DRGX_MOUSE
ID DRGX_MOUSE Reviewed; 263 AA.
AC Q8BYH0; A2RTS1; B2ZIF3; B2ZIF4; Q8K3F8;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Dorsal root ganglia homeobox protein;
DE AltName: Full=Dorsal root ganglion 11;
DE AltName: Full=Homeobox protein DRG11;
DE AltName: Full=Paired-related homeobox protein-like 1;
GN Name=Drgx; Synonyms=Drg11 {ECO:0000303|PubMed:15099678}, Prrxl1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RX PubMed=19598127; DOI=10.1387/ijdb.072507sr;
RA Rebelo S., Lopes C., Lima D., Reguenga C.;
RT "Expression of a Prrxl1 alternative splice variant during the development
RT of the mouse nociceptive system.";
RL Int. J. Dev. Biol. 53:1089-1095(2009).
RN [2] {ECO:0000312|EMBL:BAC30405.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC30405.1};
RC TISSUE=Spinal cord {ECO:0000312|EMBL:BAC30405.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAM49062.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-104.
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAM49062.1};
RX PubMed=15099678; DOI=10.1016/j.neuroscience.2004.02.006;
RA Vogelaar C.F., Hoekman M.F., Brakkee J.H., Bogerd J., Burbach J.P.;
RT "Developmental regulation of homeobox gene expression in dorsal root
RT ganglion neurons is not recapitulated during regeneration of the crushed
RT sciatic nerve.";
RL Neuroscience 125:645-650(2004).
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=11498051; DOI=10.1016/s0896-6273(01)00341-5;
RA Chen Z.-F., Rebelo S., White F., Malmberg A.B., Baba H., Lima D.,
RA Woolf C.J., Basbaum A.I., Anderson D.J.;
RT "The paired homeodomain protein DRG11 is required for the projection of
RT cutaneous sensory afferent fibers to the dorsal spinal cord.";
RL Neuron 31:59-73(2001).
RN [6]
RP INTERACTION WITH RGMB AND DISRUPTION PHENOTYPE.
RX PubMed=14985445; DOI=10.1523/jneurosci.4115-03.2004;
RA Samad T.A., Srinivasan A., Karchewski L.A., Jeong S.-J., Campagna J.A.,
RA Ji R.-R., Fabrizio D.A., Zhang Y., Lin H.Y., Bell E., Woolf C.J.;
RT "DRAGON: a member of the repulsive guidance molecule-related family of
RT neuronal- and muscle-expressed membrane proteins is regulated by DRG11 and
RT has neuronal adhesive properties.";
RL J. Neurosci. 24:2027-2036(2004).
CC -!- FUNCTION: Transcription factor required for the formation of correct
CC projections from nociceptive sensory neurons to the dorsal horn of the
CC spinal cord and normal perception of pain.
CC {ECO:0000269|PubMed:11498051}.
CC -!- SUBUNIT: Interacts with RGMB. {ECO:0000269|PubMed:14985445}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P54821,
CC ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-
CC ProRule:PRU00138}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BYH0-1; Sequence=Displayed;
CC Name=2; Synonyms=Prrxl1-B;
CC IsoId=Q8BYH0-2; Sequence=VSP_040392;
CC -!- TISSUE SPECIFICITY: Expressed in the dorsal horn of the spinal cord and
CC dorsal root ganglia. Isoform 1 is higher expressed than isoform 2 both
CC in the dorsal root ganglia and in the spinal cord. Isoform 2 is
CC exclusively localized in neurons primarily involved in the processing
CC of the pain somatosensory modality. {ECO:0000269|PubMed:11498051,
CC ECO:0000269|PubMed:19598127}.
CC -!- DEVELOPMENTAL STAGE: First detected at 12.5 dpc in newly generated
CC neurons adjacent to the ventricular zone of the dorsal spinal cord.
CC Later expressed in lateral region of the dorsal spinal cord and dorsal
CC root ganglia at 13.5 dpc with increased levels at 15.5 dpc.
CC {ECO:0000269|PubMed:11498051}.
CC -!- DISRUPTION PHENOTYPE: Defects in the projection pattern of nociceptive
CC sensory neurons to the dorsal horn of the developing spinal cord
CC leading to reduced sensitivity to painful stimuli. They die within 3
CC weeks of birth. Mice lacking Drgx exhibit reduced expression of Rgmb in
CC dorsal root ganglion sensory neurons. {ECO:0000269|PubMed:11498051}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000255}.
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DR EMBL; EU670677; ACD45984.1; -; mRNA.
DR EMBL; EU670678; ACD45985.1; -; mRNA.
DR EMBL; AK039633; BAC30405.1; -; mRNA.
DR EMBL; BC132613; AAI32614.1; -; mRNA.
DR EMBL; BC145917; AAI45918.1; -; mRNA.
DR EMBL; AY116506; AAM49062.1; -; mRNA.
DR CCDS; CCDS26922.1; -. [Q8BYH0-2]
DR CCDS; CCDS84110.1; -. [Q8BYH0-1]
DR RefSeq; NP_001001796.1; NM_001001796.4. [Q8BYH0-2]
DR RefSeq; NP_001334519.1; NM_001347590.1. [Q8BYH0-1]
DR RefSeq; XP_006518474.1; XM_006518411.1. [Q8BYH0-1]
DR RefSeq; XP_006518475.1; XM_006518412.3. [Q8BYH0-1]
DR RefSeq; XP_006518476.1; XM_006518413.3. [Q8BYH0-1]
DR RefSeq; XP_006518477.1; XM_006518414.3. [Q8BYH0-1]
DR RefSeq; XP_011243211.1; XM_011244909.2.
DR AlphaFoldDB; Q8BYH0; -.
DR SMR; Q8BYH0; -.
DR BioGRID; 223539; 7.
DR STRING; 10090.ENSMUSP00000064107; -.
DR iPTMnet; Q8BYH0; -.
DR PhosphoSitePlus; Q8BYH0; -.
DR MaxQB; Q8BYH0; -.
DR PaxDb; Q8BYH0; -.
DR PRIDE; Q8BYH0; -.
DR Antibodypedia; 27592; 160 antibodies from 22 providers.
DR DNASU; 107751; -.
DR Ensembl; ENSMUST00000068938; ENSMUSP00000064107; ENSMUSG00000041730. [Q8BYH0-2]
DR Ensembl; ENSMUST00000186452; ENSMUSP00000139756; ENSMUSG00000041730. [Q8BYH0-1]
DR Ensembl; ENSMUST00000187377; ENSMUSP00000140687; ENSMUSG00000041730. [Q8BYH0-2]
DR Ensembl; ENSMUST00000189022; ENSMUSP00000140337; ENSMUSG00000041730. [Q8BYH0-1]
DR GeneID; 107751; -.
DR KEGG; mmu:107751; -.
DR UCSC; uc007szf.1; mouse. [Q8BYH0-2]
DR UCSC; uc011zin.1; mouse. [Q8BYH0-1]
DR CTD; 107751; -.
DR MGI; MGI:2148204; Prrxl1.
DR VEuPathDB; HostDB:ENSMUSG00000041730; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000159008; -.
DR HOGENOM; CLU_079091_1_0_1; -.
DR InParanoid; Q8BYH0; -.
DR OMA; FGNHAPT; -.
DR OrthoDB; 1097815at2759; -.
DR PhylomeDB; Q8BYH0; -.
DR TreeFam; TF351612; -.
DR BioGRID-ORCS; 107751; 1 hit in 58 CRISPR screens.
DR ChiTaRS; Prrxl1; mouse.
DR PRO; PR:Q8BYH0; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8BYH0; protein.
DR Bgee; ENSMUSG00000041730; Expressed in trigeminal ganglion and 43 other tissues.
DR ExpressionAtlas; Q8BYH0; baseline and differential.
DR Genevisible; Q8BYH0; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; IMP:MGI.
DR GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR GO; GO:0009593; P:detection of chemical stimulus; IMP:MGI.
DR GO; GO:0016048; P:detection of temperature stimulus; IMP:MGI.
DR GO; GO:0021516; P:dorsal spinal cord development; IMP:MGI.
DR GO; GO:0007399; P:nervous system development; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR GO; GO:0001764; P:neuron migration; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050954; P:sensory perception of mechanical stimulus; IMP:MGI.
DR GO; GO:0021559; P:trigeminal nerve development; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR003654; OAR_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..263
FT /note="Dorsal root ganglia homeobox protein"
FT /id="PRO_0000248487"
FT DNA_BIND 33..92
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 88..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 204..217
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT COMPBIAS 222..250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 176..263
FT /note="GGPLCSCCVPDPMGLSFLPTYGCQSNRTASVAALRMKAREHSEAVLQSANLL
FT PSTSSSPGPASKQAPPEGSQDKTSPTKEQSEGEKSV -> DHFRAMLCSGSFGFRGEQT
FT QQRALTPNLYPFLIDERCSAVDMTQG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072, ECO:0000303|PubMed:19598127"
FT /id="VSP_040392"
SQ SEQUENCE 263 AA; 28717 MW; 412A583BA829105C CRC64;
MFYFHCPPQL EGTAPFGNHS TGDFDDGFLR RKQRRNRTTF TLQQLEALEA VFAQTHYPDV
FTREELAMKI NLTEARVQVW FQNRRAKWRK TERGASDQEP GAKEPMAEVT PPPVRNINSP
PPGDQTRSKK EALEAQQSLG RTVGPTGPFF PSCLPGTLLN TATYAQALSH VASLKGGPLC
SCCVPDPMGL SFLPTYGCQS NRTASVAALR MKAREHSEAV LQSANLLPST SSSPGPASKQ
APPEGSQDKT SPTKEQSEGE KSV
