ID   DRMA_BACP9              Reviewed;        1119 AA.
AC   P0DW05;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=DISARM protein DrmA {ECO:0000303|PubMed:29085076};
DE   AltName: Full=Probable ATP-dependent helicase DrmA {ECO:0000305};
GN   Name=drmA {ECO:0000303|PubMed:29085076};
GN   OrderedLocusNames=BaLi_c08320 {ECO:0000312|EMBL:AGN35227.1};
OS   Bacillus paralicheniformis (strain ATCC 9945a / NCIMB 11709 / CD-2).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=766760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=23908277; DOI=10.1128/genomea.00525-13;
RA   Rachinger M., Volland S., Meinhardt F., Daniel R., Liesegang H.;
RT   "First Insights into the Completely Annotated Genome Sequence of Bacillus
RT   licheniformis Strain 9945A.";
RL   Genome Announc. 1:0-0(2013).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND EXPRESSION IN B.SUBTILIS.
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=29085076; DOI=10.1038/s41564-017-0051-0;
RA   Ofir G., Melamed S., Sberro H., Mukamel Z., Silverman S., Yaakov G.,
RA   Doron S., Sorek R.;
RT   "DISARM is a widespread bacterial defence system with broad anti-phage
RT   activities.";
RL   Nat. Microbiol. 3:90-98(2018).
CC   -!- FUNCTION: Component of antiviral defense system DISARM (defense island
CC       system associated with restriction-modification), composed of DrmE,
CC       DrmA, DrmB, DrmC and DrmMII. DISARM is probably a multi-gene
CC       restriction module, this subunit is probably a helicase. Expression of
CC       DISARM in B.subtilis (strain BEST7003) confers resistance to phages Nf,
CC       phi29, phi105, phi3T, SPO1, SPR and SPP1. Protection is over 10(7)-fold
CC       against phi3T, 10(4)-10(5)-fold against Nf, phi29, phi105 and SPR, 100-
CC       fold against SPO1 and 10-fold against SPP1. DISARM does not interfere
CC       with phage adsorption, but instead interferes with (phi3T) DNA
CC       replication early in its cycle, preventing replication, circularization
CC       and lysogeny and probably causes phage DNA degradation (DNA is degraded
CC       in SPP1-infected cells). {ECO:0000269|PubMed:29085076}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the DISARM system does
CC       not confer Nf, phi3T or SPO1 resistance in B.subtilis.
CC       {ECO:0000269|PubMed:29085076}.
CC   -!- SIMILARITY: Belongs to the helicase family. {ECO:0000305}.
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DR   EMBL; CP005965; AGN35227.1; -; Genomic_DNA.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; ATP-binding; Cytoplasm; Helicase; Hydrolase;
KW   Nucleotide-binding; Restriction system.
FT   CHAIN           1..1119
FT                   /note="DISARM protein DrmA"
FT                   /id="PRO_0000456311"
FT   DOMAIN          813..986
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
SQ   SEQUENCE   1119 AA;  129345 MW;  C3DEDBBEF50CCC10 CRC64;
     MSKAEREMYK KVRRIMVSDL KRDLIGPAHD ESDVIYEAPS QAYITGILYP LESEVETEKS
     LEDVQFADVY DEPESGMEED VEQQRNSELE QEAEEEKITP NKKFKHQNSL GIRCYVRETT
     DHIRASIAWG RYTSSKKFDS ERKREVILWT RQIECFEEYI SLSSFDQNID IPITQEVSLV
     VSKKKLSGTD VFLVSVFLVN KRVDKNSNNA MFQCELTLTH EEGKGIFLCE NEARKDKNDF
     SEFLYRNKPV FAKGFGCAVT WKAESSNYAS ELKSAFIPEH EVESMSTDLP YDENYGELPK
     GYFSIKQFAE ADDQRETIDK LNNLANRYES WINKLPVDKV ANKEATERVI KDCQATLTRI
     RRGIQLLANE ENNKAFVAFK FMNTVMHTQD AMKRYSRNNK TTTLETEIRK ENLEWRPFQL
     AFILLNLEGL VDLKSKDRKI VDLLWFPTGG GKTEAYLGIC AFLLGYRRLF ASTSSAYERD
     GGVTILLRYT LRLLTTQQRD RLMRMISACE YTRQKTNAFG QSEFSVGFWV GAQVTVNKLD
     DLTENEYYLD RDKVLKEYEK IEKQVIECPC CGTKGLEYKF LPSRDTKTKK TGIKIFCTNE
     SCFFSKTHIP VYLVDEEIYR KLPTVVISTV DKFARLPWDE KTAALFGKVN RFCERCGYIA
     EGEKHEKSHR NPKASVHDVK PFYPPELIIQ DELHLITGPL GTVYGGYETV IEELSTAHEG
     EDYLKPKYIA ATATIKNAEV QVEKVFGRKL TQQFPPPGLK VEDSFFARER NLDEFPFRLY
     AGVCVSGHSM KTVLLRIYAV LLQTTEHLLE DEELSKYIDP YRTLVGYFNS IRELGGAVRL
     LEDDIKKRIQ TLQKKYKYSK QRYISRKPEL TSRVPSSRIP KVLEQLEKEI GNEELDVVLA
     TNMISVGMDV DRLGLMVITG QPKQTSEYIQ SSSRVGRSKP GLVITVYNPY RPRDMSHYQN
     FKGYHQRLYH FVEGTTATPY ASRARDRFLH AIAVALLRLS YPELAKNLDA KNIKDMDLNY
     LKDVVKKRVS TVEYRNVSDT MEHLQHFLDE WISRAVSEDN LQYYFNPKTR SARIGNQSRL
     LARFSEEKKH GGKPTLDSMR QVEGTSSLYI YEGWNSSEE