DRG2_ARATH
ID DRG2_ARATH Reviewed; 399 AA.
AC Q9CAI1; C0Z340; Q56Z00;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Developmentally-regulated G-protein 2;
DE Short=AtDRG2;
DE AltName: Full=Developmentally-regulated G-protein 2B;
DE Short=AtDRG2b;
GN Name=DRG2; Synonyms=DRG2B; OrderedLocusNames=At1g72660; ORFNames=F28P22.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 239-399 (ISOFORM 1/2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=19460440; DOI=10.1016/j.pep.2009.05.009;
RA O'Connell A., Robin G., Kobe B., Botella J.R.;
RT "Biochemical characterization of Arabidopsis developmentally regulated G-
RT proteins (DRGs).";
RL Protein Expr. Purif. 67:88-95(2009).
CC -!- FUNCTION: Binds GDP and GTP, and has low GTPase activity.
CC {ECO:0000269|PubMed:19460440}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CAI1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CAI1-2; Sequence=VSP_041722;
CC -!- SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase
CC superfamily. OBG GTPase family. {ECO:0000255|PROSITE-ProRule:PRU01047}.
CC -!- CAUTION: The nomenclature of the 3 Arabidopsis DRG genes is ambiguous;
CC in the literature several gene names have been used for the same
CC protein. {ECO:0000305}.
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DR EMBL; AC010926; AAG51856.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35356.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35357.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35358.1; -; Genomic_DNA.
DR EMBL; AK118905; BAC43489.1; -; mRNA.
DR EMBL; BT006031; AAP04018.1; -; mRNA.
DR EMBL; AK319004; BAH57119.1; -; mRNA.
DR EMBL; AK221170; BAD95228.1; -; mRNA.
DR PIR; C96751; C96751.
DR RefSeq; NP_001031270.1; NM_001036193.2. [Q9CAI1-1]
DR RefSeq; NP_001031271.1; NM_001036194.1. [Q9CAI1-1]
DR RefSeq; NP_177410.1; NM_105925.4. [Q9CAI1-1]
DR AlphaFoldDB; Q9CAI1; -.
DR SMR; Q9CAI1; -.
DR BioGRID; 28817; 1.
DR STRING; 3702.AT1G72660.2; -.
DR PaxDb; Q9CAI1; -.
DR PRIDE; Q9CAI1; -.
DR ProteomicsDB; 224302; -. [Q9CAI1-1]
DR EnsemblPlants; AT1G72660.1; AT1G72660.1; AT1G72660. [Q9CAI1-1]
DR EnsemblPlants; AT1G72660.2; AT1G72660.2; AT1G72660. [Q9CAI1-1]
DR EnsemblPlants; AT1G72660.3; AT1G72660.3; AT1G72660. [Q9CAI1-1]
DR GeneID; 843598; -.
DR Gramene; AT1G72660.1; AT1G72660.1; AT1G72660. [Q9CAI1-1]
DR Gramene; AT1G72660.2; AT1G72660.2; AT1G72660. [Q9CAI1-1]
DR Gramene; AT1G72660.3; AT1G72660.3; AT1G72660. [Q9CAI1-1]
DR KEGG; ath:AT1G72660; -.
DR Araport; AT1G72660; -.
DR TAIR; locus:2030240; AT1G72660.
DR eggNOG; KOG1486; Eukaryota.
DR HOGENOM; CLU_044997_0_0_1; -.
DR InParanoid; Q9CAI1; -.
DR OMA; VCDQVHR; -.
DR OrthoDB; 754662at2759; -.
DR PhylomeDB; Q9CAI1; -.
DR PRO; PR:Q9CAI1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAI1; baseline and differential.
DR Genevisible; Q9CAI1; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0019003; F:GDP binding; IDA:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0002181; P:cytoplasmic translation; IBA:GO_Central.
DR CDD; cd01896; DRG; 1.
DR Gene3D; 3.10.20.30; -; 1.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR045001; DRG.
DR InterPro; IPR031167; G_OBG.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR031662; GTP-binding_2.
DR InterPro; IPR006074; GTP1-OBG_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR004095; TGS.
DR InterPro; IPR012676; TGS-like.
DR PANTHER; PTHR43127; PTHR43127; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR Pfam; PF16897; MMR_HSR1_Xtn; 1.
DR Pfam; PF02824; TGS; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF81271; SSF81271; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51710; G_OBG; 1.
DR PROSITE; PS00905; GTP1_OBG; 1.
DR PROSITE; PS51880; TGS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; GTP-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..399
FT /note="Developmentally-regulated G-protein 2"
FT /id="PRO_0000412609"
FT DOMAIN 63..288
FT /note="OBG-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT DOMAIN 288..366
FT /note="TGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01228"
FT REGION 372..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 69..76
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 115..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT BINDING 246..249
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01047"
FT VAR_SEQ 1..144
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_041722"
FT CONFLICT 296
FT /note="P -> R (in Ref. 6; BAD95228)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="D -> G (in Ref. 6; BAD95228)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 44803 MW; 15BFEE07552B19CE CRC64;
MGIVERIKEI EAEMARTQKN KATEYHLGQL KAKIAKLRTQ LLEPPKGSSG GGDGFEVTKY
GHGRVALIGF PSVGKSTLLT MLTGTHSEAA SYEFTTLTCI PGVIHYNDTK IQLLDLPGII
EGASEGKGRG RQVIAVAKSS DLVLMVLDAS KSEGHRQILT KELEAVGLRL NKRPPQIYFK
KKKTGGISFN TTTPLTRIDE KLCYQILHEY KIHNAEVLFR EDATVDDFID VVEGNRKYIK
CVYVYNKIDV VGIDDVDRLA RQPNSIVISC NLKLNLDRLL ARMWDEMGLV RVYSKPQSQQ
PDFDEPFVLS ADRGGCTVED FCNQVHRTLV KDMKYALVWG TSARHYPQHC GLFHHLEDED
VVQIVKKKVR EEGGRGRFKS HSNAPARIAD REKKAPLKQ
