ID   DRMC_BACP9              Reviewed;         240 AA.
AC   P0DW07;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=DISARM protein DrmC {ECO:0000303|PubMed:29085076};
DE            EC=3.1.-.- {ECO:0000305};
GN   Name=drmC {ECO:0000303|PubMed:29085076};
GN   OrderedLocusNames=BaLi_c08340 {ECO:0000312|EMBL:AGN35229.1};
OS   Bacillus paralicheniformis (strain ATCC 9945a / NCIMB 11709 / CD-2).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=766760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=23908277; DOI=10.1128/genomea.00525-13;
RA   Rachinger M., Volland S., Meinhardt F., Daniel R., Liesegang H.;
RT   "First Insights into the Completely Annotated Genome Sequence of Bacillus
RT   licheniformis Strain 9945A.";
RL   Genome Announc. 1:0-0(2013).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND EXPRESSION IN B.SUBTILIS.
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=29085076; DOI=10.1038/s41564-017-0051-0;
RA   Ofir G., Melamed S., Sberro H., Mukamel Z., Silverman S., Yaakov G.,
RA   Doron S., Sorek R.;
RT   "DISARM is a widespread bacterial defence system with broad anti-phage
RT   activities.";
RL   Nat. Microbiol. 3:90-98(2018).
CC   -!- FUNCTION: Component of antiviral defense system DISARM (defense island
CC       system associated with restriction-modification), composed of DrmE,
CC       DrmA, DrmB, DrmC and DrmMII. DISARM is probably a multi-gene
CC       restriction module, this subunit is probably a phospholipase or
CC       nuclease. Expression of DISARM in B.subtilis (strain BEST7003) confers
CC       resistance to phages Nf, phi29, phi105, phi3T, SPO1, SPR and SPP1.
CC       Protection is over 10(7)-fold against phi3T, 10(4)-10(5)-fold against
CC       Nf, phi29, phi105 and SPR, 100-fold against SPO1 and 10-fold against
CC       SPP1. DISARM does not interfere with phage adsorption, but instead
CC       interferes with (phi3T) DNA replication early in its cycle, preventing
CC       replication, circularization and lysogeny and probably causes phage DNA
CC       degradation (DNA is degraded in SPP1-infected cells).
CC       {ECO:0000269|PubMed:29085076}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Differentially required for bacteriophage
CC       defense; when this gene is missing the DISARM system still confers
CC       resistance against phi105, phi29, phi3T, SPP1 and SPR but reduced
CC       protection against Nf and SPO1 in B.subtilis.
CC       {ECO:0000269|PubMed:29085076}.
CC   -!- SIMILARITY: Belongs to the phospholipase D family. {ECO:0000305}.
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DR   EMBL; CP005965; AGN35229.1; -; Genomic_DNA.
DR   PROSITE; PS50035; PLD; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Cytoplasm; Hydrolase; Restriction system.
FT   CHAIN           1..240
FT                   /note="DISARM protein DrmC"
FT                   /id="PRO_0000456312"
FT   DOMAIN          174..201
FT                   /note="PLD phosphodiesterase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   ACT_SITE        179
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   ACT_SITE        181
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   ACT_SITE        186
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
SQ   SEQUENCE   240 AA;  27389 MW;  659EBAD1E0F678D2 CRC64;
     MRNVTRSHMV LHQIQSVLKK YEEVSAQELY ESLDKGTVQR EFGVRSVDLS RLSVSEEQFS
     ELLLLFKLYS DQQQQSSIEF VATVPSEVDV RLRKTIAVIR EMIHGAQNTI LVTGYAVSEY
     VDEIMERVLE KALAGVNVDI FLDRNPQTDR YIENIRGRNL PSNFNVYVYK GSQGYSSLHA
     KVIMVDEEKA FVSSANLSYN GIVNNIEIGT LVGGEKILVI KNVLLELVKN NYFEKIIWYA