DRC9_MOUSE
ID DRC9_MOUSE Reviewed; 419 AA.
AC Q80W32;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Dynein regulatory complex protein 9 {ECO:0000250|UniProtKB:A8HQ54};
DE AltName: Full=IQ domain-containing protein G;
GN Name=Iqcg; Synonyms=Drc9 {ECO:0000250|UniProtKB:A8HQ54};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, MISCELLANEOUS, AND TISSUE SPECIFICITY.
RX PubMed=24362311; DOI=10.1534/g3.113.009563;
RA Harris T.P., Schimenti K.J., Munroe R.J., Schimenti J.C.;
RT "IQ motif-containing G (Iqcg) is required for mouse spermiogenesis.";
RL G3 (Bethesda) 4:367-372(2014).
RN [3]
RP IDENTIFICATION IN A COMPLEX WITH CAMK4 AND HSP70.
RX PubMed=24787902; DOI=10.1038/ncomms4811;
RA Chen L.T., Liang W.X., Chen S., Li R.K., Tan J.L., Xu P.F., Luo L.F.,
RA Wang L., Yu S.H., Meng G., Li K.K., Liu T.X., Chen Z., Chen S.J.;
RT "Functional and molecular features of the calmodulin-interacting protein
RT IQCG required for haematopoiesis in zebrafish.";
RL Nat. Commun. 5:3811-3811(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP AND INTERACTION WITH CALMODULIN.
RX PubMed=24849454; DOI=10.1371/journal.pone.0098053;
RA Li R.K., Tan J.L., Chen L.T., Feng J.S., Liang W.X., Guo X.J., Liu P.,
RA Chen Z., Sha J.H., Wang Y.F., Chen S.J.;
RT "Iqcg is essential for sperm flagellum formation in mice.";
RL PLoS ONE 9:E98053-E98053(2014).
CC -!- FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC), a
CC key regulator of ciliary/flagellar motility which maintains the
CC alignment and integrity of the distal axoneme and regulates microtubule
CC sliding in motile axonemes. Binds calmodulin when cellular Ca(2+)
CC levels are low and thereby contributes to the regulation of calcium and
CC calmodulin-dependent protein kinase IV (CAMK4) activity; contributes to
CC the regulation of CAMK4 signaling cascades (By similarity). Required
CC for normal axoneme assembly in sperm flagella, normal sperm tail
CC formation and for male fertility (PubMed:24362311, PubMed:24849454).
CC {ECO:0000250|UniProtKB:A3KQH2, ECO:0000250|UniProtKB:A8HQ54,
CC ECO:0000269|PubMed:24362311, ECO:0000269|PubMed:24849454}.
CC -!- SUBUNIT: Component of the nexin-dynein regulatory complex (N-DRC) (By
CC similarity). Interacts (via IQ domain) with CALM when calcium levels
CC are low. Does not interact with CALM in the presence of Ca(2+)
CC (PubMed:24849454). Interacts with the HSP70 proteins HSPA1L and HSPA8
CC (By similarity). May form a complex with CAMK4 and HSP70 (Probable).
CC {ECO:0000250|UniProtKB:A8HQ54, ECO:0000250|UniProtKB:Q9H095,
CC ECO:0000269|PubMed:24849454, ECO:0000305|PubMed:24787902}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24849454}. Cell
CC projection, cilium, flagellum {ECO:0000269|PubMed:24849454}. Cell
CC projection, cilium {ECO:0000269|PubMed:24849454}. Cytoplasm,
CC cytoskeleton {ECO:0000269|PubMed:24849454}. Cytoplasm, cytoskeleton,
CC flagellum axoneme {ECO:0000250|UniProtKB:A8HQ54}. Note=First detected
CC in the cytoplasm of pachytene spermatocytes. Colocalizes with alpha-
CC tubulin at the manchette in developing spermatids. Detected in the
CC flagellum of mature testicular spermatozoa, and in the flagellum and
CC post-acrosomal region of the head of epididymal spermatozoa. Detected
CC in cilia in trachea and oviduct. {ECO:0000269|PubMed:24849454}.
CC -!- TISSUE SPECIFICITY: Detected in testis (PubMed:24362311,
CC PubMed:24849454). Also detected in oviduct (at protein level)
CC (PubMed:24849454). Detected in testis (PubMed:24362311,
CC PubMed:24849454). Also detected in oviduct and trachea
CC (PubMed:24849454). {ECO:0000269|PubMed:24362311,
CC ECO:0000269|PubMed:24849454}.
CC -!- DEVELOPMENTAL STAGE: First detected in testis 17 days after birth when
CC pachytene spermatocytes are seen in testes. Expression remains high
CC during the first three weeks after birth and in adults (at protein
CC level). {ECO:0000269|PubMed:24362311}.
CC -!- DOMAIN: The IQ domain mediates interaction with calmodulin when
CC cellular Ca(2+) levels are low. {ECO:0000250|UniProtKB:Q9H095}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotype, except complete male
CC sterility (PubMed:24362311, PubMed:24849454). Female fertility is not
CC altered (PubMed:24849454). {ECO:0000269|PubMed:24362311,
CC ECO:0000269|PubMed:24849454}.
CC -!- MISCELLANEOUS: Early spermiogenesis defective 12d (esgd12d) is caused
CC by mutations disrupting this gene. Affected male mice display complete
CC loss of fertility; their sperm cells lack tails and are nonmotile.
CC {ECO:0000269|PubMed:24362311}.
CC -!- SIMILARITY: Belongs to the DRC9 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC049990; AAH49990.1; -; mRNA.
DR CCDS; CCDS37316.1; -.
DR RefSeq; NP_848465.1; NM_178378.3.
DR RefSeq; XP_011244306.1; XM_011246004.2.
DR AlphaFoldDB; Q80W32; -.
DR SMR; Q80W32; -.
DR STRING; 10090.ENSMUSP00000110752; -.
DR iPTMnet; Q80W32; -.
DR PhosphoSitePlus; Q80W32; -.
DR jPOST; Q80W32; -.
DR PaxDb; Q80W32; -.
DR PRIDE; Q80W32; -.
DR ProteomicsDB; 301666; -.
DR Antibodypedia; 51727; 75 antibodies from 11 providers.
DR DNASU; 69707; -.
DR Ensembl; ENSMUST00000115100; ENSMUSP00000110752; ENSMUSG00000035578.
DR GeneID; 69707; -.
DR KEGG; mmu:69707; -.
DR UCSC; uc007yzt.2; mouse.
DR CTD; 84223; -.
DR MGI; MGI:1916957; Iqcg.
DR VEuPathDB; HostDB:ENSMUSG00000035578; -.
DR eggNOG; ENOG502QQR7; Eukaryota.
DR GeneTree; ENSGT00730000111263; -.
DR HOGENOM; CLU_052522_0_0_1; -.
DR InParanoid; Q80W32; -.
DR OMA; IMNTETL; -.
DR OrthoDB; 1353340at2759; -.
DR PhylomeDB; Q80W32; -.
DR TreeFam; TF326203; -.
DR BioGRID-ORCS; 69707; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Iqcg; mouse.
DR PRO; PR:Q80W32; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q80W32; protein.
DR Bgee; ENSMUSG00000035578; Expressed in spermatocyte and 88 other tissues.
DR Genevisible; Q80W32; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0002177; C:manchette; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0005516; F:calmodulin binding; IDA:UniProtKB.
DR GO; GO:0030544; F:Hsp70 protein binding; ISO:MGI.
DR GO; GO:0044782; P:cilium organization; IBA:GO_Central.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:UniProtKB.
DR GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR042618; IQCG.
DR PANTHER; PTHR14871; PTHR14871; 1.
DR Pfam; PF00612; IQ; 1.
DR PROSITE; PS50096; IQ; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Differentiation; Flagellum; Reference proteome; Spermatogenesis.
FT CHAIN 1..419
FT /note="Dynein regulatory complex protein 9"
FT /id="PRO_0000282563"
FT DOMAIN 372..401
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 25..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 394..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..45
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 394..411
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 419 AA; 49168 MW; C38867360202E88E CRC64;
MDEEEVEVVE SPSEVLQPEV TVVVTGEPPE AAEEDLDYEE EEETSPEVIE TLSLLDVLRV
SAVMEDVIDQ LSILGYIIPV QYERRQSLTQ KASHEGASMI TTTPKKSTSL LTKEKSMMAE
NKQRGQDFTF KKPTKQTMMT LETLKKIQND RQYFSDVIAN AMMEMQDSGS FTSLLKALGK
ERDSKMNFHD VITREEKGRK QIKTLQKQLL DVKRERQMQV QNGNEYIAHL RDQLQEMKAK
TNLENLYMKR NAELQISQTQ KKCNRAEELL LEEIEKLRMK TEEENRVHTE IEMFLKKQQQ
KLEEKLEFWM EKFDKDTEAK QNELNALKAA KASDLVHLQD LAKMIREYEQ VIIEDRIEKE
KTRKKLEQDD LELRSIVKLQ AWWRGSVVRK EIGNFKMPKK DKDDSKDSKG KEKEKRRKK
