DRM3_ORYSJ
ID DRM3_ORYSJ Reviewed; 680 AA.
AC Q6AUQ7; Q0DKZ6;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Probable inactive DNA (cytosine-5)-methyltransferase DRM3 {ECO:0000305};
DE AltName: Full=Protein DOMAINS REARRANGED METHYLASE 3 {ECO:0000305};
GN Name=DRM3 {ECO:0000305};
GN OrderedLocusNames=Os05g0133900 {ECO:0000312|EMBL:BAS92133.1},
GN LOC_Os05g04330 {ECO:0000305};
GN ORFNames=OSJNBa0077L08.12 {ECO:0000312|EMBL:AAT85176.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Involved in de novo DNA methylation. Involved in RNA-directed
CC DNA methylation (RdDM). {ECO:0000250|UniProtKB:Q10SU5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6AUQ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6AUQ7-2; Sequence=VSP_058617, VSP_058618;
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. DRM-methyltransferase family. {ECO:0000255|PROSITE-
CC ProRule:PRU01017}.
CC -!- CAUTION: Lacks the conserved tripeptide Ser-Pro-Cys in position 625
CC necessary for the methyltransferase activity in DRM protein (AC
CC Q9M548).
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DR EMBL; AC118288; AAT85176.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16477.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92132.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92133.1; -; Genomic_DNA.
DR EMBL; AK063482; BAG88726.1; -; mRNA.
DR RefSeq; XP_015639540.1; XM_015784054.1. [Q6AUQ7-1]
DR AlphaFoldDB; Q6AUQ7; -.
DR SMR; Q6AUQ7; -.
DR STRING; 4530.OS05T0133900-02; -.
DR REBASE; 11927; M.OsaDRM3P.
DR PaxDb; Q6AUQ7; -.
DR PRIDE; Q6AUQ7; -.
DR EnsemblPlants; Os05t0133900-01; Os05t0133900-01; Os05g0133900. [Q6AUQ7-2]
DR EnsemblPlants; Os05t0133900-02; Os05t0133900-02; Os05g0133900. [Q6AUQ7-1]
DR GeneID; 4337721; -.
DR Gramene; Os05t0133900-01; Os05t0133900-01; Os05g0133900. [Q6AUQ7-2]
DR Gramene; Os05t0133900-02; Os05t0133900-02; Os05g0133900. [Q6AUQ7-1]
DR KEGG; osa:4337721; -.
DR eggNOG; ENOG502QS8G; Eukaryota.
DR HOGENOM; CLU_006805_3_1_1; -.
DR InParanoid; Q6AUQ7; -.
DR OMA; HLAPRKE; -.
DR OrthoDB; 412711at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006306; P:DNA methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR030380; SAM_MeTfrase_DRM.
DR SUPFAM; SSF53335; SSF53335; 2.
DR PROSITE; PS51680; SAM_MT_DRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Methyltransferase; Nucleus;
KW Reference proteome; Repeat; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..680
FT /note="Probable inactive DNA (cytosine-5)-methyltransferase
FT DRM3"
FT /id="PRO_0000438154"
FT DOMAIN 45..86
FT /note="UBA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT DOMAIN 194..235
FT /note="UBA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00212"
FT DOMAIN 336..663
FT /note="SAM-dependent MTase DRM-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01017"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 665..667
FT /note="VRA -> NRG (in isoform 2)"
FT /id="VSP_058617"
FT VAR_SEQ 668..680
FT /note="Missing (in isoform 2)"
FT /id="VSP_058618"
SQ SEQUENCE 680 AA; 76131 MW; B4B63A35302974D7 CRC64;
MVKVEDDVEG SGINASVGDL RDAAVNPQPA LLRATVKEEE GQPSSSSSHV RSQFIGMGFS
PMLVDRVLQK HGDRDSDTIL EALLSQSALQ KSGSESGSLG DLFDSDNEEN SSHFAPRKEV
IQDIKVEADS SSEKRSYLLS TMNFSQREVD LALNQLGEEA SLEQLVDFIV TGQVSGCSGG
NENGDASNEV KDESLFGVMD KTLHLLQMGF TEEEVSSVID KAGPEATVLE LADTIFARRI
ASSIEQKEVK VEPDFLDETE TSYSAYHPSN SGLRYYDDDH DNIRIKRAKH MFIDDSAGSS
SRAGNQPNLD PWLKDHRATT SDGSVKEEFD AMTPGIRRNV RSDVANPPYF LYGNVVEIPK
ATWRQLSEFL YNVEPEFVNS QFFSALSRKE GYIHNLPTEG RRNLVPRSPM TIEEAFPFTR
QCWPSWDTRK QLNSVATEVA GIEQLCERLG KMVRDSGGYL SQEKKTHIMH QCKLANLIWV
GPDRLSPLDP QQVERILGYP RKHTNLFGLN PQDRIEAMRY SFQTDTLGYL LSVLKDLYPD
GLRVLSIYSG IGGAAIALHR LGIPLQCVVS VDQSDTNRKI LRRWWSNTEQ KGQLRQINTI
WKLKINVLED LVKEFGGFDI IIGGNFSSCK GGTTVNSSMG MDSNQFFEYV RVVQRVKHIM
GRLQVRAHES ARHRHRSPSN
