ID   DRME_BACP9              Reviewed;         799 AA.
AC   P0DW04;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=DISARM protein DrmE {ECO:0000303|PubMed:29085076};
GN   Name=drmE {ECO:0000303|PubMed:29085076};
GN   OrderedLocusNames=BaLi_c08310 {ECO:0000312|EMBL:AGN35226.1};
OS   Bacillus paralicheniformis (strain ATCC 9945a / NCIMB 11709 / CD-2).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=766760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=23908277; DOI=10.1128/genomea.00525-13;
RA   Rachinger M., Volland S., Meinhardt F., Daniel R., Liesegang H.;
RT   "First Insights into the Completely Annotated Genome Sequence of Bacillus
RT   licheniformis Strain 9945A.";
RL   Genome Announc. 1:0-0(2013).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND EXPRESSION IN B.SUBTILIS.
RC   STRAIN=ATCC 9945a / NCIMB 11709 / CD-2;
RX   PubMed=29085076; DOI=10.1038/s41564-017-0051-0;
RA   Ofir G., Melamed S., Sberro H., Mukamel Z., Silverman S., Yaakov G.,
RA   Doron S., Sorek R.;
RT   "DISARM is a widespread bacterial defence system with broad anti-phage
RT   activities.";
RL   Nat. Microbiol. 3:90-98(2018).
CC   -!- FUNCTION: Component of antiviral defense system DISARM (defense island
CC       system associated with restriction-modification), composed of DrmE,
CC       DrmA, DrmB, DrmC and DrmMII. DISARM is probably a multi-gene
CC       restriction module, this subunit has an unknown function. Expression of
CC       DISARM in B.subtilis (strain BEST7003) confers resistance to phages Nf,
CC       phi29, phi105, phi3T, SPO1, SPR and SPP1. Protection is over 10(7)-fold
CC       against phi3T, 10(4)-10(5)-fold against Nf, phi29, phi105 and SPR, 100-
CC       fold against SPO1 and 10-fold against SPP1. DISARM does not interfere
CC       with phage adsorption, but instead interferes with (phi3T) DNA
CC       replication early in its cycle, preventing replication, circularization
CC       and lysogeny and probably causes phage DNA degradation (DNA is degraded
CC       in SPP1-infected cells). {ECO:0000269|PubMed:29085076}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the DISARM system does
CC       not confer Nf, phi3T or SPO1 resistance in B.subtilis.
CC       {ECO:0000269|PubMed:29085076}.
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DR   EMBL; CP005965; AGN35226.1; -; Genomic_DNA.
PE   4: Predicted;
KW   Antiviral defense; Cytoplasm; Restriction system.
FT   CHAIN           1..799
FT                   /note="DISARM protein DrmE"
FT                   /id="PRO_0000456310"
SQ   SEQUENCE   799 AA;  93172 MW;  8D95AAC7EBF8BE36 CRC64;
     MLYSTSDKSL VHCSVPQQIF YRAEECMGDE IVGSLLCKEN ILLCTPRKYI FPHLVLRALN
     MDETMGFQKR ENPSKRAEIL VVTNRLEMLA FLETCRVNAE RLFQICSNIH QYIGYCNMGD
     TYFARVYWRH ILYHYYEGKL PQDVPLHYIY PVASGYRKFN ALSRGNRNIL GRKDSQNPTI
     YVTENLDMLN EQEHSFDYIF VDCSYIKKGL SNLPKSTLLF FDNLLDDRIP YLQKSAVKNY
     IVDGDCIQNI DENEIQQPMI NIEELLHKVS IHSLDVEYVK SSFEQEIERL IHLLDKLRKG
     KFSRYDTNVA AKLVYILIRL PIGAGLYDLI ASMQPYWDTV LGLLQELKDS ESRYENDSFE
     EMVSLFEDIL YKHSLDRSNP KGDELKAFIL NEVKQGKSVC VVSNSRKNQL ALKEFISLAM
     GMTIEELAEY DLQFFVSKDI WAQDITVHCD SLVMYSAINF RDLQSLLKIS YKRAKLYLYS
     SEINLITQKL KTILEAENYA LRHFVQNSTQ QDSTNFYRYL YNRFNKFARQ KVIGLNSAAA
     DLLEKSTTVS PAVYRGEKDY KGTDAVKATL VHFTDGSVSF FTKNSAVYVL DNKNKRVTHK
     HFHEIGLKDT ILFVDNDARK DLYRIFIQSV DAKDTSKQAY LSIKKWRELY EEKFMEKRMD
     DDRLFRLMRT AGWNKTTKSV LRNWRTGYSY GPRDREDIMV LGEVLGINTF VEDVQTYYNA
     MSKIRVERRK ASRILNKLIY SSKQVLGNED SAILARYNLT LEQLNESLVT KSIKEIVSDR
     MYYIKPAEVG LLYNVDAKE