DRL36_ARATH
ID DRL36_ARATH Reviewed; 1222 AA.
AC Q8VZC7; B9DFV7; Q9FHI5;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Probable disease resistance protein At5g45510;
GN OrderedLocusNames=At5g45510; ORFNames=MFC19.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-293, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Root;
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-293, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Probable disease resistance protein. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VZC7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VZC7-2; Sequence=VSP_041111;
CC -!- DOMAIN: The LRR repeats probably act as specificity determinant of
CC pathogen recognition. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL57696.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB09179.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AB018113; BAB09179.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95262.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95263.1; -; Genomic_DNA.
DR EMBL; AY065063; AAL57696.1; ALT_INIT; mRNA.
DR EMBL; AK316919; BAH19624.1; -; mRNA.
DR RefSeq; NP_001078719.1; NM_001085250.2. [Q8VZC7-2]
DR RefSeq; NP_199364.2; NM_123919.4. [Q8VZC7-1]
DR AlphaFoldDB; Q8VZC7; -.
DR BioGRID; 19836; 4.
DR STRING; 3702.AT5G45510.1; -.
DR iPTMnet; Q8VZC7; -.
DR PaxDb; Q8VZC7; -.
DR PRIDE; Q8VZC7; -.
DR ProteomicsDB; 224334; -. [Q8VZC7-1]
DR EnsemblPlants; AT5G45510.1; AT5G45510.1; AT5G45510. [Q8VZC7-1]
DR EnsemblPlants; AT5G45510.2; AT5G45510.2; AT5G45510. [Q8VZC7-2]
DR GeneID; 834587; -.
DR Gramene; AT5G45510.1; AT5G45510.1; AT5G45510. [Q8VZC7-1]
DR Gramene; AT5G45510.2; AT5G45510.2; AT5G45510. [Q8VZC7-2]
DR KEGG; ath:AT5G45510; -.
DR Araport; AT5G45510; -.
DR TAIR; locus:2163588; AT5G45510.
DR InParanoid; Q8VZC7; -.
DR OMA; VGNTHEE; -.
DR OrthoDB; 352436at2759; -.
DR PRO; PR:Q8VZC7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VZC7; baseline and differential.
DR Genevisible; Q8VZC7; AT.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Coiled coil; Leucine-rich repeat;
KW Nucleotide-binding; Phosphoprotein; Plant defense; Reference proteome;
KW Repeat.
FT CHAIN 1..1222
FT /note="Probable disease resistance protein At5g45510"
FT /id="PRO_0000212768"
FT REPEAT 654..676
FT /note="LRR 1"
FT REPEAT 677..699
FT /note="LRR 2"
FT REPEAT 702..724
FT /note="LRR 3"
FT REPEAT 725..747
FT /note="LRR 4"
FT REPEAT 785..806
FT /note="LRR 5"
FT REPEAT 813..835
FT /note="LRR 6"
FT REPEAT 836..856
FT /note="LRR 7"
FT REPEAT 861..883
FT /note="LRR 8"
FT REPEAT 884..906
FT /note="LRR 9"
FT REPEAT 907..929
FT /note="LRR 10"
FT REPEAT 931..951
FT /note="LRR 11"
FT REGION 171..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 263..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 122..183
FT /evidence="ECO:0000255"
FT COMPBIAS 171..199
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..325
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 49..56
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 293
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18433157,
FT ECO:0007744|PubMed:19376835"
FT VAR_SEQ 1207..1222
FT /note="DYVMVDHSDVPPPHEC -> VVCA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_041111"
FT CONFLICT 1168
FT /note="S -> G (in Ref. 4; BAH19624)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1222 AA; 137926 MW; BC500C331E95F74A CRC64;
MSDPLKMAAE TEEIKPVPAA MGEKKDVVLC KKIVETLGGG GDQRVLLVGE AGIGKTRMAQ
MVDKEASKDV LCYQTLWLHL NRKFKVNDNY IKEKKFEDEW SLYENIASQL SLYSDFEETE
VGERDEDEEE EKKVEDLLKD LKPKIEKYLL EKKEAVVKKL EDDKKKKEKE AAEKLEAEKK
LVDPAAKKAK DHGNKNPTDA AKEKTTQVVA GGEDKAQTSS ERKPYLLLIL DDEGMTSEYE
VMVHLGLEDF LKDHTPRKIL ITRRQETEEA TKSGEHAEGE ANDSQSGEKK EDTDGEDEIR
SADKEEPESQ ARVKTEEKHE KVVPPTIDDL WGSTNTYGEI TFQTTNESQD LLESFNLKEA
EALFTSSMFF KDMPNFFFDP VPGTDEKLLN HMLKKSKSLP AAINVLAKSL EYTVKSKSYK
LNKDEEERLL KEKIEMVLSA ERGNPSDQES SSESPKKASG ENPILLLAYK LFKTDGPLKD
TILDCFWHSL DFFEHCGCVY YRDLITQWIL EGYFDPVRSV EKAYQDGHSI FMELIDRGML
KIQENNVVVP EMAMRNVIDP RRGGHLGKSR LGFSRVYGGN KRKGIGKITQ LDDMIKTVQA
KKGDKITTIL VSGDRLRRVT PKKFFKNLKE LEVLGLFEPT VKPFVPSFSD QLKLLRVLII
RDCDLLKSIE ELKALTKLNT LEVSGASSLS KISEKFFESF PELRSLHLSG LKIESSPPSI
SGLKELHCLI IKDCPLLQDL PNIQELVNLE VVDVSGASGL RTCFDNADGA KKNKSKNKNF
YLLTKLQHLD FSGSQIERLP IFQDSAVAAK LHSLTRLLLR NCSKLRRLPS LKPLSGLQIL
DLSGTTSLVE MLEVCFEDKL ELKTLNLSGT NLSELATTIE DLSSLNELLL RDCINLDAIP
NIEKLENLEV IDVSGSAKLA KIEGSFEKMF YLRVVDLSGT QVETPELPAD TKIHCLKRFT
RADGKCFERD TWREIKEDIE RDRSENASSS DAVVISQEIT EKKPVEIREV ESNAPRASDC
TEKVDVNKER LLKVPIDRAL YQKALTSLVD SKIPQEEVLE INETNKLDEE ALASAEFVSF
VDCTPERVKS IFEKAKLVKG CWLRMCFDIK DPFDGVDEEN LKSLETLSIT NLLSLETISF
IAKLENLKNL SLDCCPKIKT IFPEMPASLP VLNLKHCENL EKVVVGVEVS THTNLDLKVE
NCPKFGDYVM VDHSDVPPPH EC
