DRS3_PITHY
ID DRS3_PITHY Reviewed; 70 AA.
AC P84596;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Dermaseptin-H3 {ECO:0000303|PubMed:18644413};
DE Short=DRS-H3 {ECO:0000303|PubMed:18644413};
DE AltName: Full=DShypo 01 {ECO:0000303|PubMed:16844081};
DE AltName: Full=Dermaseptin DPh-1 {ECO:0000303|PubMed:16963159};
DE Flags: Precursor; Fragment;
OS Pithecopus hypochondrialis (Orange-legged leaf frog) (Phyllomedusa
OS hypochondrialis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC Pithecopus.
OX NCBI_TaxID=317381;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 46-70, FUNCTION,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION
RP AT LEU-70.
RC TISSUE=Skin, and Skin secretion;
RX PubMed=16844081; DOI=10.1016/j.bbrc.2006.06.168;
RA Brand G.D., Leite J.R.S.A., de Sa Mandel S.M., Mesquita D.A., Silva L.P.,
RA Prates M.V., Barbosa E.A., Vinecky F., Martins G.R., Galasso J.H.,
RA Kuckelhaus S.A.S., Sampaio R.N.R., Furtado J.R. Jr., Andrade A.C.,
RA Bloch C. Jr.;
RT "Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia).";
RL Biochem. Biophys. Res. Commun. 347:739-746(2006).
RN [2]
RP PROTEIN SEQUENCE OF 46-70, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION AT LEU-70.
RC TISSUE=Skin secretion;
RX PubMed=16963159; DOI=10.1016/j.peptides.2006.08.005;
RA Conceicao K., Konno K., Richardson M., Antoniazzi M.M., Jared C.,
RA Daffre S., de Camargo A.C.M., Pimenta D.C.;
RT "Isolation and biochemical characterization of peptides presenting
RT antimicrobial activity from the skin of Phyllomedusa hypochondrialis.";
RL Peptides 27:3092-3099(2006).
RN [3]
RP NOMENCLATURE.
RX PubMed=18644413; DOI=10.1016/j.peptides.2008.06.017;
RA Amiche M., Ladram A., Nicolas P.;
RT "A consistent nomenclature of antimicrobial peptides isolated from frogs of
RT the subfamily Phyllomedusinae.";
RL Peptides 29:2074-2082(2008).
CC -!- FUNCTION: Has antibacterial activity against the Gram-negative bacteria
CC E.coli and P.aeruginosa, and the Gram-positive bacteria S.aureus and
CC M.luteus. Has antiprotozoal activity against L.amazonensis. No
CC hemolytic activity. {ECO:0000269|PubMed:16844081,
CC ECO:0000269|PubMed:16963159}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16844081,
CC ECO:0000269|PubMed:16963159}.
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:16844081, ECO:0000269|PubMed:16963159}.
CC -!- MASS SPECTROMETRY: Mass=2409.41; Mass_error=0.1; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:16844081};
CC -!- MASS SPECTROMETRY: Mass=2408.4; Mass_error=0.5; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:16963159};
CC -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC Dermaseptin subfamily. {ECO:0000255}.
CC -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC URL="https://wangapd3.com/database/query_output.php?ID=0763";
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DR AlphaFoldDB; P84596; -.
DR TCDB; 1.C.52.1.20; the dermaseptin (dermaseptin) family.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR InterPro; IPR022731; Dermaseptin.
DR InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR InterPro; IPR016322; FSAP.
DR Pfam; PF12121; DD_K; 1.
DR Pfam; PF03032; FSAP_sig_propep; 1.
DR PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE 1: Evidence at protein level;
KW Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW Cleavage on pair of basic residues; Direct protein sequencing; Secreted;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..43
FT /evidence="ECO:0000269|PubMed:16844081"
FT /id="PRO_0000248492"
FT PEPTIDE 46..70
FT /note="Dermaseptin-H3"
FT /evidence="ECO:0000269|PubMed:16844081,
FT ECO:0000269|PubMed:16963159"
FT /id="PRO_0000248493"
FT REGION 25..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 70
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:16844081,
FT ECO:0000269|PubMed:16963159"
FT NON_TER 70
FT /evidence="ECO:0000305|PubMed:16844081"
SQ SEQUENCE 70 AA; 7761 MW; 78EEA0D62C4C863D CRC64;
MAFLKKSLFL VLFLGMVSLS ICEEEKRENE DEELQEDDEQ SEMKRGLWST IKNVGKEAAI
AAGKAALGAL
