ID   DRS1_AGALE              Reviewed;          32 AA.
AC   P0DTD6;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Dermaseptin-L1 {ECO:0000303|PubMed:17561225};
DE            Short=DRS-L1 {ECO:0000303|PubMed:17561225};
OS   Agalychnis lemur (Lemur leaf frog) (Hylomantis lemur).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Agalychnis.
OX   NCBI_TaxID=317382;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=17561225; DOI=10.1016/j.toxicon.2007.04.017;
RA   Conlon J.M., Woodhams D.C., Raza H., Coquet L., Leprince J., Jouenne T.,
RA   Vaudry H., Rollins-Smith L.A.;
RT   "Peptides with differential cytolytic activity from skin secretions of the
RT   lemur leaf frog Hylomantis lemur (Hylidae: Phyllomedusinae).";
RL   Toxicon 50:498-506(2007).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18644413; DOI=10.1016/j.peptides.2008.06.017;
RA   Amiche M., Ladram A., Nicolas P.;
RT   "A consistent nomenclature of antimicrobial peptides isolated from frogs of
RT   the subfamily Phyllomedusinae.";
RL   Peptides 29:2074-2082(2008).
CC   -!- FUNCTION: Antimicrobial peptide active against the Gram-negative
CC       bacterium E.coli (MIC=8 uM) but inactive against the Gram-positive
CC       bacterium S.aureus (PubMed:17561225). Also inhibits growth of zoospores
CC       of the chytrid fungus B.dendrobatidis at high concentrations (above 25
CC       uM) (PubMed:17561225). Shows anticancer activities since it is
CC       cytolytic against HepG2 human hepatoma-derived cells (LC(50)=45 uM)
CC       (PubMed:17561225). Is only weakly hemolytic on human erythrocytes
CC       (PubMed:17561225). {ECO:0000269|PubMed:17561225}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17561225}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:17561225}.
CC   -!- MASS SPECTROMETRY: Mass=3192.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17561225};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Dermaseptin subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=0964";
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DR   AlphaFoldDB; P0DTD6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR022731; Dermaseptin.
DR   Pfam; PF12121; DD_K; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Secreted.
FT   PEPTIDE         1..32
FT                   /note="Dermaseptin-L1"
FT                   /id="PRO_0000449602"
SQ   SEQUENCE   32 AA;  3195 MW;  256238076DAE4FBE CRC64;
     GLWSKIKEAA KAAGKAALNA VTGLVNQGDQ PS