ID   DRS1_PITNO              Reviewed;          20 AA.
AC   P0DTD4;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Dermaseptin-N1 {ECO:0000305};
DE            Short=DRS-N1 {ECO:0000305};
DE   AltName: Full=Dermaseptin-H10 {ECO:0000303|PubMed:23774944};
DE            Short=DRS-H10 {ECO:0000303|PubMed:23774944};
OS   Pithecopus nordestinus (Northeastern Brazilian leaf frog) (Phyllomedusa
OS   nordestina).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Pithecopus.
OX   NCBI_TaxID=2034992;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AMIDATION AT LEU-20, AND
RP   SYNTHESIS.
RC   TISSUE=Skin secretion;
RX   PubMed=23774944; DOI=10.3390/molecules18067058;
RA   Brand G.D., Santos R.C., Arake L.M., Silva V.G., Veras L.M., Costa V.,
RA   Costa C.H., Kuckelhaus S.S., Alexandre J.G., Feio M.J., Leite J.R.;
RT   "The skin secretion of the amphibian Phyllomedusa nordestina: a source of
RT   antimicrobial and antiprotozoal peptides.";
RL   Molecules 18:7058-7070(2013).
CC   -!- FUNCTION: Antimicrobial peptide with moderate activity against both
CC       Gram-positive and Gram-negative bacteria, and important activity
CC       against Leishmania species (L.amazonensis and L.infantum)
CC       (PubMed:23774944). Acts on both Leishmania promastigote and amastigote
CC       forms (PubMed:23774944). Shows activity against E.coli (MIC=17.8 uM),
CC       S.aureus (MIC=32.3 uM) and the phytopathogenic bacterium Xanthomonas
CC       axonopodis (MIC=2 uM) (PubMed:23774944). Shows low cytotoxicity against
CC       mammalian cells in models of peritoneal macrophages (PubMed:23774944).
CC       {ECO:0000269|PubMed:23774944}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23774944}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:23774944}.
CC   -!- MASS SPECTROMETRY: Mass=1982.26; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:23774944};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Dermaseptin subfamily. {ECO:0000305}.
CC   -!- CAUTION: Sequence shown in this entry in copied from Fig.2, and not
CC       from Table 1, which differ. {ECO:0000305|PubMed:23774944}.
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DR   AlphaFoldDB; P0DTD4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR022731; Dermaseptin.
DR   Pfam; PF12121; DD_K; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..20
FT                   /note="Dermaseptin-N1"
FT                   /evidence="ECO:0000269|PubMed:23774944"
FT                   /id="PRO_0000449580"
FT   MOD_RES         20
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:23774944"
SQ   SEQUENCE   20 AA;  1983 MW;  ADDEE153932DC510 CRC64;
     GLWSTIKNVA AAAGKAAIKL