DRRB_MYCTU
ID DRRB_MYCTU Reviewed; 289 AA.
AC P9WG23; L0TB30; P96206; Q7D6E7;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=Doxorubicin resistance ABC transporter permease protein DrrB;
GN Name=drrB; OrderedLocusNames=Rv2937;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION IN DOXORUBICIN RESISTANCE, AND SUBCELLULAR LOCATION.
RX PubMed=12057006; DOI=10.1042/bj20020615;
RA Choudhuri B.S., Bhakta S., Barik R., Basu J., Kundu M., Chakrabarti P.;
RT "Overexpression and functional characterization of an ABC (ATP-binding
RT cassette) transporter encoded by the genes drrA and drrB of Mycobacterium
RT tuberculosis.";
RL Biochem. J. 367:279-285(2002).
RN [3]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Part of the ABC transporter complex DrrABC involved in
CC doxorubicin resistance. Probably responsible for the translocation of
CC the substrate across the membrane. {ECO:0000269|PubMed:12057006}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (DrrA) and
CC two transmembrane proteins (DrrB and DrrC). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:12057006};
CC Multi-pass membrane protein {ECO:0000305|PubMed:12057006}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC -!- SIMILARITY: Belongs to the ABC-2 integral membrane protein family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45740.1; -; Genomic_DNA.
DR PIR; E70984; E70984.
DR RefSeq; NP_217453.1; NC_000962.3.
DR RefSeq; WP_003414848.1; NZ_NVQJ01000015.1.
DR AlphaFoldDB; P9WG23; -.
DR STRING; 83332.Rv2937; -.
DR PaxDb; P9WG23; -.
DR DNASU; 887968; -.
DR GeneID; 45426925; -.
DR GeneID; 887968; -.
DR KEGG; mtu:Rv2937; -.
DR TubercuList; Rv2937; -.
DR eggNOG; COG0842; Bacteria.
DR OMA; EATAPMM; -.
DR PhylomeDB; P9WG23; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IDA:MTBBASE.
DR GO; GO:0043215; P:daunorubicin transport; IEA:InterPro.
DR GO; GO:0071770; P:DIM/DIP cell wall layer assembly; IMP:MTBBASE.
DR GO; GO:1900753; P:doxorubicin transport; IEA:InterPro.
DR GO; GO:0008610; P:lipid biosynthetic process; IMP:MTBBASE.
DR GO; GO:0046677; P:response to antibiotic; IDA:UniProtKB.
DR GO; GO:0055085; P:transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR000412; ABC_2_transport.
DR InterPro; IPR004377; ABC_transpt_DrrB/DrrC.
DR PIRSF; PIRSF006648; DrrB; 1.
DR TIGRFAMs; TIGR00025; Mtu_efflux; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..289
FT /note="Doxorubicin resistance ABC transporter permease
FT protein DrrB"
FT /id="PRO_0000393226"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 47..282
FT /note="ABC transmembrane type-2"
SQ SEQUENCE 289 AA; 31109 MW; 45ED403F53C54A34 CRC64;
MSGPAIDASP ALTFNQSSAS IQQRRLSTGR QMWVLYRRFA APSLLNGEVL TTVGAPIIFM
VGFYIPFAIP WNQFVGGASS GVASNLGQYI TPLVTLQAVS FAAIGSGFRA ATDSLLGVNR
RFQSMPMAPL TPLLARVWVA VDRCFTGLVI SLVCGYVIGF RFHRGALYIV GFCLLVIAIG
AVLSFAADLV GTVTRNPDAM LPLLSLPILI FGLLSIGLMP LKLFPHWIHP FVRNQPISQF
VAALRALAGD TTKTASQVSW PVMAPTLTWL FAFVVILALS STIVLARRP
