DRD3_MOUSE
ID DRD3_MOUSE Reviewed; 446 AA.
AC P30728;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=D(3) dopamine receptor;
DE AltName: Full=Dopamine D3 receptor;
GN Name=Drd3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D3LONG AND D3SHORT).
RC STRAIN=BALB/cJ;
RX PubMed=8449953; DOI=10.1016/s0021-9258(18)53401-3;
RA Fishburn C.S., Belleli D., David C., Carmon S., Fuchs S.;
RT "A novel short isoform of the D3 dopamine receptor generated by alternative
RT splicing in the third cytoplasmic loop.";
RL J. Biol. Chem. 268:5872-5878(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7598803; DOI=10.1089/dna.1995.14.485;
RA Fu D., Skryabin B.V., Brosius J., Robakis N.K.;
RT "Molecular cloning and characterization of the mouse dopamine D3 receptor
RT gene: an additional intron and an mRNA variant.";
RL DNA Cell Biol. 14:485-492(1995).
CC -!- FUNCTION: Dopamine receptor whose activity is mediated by G proteins
CC which inhibit adenylyl cyclase. Promotes cell proliferation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CLIC6 (By similarity). Interacts with GRK4.
CC Interacts with PALM. Interacts with FLNA (via filamin repeat 21);
CC increases PKA-mediated phosphorylation of FLNA (By similarity).
CC {ECO:0000250|UniProtKB:P19020, ECO:0000250|UniProtKB:P35462}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=D3Long;
CC IsoId=P30728-1; Sequence=Displayed;
CC Name=D3Short;
CC IsoId=P30728-2; Sequence=VSP_001873;
CC -!- PTM: Phosphorylated by GRK4. {ECO:0000250|UniProtKB:P35462}.
CC -!- PTM: Palmitoylated. {ECO:0000250|UniProtKB:P35462}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; X67274; CAA47691.1; -; mRNA.
DR EMBL; S78580; AAB35066.2; -; Genomic_DNA.
DR EMBL; S78574; AAB35066.2; JOINED; Genomic_DNA.
DR EMBL; S78638; AAB35066.2; JOINED; Genomic_DNA.
DR EMBL; S78577; AAB35066.2; JOINED; Genomic_DNA.
DR EMBL; S78578; AAB35066.2; JOINED; Genomic_DNA.
DR EMBL; S78579; AAB35066.2; JOINED; Genomic_DNA.
DR EMBL; S78639; AAB35066.2; JOINED; Genomic_DNA.
DR CCDS; CCDS28179.1; -. [P30728-1]
DR PIR; I48322; I48322.
DR RefSeq; NP_031903.1; NM_007877.2. [P30728-1]
DR AlphaFoldDB; P30728; -.
DR SMR; P30728; -.
DR BioGRID; 199307; 2.
DR CORUM; P30728; -.
DR STRING; 10090.ENSMUSP00000023390; -.
DR BindingDB; P30728; -.
DR ChEMBL; CHEMBL3441; -.
DR DrugCentral; P30728; -.
DR GlyGen; P30728; 4 sites.
DR PhosphoSitePlus; P30728; -.
DR PaxDb; P30728; -.
DR PRIDE; P30728; -.
DR ProteomicsDB; 279577; -. [P30728-1]
DR ProteomicsDB; 279578; -. [P30728-2]
DR Antibodypedia; 16482; 284 antibodies from 38 providers.
DR DNASU; 13490; -.
DR Ensembl; ENSMUST00000023390; ENSMUSP00000023390; ENSMUSG00000022705. [P30728-1]
DR GeneID; 13490; -.
DR KEGG; mmu:13490; -.
DR UCSC; uc007zgi.1; mouse. [P30728-1]
DR CTD; 1814; -.
DR MGI; MGI:94925; Drd3.
DR VEuPathDB; HostDB:ENSMUSG00000022705; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000159661; -.
DR HOGENOM; CLU_009579_11_1_1; -.
DR InParanoid; P30728; -.
DR OMA; YYSICQG; -.
DR OrthoDB; 384993at2759; -.
DR PhylomeDB; P30728; -.
DR TreeFam; TF334382; -.
DR Reactome; R-MMU-390651; Dopamine receptors.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 13490; 4 hits in 73 CRISPR screens.
DR PRO; PR:P30728; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; P30728; protein.
DR Bgee; ENSMUSG00000022705; Expressed in morula and 53 other tissues.
DR ExpressionAtlas; P30728; baseline and differential.
DR Genevisible; P30728; MM.
DR GO; GO:0045177; C:apical part of cell; ISO:MGI.
DR GO; GO:0042995; C:cell projection; ISO:MGI.
DR GO; GO:0098691; C:dopaminergic synapse; IDA:SynGO.
DR GO; GO:0030139; C:endocytic vesicle; ISO:MGI.
DR GO; GO:0098982; C:GABA-ergic synapse; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:MGI.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0031748; F:D1 dopamine receptor binding; ISO:MGI.
DR GO; GO:0001591; F:dopamine neurotransmitter receptor activity, coupled via Gi/Go; IDA:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0019904; F:protein domain specific binding; ISO:MGI.
DR GO; GO:0046717; P:acid secretion; ISO:MGI.
DR GO; GO:0071880; P:adenylate cyclase-activating adrenergic receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007191; P:adenylate cyclase-activating dopamine receptor signaling pathway; IDA:MGI.
DR GO; GO:0007195; P:adenylate cyclase-inhibiting dopamine receptor signaling pathway; ISO:MGI.
DR GO; GO:0050482; P:arachidonic acid secretion; ISO:MGI.
DR GO; GO:0006914; P:autophagy; ISO:MGI.
DR GO; GO:0048148; P:behavioral response to cocaine; IMP:BHF-UCL.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISO:MGI.
DR GO; GO:0032922; P:circadian regulation of gene expression; IMP:MGI.
DR GO; GO:0007212; P:dopamine receptor signaling pathway; IMP:MGI.
DR GO; GO:0002031; P:G protein-coupled receptor internalization; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0035483; P:gastric emptying; ISO:MGI.
DR GO; GO:0007626; P:locomotory behavior; IMP:MGI.
DR GO; GO:0050883; P:musculoskeletal movement, spinal reflex action; IMP:MGI.
DR GO; GO:0007194; P:negative regulation of adenylate cyclase activity; IBA:GO_Central.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0045776; P:negative regulation of blood pressure; IMP:MGI.
DR GO; GO:0051481; P:negative regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0060160; P:negative regulation of dopamine receptor signaling pathway; IGI:MGI.
DR GO; GO:0048715; P:negative regulation of oligodendrocyte differentiation; IDA:MGI.
DR GO; GO:0051898; P:negative regulation of protein kinase B signaling; IMP:MGI.
DR GO; GO:0050709; P:negative regulation of protein secretion; ISO:MGI.
DR GO; GO:0032416; P:negative regulation of sodium:proton antiporter activity; ISO:MGI.
DR GO; GO:0051967; P:negative regulation of synaptic transmission, glutamatergic; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:1901386; P:negative regulation of voltage-gated calcium channel activity; IBA:GO_Central.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IDA:MGI.
DR GO; GO:0060158; P:phospholipase C-activating dopamine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:MGI.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IBA:GO_Central.
DR GO; GO:0060161; P:positive regulation of dopamine receptor signaling pathway; IMP:MGI.
DR GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISO:MGI.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0060134; P:prepulse inhibition; ISO:MGI.
DR GO; GO:0043491; P:protein kinase B signaling; IMP:MGI.
DR GO; GO:0002016; P:regulation of blood volume by renin-angiotensin; IMP:MGI.
DR GO; GO:0045187; P:regulation of circadian sleep/wake cycle, sleep; ISO:MGI.
DR GO; GO:0014059; P:regulation of dopamine secretion; IMP:MGI.
DR GO; GO:0019216; P:regulation of lipid metabolic process; IMP:MGI.
DR GO; GO:0040012; P:regulation of locomotion; ISO:MGI.
DR GO; GO:0090325; P:regulation of locomotion involved in locomotory behavior; ISO:MGI.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IMP:MGI.
DR GO; GO:0051580; P:regulation of neurotransmitter uptake; IDA:SynGO.
DR GO; GO:0099149; P:regulation of postsynaptic neurotransmitter receptor internalization; ISO:MGI.
DR GO; GO:0043266; P:regulation of potassium ion transport; IBA:GO_Central.
DR GO; GO:0001975; P:response to amphetamine; IGI:MGI.
DR GO; GO:0042220; P:response to cocaine; IMP:MGI.
DR GO; GO:0045471; P:response to ethanol; IMP:MGI.
DR GO; GO:0034776; P:response to histamine; ISO:MGI.
DR GO; GO:0043278; P:response to morphine; IMP:MGI.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:MGI.
DR GO; GO:0008542; P:visual learning; IMP:MGI.
DR InterPro; IPR001620; Dopamine_D3_rcpt.
DR InterPro; IPR000929; Dopamine_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00568; DOPAMINED3R.
DR PRINTS; PR00242; DOPAMINER.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..446
FT /note="D(3) dopamine receptor"
FT /id="PRO_0000069398"
FT TOPO_DOM 1..32
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 33..55
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 56..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 66..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 89..104
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 105..126
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 127..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 150..170
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 171..187
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 188..209
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 210..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 376..397
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 398..412
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TRANSMEM 413..432
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT TOPO_DOM 433..446
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P35462"
FT CARBOHYD 12
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 103..181
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT DISULFID 401..404
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 268..288
FT /note="Missing (in isoform D3Short)"
FT /evidence="ECO:0000303|PubMed:8449953"
FT /id="VSP_001873"
SQ SEQUENCE 446 AA; 49669 MW; B391C2D493B58242 CRC64;
MAPLSQISSH INSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL
QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN
LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTGDPSI
CSISNPDFVI YSSVVSFYVP FGVTVLVYAR IYMVLRQRRR KRILTRQNSQ CISIRPGFPQ
QSSCLRLHPI RQFSIRARFL SDATGQMEHI EDKPYPQKCQ DPLLSHLQPL SPGQTHGELK
RYYSICQDTA LRHPNFEGGG GMSQVERTRN SLSPTMAPKL SLEVRKLSNG RLSTSLKLGP
LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH CQACHVSPEL YRATTWLGYV
NSALNPVIYT TFNIEFRKAF LKILSC
