DRM_DROME
ID DRM_DROME Reviewed; 81 AA.
AC Q9VQS6; A4V041; Q49UC1; Q8SZH7;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Protein drumstick;
GN Name=drm {ECO:0000312|EMBL:AAF51088.2}; ORFNames=CG10016;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF51088.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF51088.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP SEQUENCE REVISION.
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11784087; DOI=10.1006/dbio.2001.0483;
RA Iwaki D.D., Johansen K.A., Singer J.B., Lengyel J.A.;
RT "Drumstick, bowl, and lines are required for patterning and cell
RT rearrangement in the Drosophila embryonic hindgut.";
RL Dev. Biol. 240:611-626(2001).
RN [6] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH LIN, AND MUTAGENESIS OF
RP GLU-25; GLU-45; ARG-46 AND CYS-57.
RX PubMed=12117814; DOI=10.1242/dev.129.15.3645;
RA Green R.B., Hatini V., Johansen K.A., Liu X.-J., Lengyel J.A.;
RT "Drumstick is a zinc finger protein that antagonizes Lines to control
RT patterning and morphogenesis of the Drosophila hindgut.";
RL Development 129:3645-3656(2002).
RN [7] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14597202; DOI=10.1016/j.ydbio.2003.07.011;
RA Hao I., Green R.B., Dunaevsky O., Lengyel J.A., Rauskolb C.;
RT "The odd-skipped family of zinc finger genes promotes Drosophila leg
RT segmentation.";
RL Dev. Biol. 263:282-295(2003).
RN [8] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14568103; DOI=10.1016/j.mod.2003.08.001;
RA Johansen K.A., Green R.B., Iwaki D.D., Hernandez J.B., Lengyel J.A.;
RT "The Drm-Bowl-Lin relief-of-repression hierarchy controls fore- and hindgut
RT patterning and morphogenesis.";
RL Mech. Dev. 120:1139-1151(2003).
CC -!- FUNCTION: Putative transcription factor. May function redundantly with
CC odd and sob in leg joint formation during the larval stages, acting
CC downstream of Notch activation. Acts in a hierarchy during foregut and
CC hindgut patterning and morphogenesis, antagonizing lin to relieve the
CC repressive effect on bowl. Involved in cell rearrangement during
CC elongation of the embryonic hindgut. Regulates expression of hindgut
CC patterning genes to establish the small intestine region of the
CC embryonic hindgut. Required in the foregut for spatially localized gene
CC expression and morphogenesis of the proventriculus.
CC {ECO:0000269|PubMed:11784087, ECO:0000269|PubMed:12117814,
CC ECO:0000269|PubMed:14568103, ECO:0000269|PubMed:14597202}.
CC -!- SUBUNIT: Interacts with lin. {ECO:0000269|PubMed:12117814}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Has two temporally distinct modes of expression
CC during early embryogenesis. Expressed in seven stripes at the
CC blastoderm stage, then during gastrulation the seven primary stripes
CC are supplemented by seven secondary stripes which appear in alternate
CC segments. This results in the labelling of each of the 14 segments in
CC the extended germ band. Also expressed in the anterior gut primordium
CC starting at stage 5 and in the embryonic foregut (including the
CC proventriculus) and hindgut. Strongly expressed in a segmentally
CC repeated pattern in the leg disk at the distal edge of each presumptive
CC leg segment except in tarsal segments 1 to 4. In early third instar leg
CC disks, expression starts in a proximal ring corresponding to the
CC presumptive coxa. The number of segmental rings progressively increases
CC to 5 by the end of larval development. {ECO:0000269|PubMed:11784087,
CC ECO:0000269|PubMed:12117814, ECO:0000269|PubMed:14568103,
CC ECO:0000269|PubMed:14597202}.
CC -!- DOMAIN: The C2H2-type domain 1 is essential for binding to and
CC repressing lin, while the C2H2-type domain 2 contributes to lin
CC binding.
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DR EMBL; AE014134; AAF51088.2; -; Genomic_DNA.
DR EMBL; AE014134; AAN10375.1; -; Genomic_DNA.
DR EMBL; AY070883; AAZ41785.1; -; mRNA.
DR RefSeq; NP_536788.2; NM_080527.3.
DR RefSeq; NP_722921.1; NM_164545.2.
DR AlphaFoldDB; Q9VQS6; -.
DR SMR; Q9VQS6; -.
DR BioGRID; 72396; 67.
DR IntAct; Q9VQS6; 63.
DR STRING; 7227.FBpp0077219; -.
DR PaxDb; Q9VQS6; -.
DR PRIDE; Q9VQS6; -.
DR DNASU; 49638; -.
DR EnsemblMetazoa; FBtr0077530; FBpp0077219; FBgn0024244.
DR EnsemblMetazoa; FBtr0077531; FBpp0077220; FBgn0024244.
DR GeneID; 49638; -.
DR KEGG; dme:Dmel_CG10016; -.
DR UCSC; CG10016-RA; d. melanogaster.
DR CTD; 49638; -.
DR FlyBase; FBgn0024244; drm.
DR VEuPathDB; VectorBase:FBgn0024244; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000172968; -.
DR HOGENOM; CLU_2576967_0_0_1; -.
DR InParanoid; Q9VQS6; -.
DR OMA; MRAKCEF; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9VQS6; -.
DR BioGRID-ORCS; 49638; 0 hits in 1 CRISPR screen.
DR ChiTaRS; drm; fly.
DR GenomeRNAi; 49638; -.
DR PRO; PR:Q9VQS6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0024244; Expressed in proventriculus primordium (Drosophila) and 71 other tissues.
DR ExpressionAtlas; Q9VQS6; baseline and differential.
DR Genevisible; Q9VQS6; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048565; P:digestive tract development; IDA:FlyBase.
DR GO; GO:0048617; P:embryonic foregut morphogenesis; IMP:UniProtKB.
DR GO; GO:0048619; P:embryonic hindgut morphogenesis; IMP:UniProtKB.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:UniProtKB.
DR GO; GO:0007440; P:foregut morphogenesis; IMP:FlyBase.
DR GO; GO:0007442; P:hindgut morphogenesis; IMP:FlyBase.
DR GO; GO:0016348; P:imaginal disc-derived leg joint morphogenesis; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Pair-rule protein; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..81
FT /note="Protein drumstick"
FT /id="PRO_0000046920"
FT ZN_FING 26..48
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 55..75
FT /note="C2H2-type 2; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT MUTAGEN 25
FT /note="E->K: In drm5; foregut proventriculus
FT morphologically and functionally defective."
FT /evidence="ECO:0000269|PubMed:12117814"
FT MUTAGEN 45
FT /note="E->K: In drm4; foregut proventriculus
FT morphologically and functionally defective."
FT /evidence="ECO:0000269|PubMed:12117814"
FT MUTAGEN 46
FT /note="R->C: In drm6; foregut proventriculus and hindgut
FT morphologically and functionally defective. Abolishes lin
FT binding. Abolishes lin-inhibiting activity."
FT /evidence="ECO:0000269|PubMed:12117814"
FT MUTAGEN 46
FT /note="R->H: In drm2; foregut proventriculus
FT morphologically and functionally defective."
FT /evidence="ECO:0000269|PubMed:12117814"
FT MUTAGEN 57
FT /note="C->G: Reduces lin binding. No effect on lin-
FT inhibiting activity."
FT /evidence="ECO:0000269|PubMed:12117814"
SQ SEQUENCE 81 AA; 10120 MW; D4324010DA5FD394 CRC64;
MFAVMRIDND DCRSDFRRKM RPKCEFICKY CQRRFTKPYN LMIHERTHKS PEITYSCEVC
GKYFKQRDNL RQHRCSQCVW R
