DRS1_CRYNB
ID DRS1_CRYNB Reviewed; 808 AA.
AC P0CQ93; Q55TT3; Q5KIK3;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=ATP-dependent RNA helicase DRS1;
DE EC=3.6.4.13;
GN Name=DRS1; OrderedLocusNames=CNBD3700;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: ATP-binding RNA helicase involved in ribosome assembly.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: Associates with pre-ribosomal particles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX27/DRS1
CC subfamily. {ECO:0000305}.
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DR EMBL; AAEY01000020; EAL21316.1; -; Genomic_DNA.
DR RefSeq; XP_775963.1; XM_770870.1.
DR AlphaFoldDB; P0CQ93; -.
DR SMR; P0CQ93; -.
DR EnsemblFungi; AAW43165; AAW43165; CND02650.
DR EnsemblFungi; EAL21316; EAL21316; CNBD3700.
DR GeneID; 4935759; -.
DR KEGG; cnb:CNBD3700; -.
DR VEuPathDB; FungiDB:CNBD3700; -.
DR HOGENOM; CLU_003041_3_2_1; -.
DR Proteomes; UP000001435; Chromosome 4.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IEA:EnsemblFungi.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IEA:EnsemblFungi.
DR GO; GO:0006364; P:rRNA processing; IEA:EnsemblFungi.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Ribosome biogenesis; RNA-binding.
FT CHAIN 1..808
FT /note="ATP-dependent RNA helicase DRS1"
FT /id="PRO_0000410257"
FT DOMAIN 253..431
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 442..621
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 27..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 660..808
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 575..645
FT /evidence="ECO:0000255"
FT MOTIF 222..250
FT /note="Q motif"
FT MOTIF 379..382
FT /note="DEAD box"
FT COMPBIAS 81..110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..196
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 685..699
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 706..722
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 775..799
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 266..273
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 808 AA; 89326 MW; 5787CB4324D3D8BB CRC64;
MIIHSSSSNS KKEYTMADDF ITTIDSDDEV SNYGEPSALP KIKDDELDPD FQFDLGGGRS
EGLDLWGGDE VQGVKKGNEP INVDDIIERK RGKPIRAFKD RKRKRDEDAT SEDDLEEDEE
EEGDSNDDSD AAKSGDSEED EMDVDMSEGD GDEEDENEIE SLKREDESDE EEEEEEDDYD
EEGENEVVDS DSESEEETAA EIARKDAFFS SDPTTTDPTL PSSFTAMNLS RPLLRALTSL
QFTAPTPIQA RAIPLALLGR DILGSAVTGS GKTAAFMVPI LERLCYRDRG KGGAACRVLV
LCPTRELAVQ CEAVGKALAE KGGLDVRFAL LVGGLSLNAQ AHTLRTLPDI LIATPGRLID
HLTNTPSFTL SALDVLVIDE ADRMLEAGFT DELEEIIKAC PRSRQTMLFS ATMTDSVDEL
VKLSLDKPIR VFVDPKRNTA RGLTQEFVRI RSDDSRSPSL LALCKRTIRE KCIIFFRSKA
LAHQMRIVFG LFGLKAAELH GNLTQEQRLQ ALNDFKAGTV DYLLATDLAS RGLDIKGVET
VINYDMPGQL AQYTHRVGRT ARAGRKGRSV SLVGEADRKM LKAAIKQAEA DQVRHRIIPS
EAVTAMKEKL EEFKDDIQEI LKEEKEEKLL RQADMEIKKG QNMVEHEAEI FSRPARTWFQ
SGKEKQASKS AGKDAYVGSF PSTGKSAEKE KEKLKRGKYD GLSRRLKRRK MAIEEDAADA
AAARKTEMGI RAAKKNALPK KITEPQPRLE KAGKGKDKKK GKARRVTGGK GSAFDSEGKK
SHEGMRAKPA KVNLEKGKKK GGKGKGRK
