ID   DRS4_AGASP              Reviewed;          75 AA.
AC   A0A5P9K6A8;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2020, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Dermaseptin-SP4 {ECO:0000303|PubMed:31671555};
DE            Short=DRS-SP4 {ECO:0000303|PubMed:31671555};
DE   Flags: Precursor;
OS   Agalychnis spurrelli (Gliding leaf frog) (Agalychnis litodryas).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Agalychnis.
OX   NCBI_TaxID=317303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   SYNTHESIS OF 46-72, SUBCELLULAR LOCATION, AND AMIDATION AT PRO-72.
RC   TISSUE=Skin secretion;
RX   PubMed=31671555; DOI=10.3390/biom9110667;
RA   Proano-Bolanos C., Blasco-Zuniga A., Almeida J.R., Wang L.,
RA   Llumiquinga M.A., Rivera M., Zhou M., Chen T., Shaw C.;
RT   "Unravelling the skin secretion peptides of the gliding leaf frog,
RT   Agalychnis spurrelli (Hylidae).";
RL   Biomolecules 9:1-20(2019).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SYNTHESIS OF 46-73, IDENTIFICATION BY
RP   MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND 3D-STRUCTURE MODELING.
RC   TISSUE=Skin secretion;
RX   PubMed=31422479; DOI=10.1007/s00894-019-4141-1;
RA   Cuesta S., Gallegos F., Arias J., Pilaquinga F., Blasco-Zuniga A.,
RA   Proano-Bolanos C., Rivera M., Meneses L.;
RT   "Molecular modeling of four dermaseptin-related peptides of the gliding
RT   tree frog Agalychnis spurrelli.";
RL   J. Mol. Model. 25:260-260(2019).
CC   -!- FUNCTION: Antimicrobial peptide with activity against Gram-positive and
CC       Gram-negative bacteria and fungi (PubMed:31671555, PubMed:31422479).
CC       Has been tested against E.coli (MIC=47.25-128 uM), S.aureus (MIC=189-
CC       512 uM), K.pneumoniae (MIC=189 uM) and C.albicans (MIC>189 uM)
CC       (PubMed:31671555, PubMed:31422479). Probably acts by disturbing
CC       membrane functions with its alpha-helical amphipathic structure
CC       (Probable). May penetrate bacterial membranes, but stay at the
CC       mammalian membrane surface (Probable). Shows a weak hemolytic activity
CC       (PubMed:31671555). {ECO:0000269|PubMed:31422479,
CC       ECO:0000269|PubMed:31671555, ECO:0000305|PubMed:31422479}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31422479,
CC       ECO:0000269|PubMed:31671555}. Target cell membrane
CC       {ECO:0000250|UniProtKB:P31107}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:31671555}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Dermaseptin subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MK532482; QFU19632.1; -; mRNA.
DR   AlphaFoldDB; A0A5P9K6A8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR022731; Dermaseptin.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   InterPro; IPR016322; FSAP.
DR   Pfam; PF12121; DD_K; 1.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
DR   PIRSF; PIRSF001822; Dermaseptin_precursor; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Cytolysis; Fungicide; Hemolysis;
KW   Immunity; Innate immunity; Membrane; Secreted; Signal;
KW   Target cell membrane; Target membrane.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..45
FT                   /evidence="ECO:0000305|PubMed:31671555"
FT                   /id="PRO_0000449989"
FT   PEPTIDE         46..72
FT                   /note="Dermaseptin-SP4"
FT                   /evidence="ECO:0000305|PubMed:31671555"
FT                   /id="PRO_0000449990"
FT   PROPEP          74..75
FT                   /evidence="ECO:0000305|PubMed:31671555"
FT                   /id="PRO_0000449991"
FT   MOD_RES         72
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000305|PubMed:31671555"
SQ   SEQUENCE   75 AA;  8386 MW;  E2D21635B946536C CRC64;
     MAFLKKSLFL VLFLGLVSLS MCEEEKRENE VEEEQEDDEQ SELRRSLWSS IKDMAAAAGR
     AALNAVNGIL NPGEQ