity). Interacts (via IQ domain) with calmodulin when calcium
CC levels are low. Does not interact with calmodulin in the presence of
CC Ca(2+). Interacts with hsp70 and may form a complex with camk4 and
CC hsp70 (PubMed:24787902). {ECO:0000250|UniProtKB:A8HQ54,
CC ECO:0000269|PubMed:24787902}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q80W32}. Cell
CC projection, cilium, flagellum {ECO:0000250|UniProtKB:Q80W32}. Cell
CC projection, cilium {ECO:0000250|UniProtKB:Q80W32}. Cytoplasm,
CC cytoskeleton {ECO:0000250|UniProtKB:Q80W32}. Cytoplasm, cytoskeleton,
CC flagellum axoneme {ECO:0000250|UniProtKB:A8HQ54}.
CC -!- TISSUE SPECIFICITY: Detected in adult testis, and at lower levels in
CC brain, kidney and ovary. {ECO:0000269|PubMed:24787902}.
CC -!- DOMAIN: The IQ domain mediates interaction with calmodulin when
CC cellular Ca(2+) levels are low. {ECO:0000269|PubMed:24787902}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein leads to a
CC severe reduction in the size of the caudal hematopoietic tissue at 72
CC hpf, a strong reduction in the number of myb-positive hematopoietic
CC stem cells, and results in impaired definitive hematopoiesis. Contrary
CC to wild-type, the expression of monocyte, macrophage and lymphoblast
CC markers is strongly reduced. Likewise, hemoglobin levels are strongly
CC reduced, suggesting impaired erythropoiesis.
CC {ECO:0000269|PubMed:24787902}.
CC -!- SIMILARITY: Belongs to the DRC9 family. {ECO:0000305}.
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DR EMBL; BX649600; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX908394; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CO359834; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001333169.1; NM_001346240.1.
DR AlphaFoldDB; A3KQH2; -.
DR SMR; A3KQH2; -.
DR STRING; 7955.ENSDARP00000090196; -.
DR PaxDb; A3KQH2; -.
DR Ensembl; ENSDART00000175259; ENSDARP00000144131; ENSDARG00000068678.
DR GeneID; 100322600; -.
DR KEGG; dre:100322600; -.
DR CTD; 84223; -.
DR ZFIN; ZDB-GENE-050419-109; iqcg.
DR eggNOG; ENOG502QQR7; Eukaryota.
DR GeneTree; ENSGT00730000111263; -.
DR HOGENOM; CLU_052522_1_1_1; -.
DR OrthoDB; 1353340at2759; -.
DR PhylomeDB; A3KQH2; -.
DR PRO; PR:A3KQH2; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 18.
DR Bgee; ENSDARG00000068678; Expressed in testis and 17 other tissues.
DR ExpressionAtlas; A3KQH2; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0031514; C:motile cilium; IDA:ZFIN.
DR GO; GO:0036126; C:sperm flagellum; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0044782; P:cilium organization; IBA:GO_Central.
DR GO; GO:0060216; P:definitive hemopoiesis; IMP:ZFIN.
DR GO; GO:0007288; P:sperm axoneme assembly; IBA:GO_Central.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR042618; IQCG.
DR PANTHER; PTHR14871; PTHR14871; 1.
DR Pfam; PF00612; IQ; 1.
DR SMART; SM00015; IQ; 1.
DR PROSITE; PS50096; IQ; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Differentiation; Flagellum; Reference proteome.
FT CHAIN 1..346
FT /note="Dynein regulatory complex protein 9"
FT /id="PRO_0000436997"
FT DOMAIN 305..334
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 299..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..346
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 313
FT /note="Q->A: Loss of calmodulin binding."
FT /evidence="ECO:0000269|PubMed:24787902"
SQ SEQUENCE 346 AA; 40662 MW; 62E2ECD91BF3478E CRC64;
MTSVEELRAC ALLQDCADQL SVLGNIIRPG AETQQRTELH LKTAQLMAGS SLSNFSGELK
SQKHFKIQQT LLASDNLAKV QKDRQFVSDV INALLEELQK KNNFQSLFSA VAEERKKKAE
LLDIINREEE GRRQIKKLQK QLLDIRKEKT EECERLEEEV AILKDQVQDM RVRTNQQGKF
VKSCAEQLVY QESKHNSYKE NELEDEVKML QEKIEEEKNV HFETEAFLKQ QHANLKQKLQ
YWIHRYEKDM EEKEQEITAL QNKRNSSQTR IQDLSKKCKD MENVVIEDRI EKEHLRAQME
KEQREKNAAT KIQAWWRGTL VRKGPRSKKA DKSKKKDGKK GKKKRK
