DRP5A_ARATH
ID DRP5A_ARATH Reviewed; 817 AA.
AC F4HPR5; Q9LNN8;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Dynamin-related protein 5A;
DE AltName: Full=Protein ARC5-like;
GN Name=DRP5A; OrderedLocusNames=At1g53140; ORFNames=F8L10.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14750516; DOI=10.1023/b:plan.0000007000.29697.81;
RA Hong Z., Bednarek S.Y., Blumwald E., Hwang I., Jurgens G., Menzel D.,
RA Osteryoung K.W., Raikhel N.V., Shinozaki K., Tsutsumi N., Verma D.P.S.;
RT "A unified nomenclature for Arabidopsis dynamin-related large GTPases based
RT on homology and possible functions.";
RL Plant Mol. Biol. 53:261-265(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=18809930; DOI=10.1073/pnas.0802412105;
RA Miyagishima S.Y., Kuwayama H., Urushihara H., Nakanishi H.;
RT "Evolutionary linkage between eukaryotic cytokinesis and chloroplast
RT division by dynamin proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:15202-15207(2008).
CC -!- FUNCTION: Probable microtubule-associated force-producing protein that
CC is targeted to the forming cell plate during cytokinesis. May play a
CC role in cell division. {ECO:0000269|PubMed:18809930}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18809930}.
CC Cytoplasm, cytoskeleton {ECO:0000269|PubMed:18809930}. Cytoplasm,
CC cytoskeleton, phragmoplast {ECO:0000269|PubMed:18809930}.
CC Note=Localizes in the forming cell plate during cytokinesis.
CC -!- TISSUE SPECIFICITY: Expressed in root and leaf meristems.
CC {ECO:0000269|PubMed:18809930}.
CC -!- INDUCTION: Expression is cell-cycle regulated. Expressed during mitosis
CC from the prophase to the telophase. {ECO:0000269|PubMed:18809930}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but when grown at 16 degrees Celsius, seedlings grow slowly
CC and root tip cells form incomplete or twisted cell plates.
CC {ECO:0000269|PubMed:18809930}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. Dynamin/Fzo/YdjA family. {ECO:0000255|PROSITE-
CC ProRule:PRU01055}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF87857.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX842486; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC022520; AAF87857.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32895.1; -; Genomic_DNA.
DR EMBL; BX842486; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A96572; A96572.
DR RefSeq; NP_175722.1; NM_104193.5.
DR AlphaFoldDB; F4HPR5; -.
DR SMR; F4HPR5; -.
DR STRING; 3702.AT1G53140.1; -.
DR iPTMnet; F4HPR5; -.
DR PaxDb; F4HPR5; -.
DR PRIDE; F4HPR5; -.
DR ProteomicsDB; 224370; -.
DR EnsemblPlants; AT1G53140.1; AT1G53140.1; AT1G53140.
DR GeneID; 841748; -.
DR Gramene; AT1G53140.1; AT1G53140.1; AT1G53140.
DR KEGG; ath:AT1G53140; -.
DR Araport; AT1G53140; -.
DR TAIR; locus:2037026; AT1G53140.
DR eggNOG; KOG0446; Eukaryota.
DR HOGENOM; CLU_020194_0_0_1; -.
DR InParanoid; F4HPR5; -.
DR OMA; ICYENDF; -.
DR OrthoDB; 178977at2759; -.
DR PRO; PR:F4HPR5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HPR5; baseline and differential.
DR Genevisible; F4HPR5; AT.
DR GO; GO:0009504; C:cell plate; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0000911; P:cytokinesis by cell plate formation; IMP:TAIR.
DR CDD; cd08771; DLP_1; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR022812; Dynamin.
DR InterPro; IPR001401; Dynamin_GTPase.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR030381; G_DYNAMIN_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11566; PTHR11566; 1.
DR Pfam; PF00350; Dynamin_N; 1.
DR PRINTS; PR00195; DYNAMIN.
DR SMART; SM00053; DYNc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51718; G_DYNAMIN_2; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; GTP-binding; Hydrolase;
KW Microtubule; Motor protein; Nucleotide-binding; Reference proteome.
FT CHAIN 1..817
FT /note="Dynamin-related protein 5A"
FT /id="PRO_0000415908"
FT DOMAIN 59..346
FT /note="Dynamin-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..76
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 95..97
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 175..178
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 244..247
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 280..283
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 405..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 616..658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 632..646
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 69..76
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 175..179
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 244..247
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 817 AA; 90616 MW; 2BB0058AE97A5EF1 CRC64;
MANSNTYLTT PTKTPSSRRN QQSQSKMQSH SKDPINAESR SRFEAYNRLQ AAAVAFGEKL
PIPEIVAIGG QSDGKSSLLE ALLGFRFNVR EVEMGTRRPL ILQMVHDLSA LEPRCRFQDE
DSEEYGSPIV SATAVADVIR SRTEALLKKT KTAVSPKPIV MRAEYAHCPN LTIIDTPGFV
LKAKKGEPET TPDEILSMVK SLASPPHRIL LFLQQSSVEW CSSLWLDAVR EIDSSFRRTI
VVVSKFDNRL KEFSDRGEVD RYLSASGYLG ENTRPYFVAL PKDRSTISND EFRRQISQVD
TEVIRHLREG VKGGFDEEKF RSCIGFGSLR DFLESELQKR YKEAAPATLA LLEERCSEVT
DDMLRMDMKI QATSDVAHLR KAAMLYTASI SNHVGALIDG AANPAPEQWG KTTEEERGES
GIGSWPGVSV DIKPPNAVLK LYGGAAFERV IHEFRCAAYS IECPPVSREK VANILLAHAG
RGGGRGVTEA SAEIARTAAR SWLAPLLDTA CDRLAFVLGS LFEIALERNL NQNSEYEKKT
ENMDGYVGFH AAVRNCYSRF VKNLAKQCKQ LVRHHLDSVT SPYSMACYEN NYHQGGAFGA
YNKFNQASPN SFCFELSDTS RDEPMKDQEN IPPEKNNGQE TTPGKGGESH ITVPETPSPD
QPCEIVYGLV KKEIGNGPDG VGARKRMARM VGNRNIEPFR VQNGGLMFAN ADNGMKSSSA
YSEICSSAAQ HFARIREVLV ERSVTSTLNS GFLTPCRDRL VVALGLDLFA VNDDKFMDMF
VAPGAIVVLQ NERQQLQKRQ KILQSCLTEF KTVARSL
