DRD2L_DROME
ID DRD2L_DROME Reviewed; 905 AA.
AC Q8IS44; Q4ABK5; Q59E80; Q59E81; Q59E82; Q6AWL2; Q8IS43; Q8IS45; Q9W5X7;
AC Q9W5X8; X2JLE1;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Dopamine D2-like receptor;
DE Short=DD2R;
GN Name=Dop2R {ECO:0000312|FlyBase:FBgn0053517}; Synonyms=D2R;
GN ORFNames=CG17004 {ECO:0000312|FlyBase:FBgn0053517};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAN15955.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 238-905 (ISOFORMS H; F AND J), FUNCTION,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Head {ECO:0000269|PubMed:12391323};
RX PubMed=12391323; DOI=10.1073/pnas.202498299;
RA Hearn M.G., Ren Y., McBride E.W., Reveillaud I., Beinborn M., Kopin A.S.;
RT "A Drosophila dopamine 2-like receptor: molecular characterization and
RT identification of multiple alternatively spliced variants.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14554-14559(2002).
CC -!- FUNCTION: Receptor for dopamine. The activity of this receptor is
CC mediated by G proteins which inhibit adenylyl cyclase.
CC {ECO:0000269|PubMed:12391323}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12391323};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=H {ECO:0000312|FlyBase:FBgn0053517}; Synonyms=606
CC {ECO:0000303|PubMed:12391323}, DD2R-606 {ECO:0000303|PubMed:12391323};
CC IsoId=Q8IS44-1; Sequence=Displayed;
CC Name=J {ECO:0000312|FlyBase:FBgn0053517}; Synonyms=506
CC {ECO:0000303|PubMed:12391323}, DD2R-506 {ECO:0000303|PubMed:12391323};
CC IsoId=Q8IS44-2; Sequence=VSP_059318;
CC Name=F {ECO:0000312|FlyBase:FBgn0053517}; Synonyms=461
CC {ECO:0000303|PubMed:12391323}, DD2R-461 {ECO:0000303|PubMed:12391323};
CC IsoId=Q8IS44-3; Sequence=VSP_059317;
CC Name=B {ECO:0000312|FlyBase:FBgn0053517};
CC IsoId=Q8IS44-4; Sequence=VSP_059316, VSP_059320;
CC Name=G {ECO:0000312|FlyBase:FBgn0053517};
CC IsoId=Q8IS44-5; Sequence=VSP_059320;
CC Name=I {ECO:0000312|FlyBase:FBgn0053517};
CC IsoId=Q8IS44-6; Sequence=VSP_059319;
CC -!- TISSUE SPECIFICITY: Highest expression is in adult heads.
CC {ECO:0000269|PubMed:12391323}.
CC -!- DEVELOPMENTAL STAGE: Expressed in larva, pupae and adult.
CC {ECO:0000269|PubMed:12391323}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN15955.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAN15956.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAN15957.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAT94465.2; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY150862; AAN15955.1; ALT_INIT; mRNA.
DR EMBL; AY150863; AAN15956.1; ALT_INIT; mRNA.
DR EMBL; AY150864; AAN15957.1; ALT_INIT; mRNA.
DR EMBL; AE014298; AAF45375.2; -; Genomic_DNA.
DR EMBL; AE014298; AAX52462.1; -; Genomic_DNA.
DR EMBL; AE014298; AAX52463.1; -; Genomic_DNA.
DR EMBL; AE014298; AAX52464.1; -; Genomic_DNA.
DR EMBL; AE014298; AAZ52487.2; -; Genomic_DNA.
DR EMBL; AE014298; AHN59947.1; -; Genomic_DNA.
DR EMBL; BT015236; AAT94465.2; ALT_FRAME; mRNA.
DR RefSeq; NP_001014757.2; NM_001014757.3. [Q8IS44-3]
DR RefSeq; NP_001014758.2; NM_001014758.3. [Q8IS44-1]
DR RefSeq; NP_001014759.1; NM_001014759.2. [Q8IS44-4]
DR RefSeq; NP_001014760.2; NM_001014760.3. [Q8IS44-6]
DR RefSeq; NP_001027080.2; NM_001031909.2. [Q8IS44-2]
DR RefSeq; NP_001285477.1; NM_001298548.1. [Q8IS44-5]
DR AlphaFoldDB; Q8IS44; -.
DR SMR; Q8IS44; -.
DR BioGRID; 59301; 2.
DR STRING; 7227.FBpp0099564; -.
DR GlyGen; Q8IS44; 8 sites.
DR PaxDb; Q8IS44; -.
DR EnsemblMetazoa; FBtr0070034; FBpp0099563; FBgn0053517. [Q8IS44-4]
DR EnsemblMetazoa; FBtr0343167; FBpp0309861; FBgn0053517. [Q8IS44-3]
DR EnsemblMetazoa; FBtr0343168; FBpp0309862; FBgn0053517. [Q8IS44-5]
DR EnsemblMetazoa; FBtr0343169; FBpp0309863; FBgn0053517. [Q8IS44-1]
DR EnsemblMetazoa; FBtr0343170; FBpp0309864; FBgn0053517. [Q8IS44-6]
DR EnsemblMetazoa; FBtr0343171; FBpp0309865; FBgn0053517. [Q8IS44-2]
DR GeneID; 33007; -.
DR KEGG; dme:Dmel_CG33517; -.
DR CTD; 33007; -.
DR FlyBase; FBgn0053517; Dop2R.
DR VEuPathDB; VectorBase:FBgn0053517; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000164988; -.
DR InParanoid; Q8IS44; -.
DR OMA; CHVIPND; -.
DR PhylomeDB; Q8IS44; -.
DR Reactome; R-DME-390651; Dopamine receptors.
DR Reactome; R-DME-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 33007; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33007; -.
DR PRO; PR:Q8IS44; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0053517; Expressed in brain and 8 other tissues.
DR ExpressionAtlas; Q8IS44; baseline and differential.
DR Genevisible; Q8IS44; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0001591; F:dopamine neurotransmitter receptor activity, coupled via Gi/Go; IDA:UniProtKB.
DR GO; GO:0008227; F:G protein-coupled amine receptor activity; ISS:FlyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007615; P:anesthesia-resistant memory; IMP:FlyBase.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..905
FT /note="Dopamine D2-like receptor"
FT /id="PRO_0000069395"
FT TOPO_DOM 1..377
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 378..398
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 417..437
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..450
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 451..471
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..493
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 494..514
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..531
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 532..552
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 553..830
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 831..851
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 852..869
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 870..890
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 891..905
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 600..631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..753
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 780..799
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..735
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 257
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 314
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 343
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 448..525
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 1..436
FT /note="MLSPFDWRRGISSSGTGGTMAAQPLSSTAATTAAATGATAATAATAATTSAT
FT LSTAAASTSTTAAPSAGATWINHHLAVEADSSQPANGSDAQAGVEGPTMPAGYLPLYED
FT VETAAEDAGYALIDDISEWLLGSVGSEAAVGGPENSTNLAVTGANGTLAWLEALNSTQP
FT AQSNSSAEDGERGRYSLRSFVEQQLAGGGAAGAGDGGDAGIALIDSGEEAALDNVADAE
FT TDYGMLGGFGDAELLQRTATVARETLGNRTAPSTTSYDGGGSGDVGVAGGLAGTAGGGV
FT GGAGGSGGSTFMLLLENFNDYFPNYNGSTVSGTSTIAPGVAITGSRGSGLLLEQNLTGL
FT YLDGYRLNCTNETLNLTDSCGELRVVDHNYWALILILFPILTLFGNILVILSVCRERSL
FT QTVTNYFIVSLAIADLLVAVVVMPFAVYFL -> MSAPK (in isoform B)"
FT /id="VSP_059316"
FT VAR_SEQ 657..802
FT /note="NSVVAQITTQPQLVVADPNGNHDSGYAASNVDDVLAGVAPASASAATSAAPR
FT SSGSPPDSPLPSGATLQRSSVSSQRRPTGDDSPKRGEPALRSVGVDNSSVAMKPLSFVR
FT YGVQEAMTLARNDSTLSTTSKTSSRKDKKNSQASR -> K (in isoform F)"
FT /id="VSP_059317"
FT VAR_SEQ 658..757
FT /note="Missing (in isoform J)"
FT /id="VSP_059318"
FT VAR_SEQ 658
FT /note="Missing (in isoform I)"
FT /id="VSP_059319"
FT VAR_SEQ 749..756
FT /note="Missing (in isoform G and isoform B)"
FT /id="VSP_059320"
FT CONFLICT 739
FT /note="D -> E (in Ref. 1; AAN15955)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 905 AA; 95057 MW; A1973196A9AD4B9C CRC64;
MLSPFDWRRG ISSSGTGGTM AAQPLSSTAA TTAAATGATA ATAATAATTS ATLSTAAAST
STTAAPSAGA TWINHHLAVE ADSSQPANGS DAQAGVEGPT MPAGYLPLYE DVETAAEDAG
YALIDDISEW LLGSVGSEAA VGGPENSTNL AVTGANGTLA WLEALNSTQP AQSNSSAEDG
ERGRYSLRSF VEQQLAGGGA AGAGDGGDAG IALIDSGEEA ALDNVADAET DYGMLGGFGD
AELLQRTATV ARETLGNRTA PSTTSYDGGG SGDVGVAGGL AGTAGGGVGG AGGSGGSTFM
LLLENFNDYF PNYNGSTVSG TSTIAPGVAI TGSRGSGLLL EQNLTGLYLD GYRLNCTNET
LNLTDSCGEL RVVDHNYWAL ILILFPILTL FGNILVILSV CRERSLQTVT NYFIVSLAIA
DLLVAVVVMP FAVYFLVNGA WALPDVVCDF YIAMDVICST SSIFNLVAIS IDRYIAVTQP
IKYAKHKNSR RVCLTILLVW AISAAIGSPI VLGLNNTPNR EPDVCAFYNA DFILYSSLSS
FYIPCIIMVF LYWNIFKALR SRARKQRAAR KPHLSELTGG SVIENIAQTR RLAETALDSS
RHASRILPDE AATNTASGSN EEEDENAISP DIDDCHVIVN DKSTEFMLAT VVEETGNSVV
AQITTQPQLV VADPNGNHDS GYAASNVDDV LAGVAPASAS AATSAAPRSS GSPPDSPLPS
GATLQRSSVS SQRRPTGDDS PKRGEPALRS VGVDNSSVAM KPLSFVRYGV QEAMTLARND
STLSTTSKTS SRKDKKNSQA SRFTIYKVHK ASKKKREKSS AKKERKATKT LAIVLGVFLF
CWLPFFSCNI MDAMCAKFKK DCRPGLTAYM MTTWLGYINS FVNPVIYTIF NPEFRKAFKK
IMHMG
