DRE2B_ARATH
ID DRE2B_ARATH Reviewed; 330 AA.
AC O82133;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Dehydration-responsive element-binding protein 2B;
DE Short=Protein DREB2B;
GN Name=DREB2B; Synonyms=ERF044; OrderedLocusNames=At3g11020;
GN ORFNames=F9F8.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=9707537; DOI=10.2307/3870648;
RA Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K.,
RA Shinozaki K.;
RT "Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding
RT domain separate two cellular signal transduction pathways in drought- and
RT low-temperature-responsive gene expression, respectively, in Arabidopsis.";
RL Plant Cell 10:1391-1406(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10809011; DOI=10.1023/a:1006321900483;
RA Nakashima K., Shinwari Z.K., Sakuma Y., Seki M., Miura S., Shinozaki K.,
RA Yamaguchi-Shinozaki K.;
RT "Organization and expression of two Arabidopsis DREB2 genes encoding DRE-
RT binding proteins involved in dehydration- and high-salinity-responsive gene
RT expression.";
RL Plant Mol. Biol. 42:657-665(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP GENE FAMILY, AND FUNCTION.
RX PubMed=11798174; DOI=10.1006/bbrc.2001.6299;
RA Sakuma Y., Liu Q., Dubouzet J.G., Abe H., Shinozaki K.,
RA Yamaguchi-Shinozaki K.;
RT "DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs,
RT transcription factors involved in dehydration- and cold-inducible gene
RT expression.";
RL Biochem. Biophys. Res. Commun. 290:998-1009(2002).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates
CC high salinity- and dehydration-inducible transcription.
CC {ECO:0000269|PubMed:11798174}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed preferentially in roots and stems, and at
CC a lower level in leaves. {ECO:0000269|PubMed:10809011}.
CC -!- INDUCTION: By high-salt and drought stresses.
CC {ECO:0000269|PubMed:10809011, ECO:0000269|PubMed:9707537}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AB007791; BAA33795.1; -; mRNA.
DR EMBL; AB016571; BAA36706.1; -; Genomic_DNA.
DR EMBL; AC009991; AAF01519.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74994.1; -; Genomic_DNA.
DR EMBL; AK117363; BAC42033.1; -; mRNA.
DR EMBL; BT008351; AAP37710.1; -; mRNA.
DR PIR; T51834; T51834.
DR RefSeq; NP_187713.1; NM_111939.3.
DR AlphaFoldDB; O82133; -.
DR SMR; O82133; -.
DR BioGRID; 5607; 4.
DR IntAct; O82133; 4.
DR STRING; 3702.AT3G11020.1; -.
DR PaxDb; O82133; -.
DR PRIDE; O82133; -.
DR EnsemblPlants; AT3G11020.1; AT3G11020.1; AT3G11020.
DR GeneID; 820273; -.
DR Gramene; AT3G11020.1; AT3G11020.1; AT3G11020.
DR KEGG; ath:AT3G11020; -.
DR Araport; AT3G11020; -.
DR TAIR; locus:2085527; AT3G11020.
DR eggNOG; ENOG502QTBU; Eukaryota.
DR HOGENOM; CLU_046486_0_1_1; -.
DR InParanoid; O82133; -.
DR OMA; CVVASEF; -.
DR OrthoDB; 1404403at2759; -.
DR PhylomeDB; O82133; -.
DR PRO; PR:O82133; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O82133; baseline and differential.
DR Genevisible; O82133; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010286; P:heat acclimation; IEP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; Coiled coil; DNA-binding; Nucleus; Reference proteome;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..330
FT /note="Dehydration-responsive element-binding protein 2B"
FT /id="PRO_0000112535"
FT DNA_BIND 77..134
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 46..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 230..247
FT /evidence="ECO:0000255"
FT MOTIF 13..54
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 9..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 292..306
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 330 AA; 37117 MW; 41405E07EB000CA3 CRC64;
MAVYEQTGTE QPKKRKSRAR AGGLTVADRL KKWKEYNEIV EASAVKEGEK PKRKVPAKGS
KKGCMKGKGG PDNSHCSFRG VRQRIWGKWV AEIREPKIGT RLWLGTFPTA EKAASAYDEA
ATAMYGSLAR LNFPQSVGSE FTSTSSQSEV CTVENKAVVC GDVCVKHEDT DCESNPFSQI
LDVREESCGT RPDSCTVGHQ DMNSSLNYDL LLEFEQQYWG QVLQEKEKPK QEEEEIQQQQ
QEQQQQQLQP DLLTVADYGW PWSNDIVNDQ TSWDPNECFD INELLGDLNE PGPHQSQDQN
HVNSGSYDLH PLHLEPHDGH EFNGLSSLDI
